Phytochemical Analysis and Antioxidant Activity Assessment of Croton linearifolius Stem Extracts

Cleia Teixeira Santos de Franca; Marcel Mark da Silva  Passos; Matheus Andrade Rocha Costa; Esaul Lucas Oliveira; Simone Andrade Gualberto

doi:10.5433/1679-0375.2025.v46.50875

Phytochemical Analysis and Antioxidant Activity Assessment of Croton linearifolius Stem Extracts

Authors

Cleia Teixeira Santos de Franca Southwest Bahia State University https://orcid.org/0000-0002-5566-1469
Marcel Mark da Silva Passos Universidade Federal de Catalão https://orcid.org/0000-0002-2832-9882
Matheus Andrade Rocha Costa Universidade Federal de Pernambuco https://orcid.org/0000-0003-0194-3310
Esaul Lucas Oliveira Southwest Bahia State University https://orcid.org/0009-0008-8594-7637
Simone Andrade Gualberto Southwest Bahia State University https://orcid.org/0000-0002-1753-002X

DOI:

https://doi.org/10.5433/1679-0375.2025.v46.50875

Keywords:

Croton linearifolius, antioxidant activity, phenolic compounds, flavonoids

Abstract

Croton linearifolius is a species with promising medicinal potential. This study investigated the antioxidant activity, total phenolic and flavonoid contents, and conducted chemical prospecting of its ethanolic stem extract and fractions. The extract was obtained by exhaustive percolation with 70% hydroethanolic solution, followed by liquid-liquid partitioning using hexane, dichloromethane, and ethyl acetate. In vitro tests were used for chemical analysis, and antioxidant activity was evaluated by DPPH and FRAP methods. The ethyl acetate fraction showed the highest antioxidant activity (80.62% in DPPH), with elevated phenolic and flavonoid levels, indicating potential for herbal medicine development.

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Author Biographies

Cleia Teixeira Santos de Franca, Southwest Bahia State University

Master in Chemistry from the Postgraduate Program in Chemistry, UESB, Itapetinga, BA, Brazil.

Marcel Mark da Silva Passos, Universidade Federal de Catalão

Master in Environmental Sciences, Prof. UFCAT, Catalão, Goiás, Brazil

Matheus Andrade Rocha Costa, Universidade Federal de Pernambuco

PhD candidate in the Energy and Nuclear Technologies program at the Federal University of Pernambuco (UFPE), Recife, PE, Brazil.

Esaul Lucas Oliveira, Southwest Bahia State University

PhD candidate in Food Engineering and Science, UESB, Itapetinga, BA, Brazil.

Simone Andrade Gualberto, Southwest Bahia State University

Prof. Dr. State University of Southwest Bahia, Itapetinga, BA, Brazil

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