An alternative to evaluate the efficiency of in vitro culture medium using a logistic regression model

An alternative to evaluate the efficiency of in vitro culture medium using a logistic regression model

Authors

  • Marcelo Angelo Cirillo Universidade Federal de Lavras
  • Daniel Furtado Ferreira Universidade Federal de Lavras
  • Patrícia Schober Gonçalves Lima Universidade Federal de Lavras

DOI:

https://doi.org/10.5433/1679-0375.2003v24n1p101

Keywords:

In vitro, Logistic model, Probabilities.

Abstract

The evaluation of a culture medium for the in vitro culture of a species is performed using its physical and/or chemical properties. However, the analysis of the experimental results makes it possible to evaluate its quality. In this sense, this work presents an alternative using a logistic model to evaluate the culture medium to be used in vitro. The probabilities provided by this model will be used as a medium evaluator index. The importance of this index is based on the formalization of a statistical criterion for the selection of the adequate culture medium to be used on in vitro culture without excluding its physical and/or chemical properties. To demonstrate this procedure, an experiment determining the ideal medium for the in vitro culture of primary explants of Ipeca [Psychotria ipecacuanha (Brot.) Stokes] was evaluated. The differentiation of the culture medium was based on the presence and absence of the growth regulator BAP (6-benzilaminopurine). A logistic model was adjusted as a function of the weight of fresh and dry matter. Minimum, medium and maximum probabilities obtained with this model showed that the culture medium containing BAP was the most adequate for the explant growth. Due to the high discriminative power of these mediums, detected by the model, their use is recommended as an alternative to select culture medium for similar experiments.

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Author Biographies

Marcelo Angelo Cirillo, Universidade Federal de Lavras

Graduação em Bacharelado em Estatística pela UNESP - Universidade Estadual Paulista Júlio de Mesquita Filho, mestrado em Estatística e Experimentação Agropecuária pela Universidade Federal de Lavras e doutorado em Estatística e Experimentação Agropecuária pela Universidade Federal de Lavras. Professor da Universidade Federal de Lavras.

 

Daniel Furtado Ferreira, Universidade Federal de Lavras

Prof. Adjunto do Departamento de Ciências Exatas – UFLA, Bolsista: CNPq na modalidade de produtividade em pesquisa.

Patrícia Schober Gonçalves Lima, Universidade Federal de Lavras

Mestre em Genética/Melhoramento Vegetal – Departamento de Biologia - UFLA

Published

2004-07-15

How to Cite

Cirillo, M. A., Ferreira, D. F., & Lima, P. S. G. (2004). An alternative to evaluate the efficiency of in vitro culture medium using a logistic regression model. Semina: Ciências Exatas E Tecnológicas, 24(1), 101–110. https://doi.org/10.5433/1679-0375.2003v24n1p101

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Original Article

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