Optimization of ethanol production by Zymomonas mobilis in sugar cane molasses fermentation

Optimization of ethanol production by Zymomonas mobilis in sugar cane molasses fermentation

Authors

  • Doumit Camilios Neto Universidade Estadual de Londrina
  • João Batista Buzato Universidade Estadual de Londrina
  • Maria Antonia Pedrine Colabone Celligoi Universidade Estadual de Londrina
  • Marcos Roberto Oliveira Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/1679-0375.2005v26n1p17

Keywords:

Ethanol, Zymomonas mobilis, Molasses, Factorial Design

Abstract

The present study aimed at the optimization of the ethanol production by Zymomonas mobilis CP4,

during the fermentation of sugar cane molasses. As for the optimization process, the response surface

methodology was applied, using a 33 incomplete factorial design, being the independent variables: total

reducing sugar (TRS) concentration in the molasses from 10, 55 and 100 g/L (x1); yeast extract concentration from 2, 11 and 20 g/L (x2), and fermentation time from 6, 15 and 24 hours (x3). The dependant variables or answers were the production and productivity of ethanol. By the analysis of the results, a good adjustment of the model to the experimental data was obtained. In the levels studied, the best condition for the production of ethanol was with 100 g/L TRS in the syrup, 2.0 g/L of yeast extract and the fermentation time between 20 and 24 hours, producing 30 g/L of ethanol.

 

 

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Author Biographies

Doumit Camilios Neto, Universidade Estadual de Londrina

Aluno do Mestrado em Biotecnologia.

João Batista Buzato, Universidade Estadual de Londrina

Docentes do Departamento de Bioquímica e Biotecnologia – CCE – Universidade Estadual de Londrina. Caixa Postal:6001-CEP:86051-990, Londrina/Pr. Brasil.

Maria Antonia Pedrine Colabone Celligoi, Universidade Estadual de Londrina

Docentes do Departamento de Bioquímica e Biotecnologia – CCE – Universidade Estadual de Londrina. Caixa Postal:6001-CEP:86051-990, Londrina/Pr. Brasil.

Marcos Roberto Oliveira, Universidade Estadual de Londrina

Alunos do Mestrado em Biotecnologia.

Published

2005-07-15

How to Cite

Camilios Neto, D., Buzato, J. B., Celligoi, M. A. P. C., & Oliveira, M. R. (2005). Optimization of ethanol production by Zymomonas mobilis in sugar cane molasses fermentation. Semina: Ciências Exatas E Tecnológicas, 26(1), 17–22. https://doi.org/10.5433/1679-0375.2005v26n1p17

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Original Article

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