Application of loofa sponge (Luffa cylindrica) as carrier for invertase immobilization for invert sugar syrup production

Application of loofa sponge (Luffa cylindrica) as carrier for invertase immobilization for invert sugar syrup production

Authors

  • Evandro Cesar Poças Universidade Estadual de Londrina
  • João Batista Buzato Universidade Estadual de Londrina
  • Maria Antonia Pedrine Colabone Celligoi Universidade Estadual de Londrina
  • Doumit Camilios Neto Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/1679-0375.2004v25n1p53

Keywords:

Immobilization, Invertase, Luffa Cylindrica.

Abstract

Invertase immobilization in loofa sponge (Luffa cylindrica) for invert sugar syrup production has been carried out. Loofa sponge was treated with NaIO4 followed by the use of diamine compound, glutaraldehyde and enzyme immobilization. The best results obtained were: invertase immobilized activity: 4.510 (mmols/g matrix x min x mL); enzyme coupling efficiency: 0.428%, recovered activity: 1.5% and the extent of hydrolysis was of 2.6x10-3%.h-1.

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Author Biographies

Evandro Cesar Poças, Universidade Estadual de Londrina

Graduando em química - Universidade Estadual de Londrina.

João Batista Buzato, Universidade Estadual de Londrina

Docente do Dep. Bioquímica e Biotecnologia - Universidade Estadual de Londrina.

Maria Antonia Pedrine Colabone Celligoi, Universidade Estadual de Londrina

Docente do Dep. Bioquímica e Biotecnologia - Universidade Estadual de Londrina.

Doumit Camilios Neto, Universidade Estadual de Londrina

Mestrando em Biotecnologia - Universidade Estadual de Londrina.

Published

2004-07-15

How to Cite

Poças, E. C., Buzato, J. B., Celligoi, M. A. P. C., & Camilios Neto, D. (2004). Application of loofa sponge (Luffa cylindrica) as carrier for invertase immobilization for invert sugar syrup production. Semina: Ciências Exatas E Tecnológicas, 25(1), 53–58. https://doi.org/10.5433/1679-0375.2004v25n1p53

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Original Article

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