Concentração inibitória mínima de óleos essenciais contra Staphylococcus aureus isolados de cães com otite externa

Autores

DOI:

https://doi.org/10.5433/1679-0359.2021v42n6SUPL2p3837

Palavras-chave:

Fitoterapia, Otopatias, Antibacterianos, Antimicrobianos naturais, CIM.

Resumo

A otite canina externa é uma doença que afeta o meato acústico externo de até 20% dos cães em algum período de suas vidas, sem predileção por raça, idade ou sexo. Trata-se de uma doença multifatorial que tem Staphylococcus aureus como um dos principais agentes etiológicos. Antimicrobianos constituem a base para o tratamento desta enfermidade, entretanto, devido ao incremento da resistência antimicrobiana, os fármacos convencionais tem se tornado pouco eficazes, o que requer a busca por terapias alternativas. Neste contexto, os óleos essenciais (OE) apresentam grande potencial terapêutico devido a sua ampla ação antimicrobiana. Este trabalho visou detectar a concentração inibitória mínima (CIM), a partir da qual foram obtidos os índices de MIC50 e MIC90 para a gentamicina e para os OE de Eugenia caryophyllata, Thymus vulgaris, Cymbopogon winterianus, Cymbopogon citratus e Cinnamomum cassia contra 62 Staphylococcus aureus isolados do meato acústico externo de cães otopatas. Todos os OE mostraram ação antibacteriana contra os microrganismos estudados e foram detectadas as seguintes CIM50 e CIM90: Eugenia caryophyllata, 2,42 mg mL-1e 7,45 mg mL-1; Thymus vulgaris, 9,51 mg mL-1 e 22,94 mg mL-1; Cymbopogon winterianus, 26,78 mg mL-1 e 157,79 mg mL-1; Cinnamomum cassia e Cymbopogon citratus CM menores que 16,48 e 27,81 mg mL-1, respectivamente já que estes apresentaram CIM única para todos os isolados testados. Para a gentamicina, foram obtidas CIM50 e CIM90 1ug mL-1 e 8 ug/m, respectivamente. Ademais, a faixa de CIM encontrada para o antibiótico variou de 0,5 a 128 ug mL-1, e os isolados foram classificados em susceptíveis [48 isolados (77,41%) - CIM na faixa de 0,5-4 ug mL-1], intermediários [8 isolados (12,90%) - (CIM = 8ug mL-1] e resistentes [6 isolados (9,68%) - CIM maior, menor 16]. Os resultados encontrados mostraram que os isolados de micro-organismos testados tem potencial para desenvolver resistência antimicrobiana a terapêutica tradicional e que os OE, de acordo com os resultados in vitro, apresentam potencial de uso terapêutico, entretanto futuros estudos devem ser realizados para avaliar a eficácia dos OE in vivo.

Métricas

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Biografia do Autor

Geraldo Márcio Costa, Universidade Federal de Lavras

Prof., Faculdade de Zootecnia e Medicina Veterinária, Universidade Federal de Lavras, DCV, UFLA, Lavras, MG, Brasil.

Ingrid Alexandre Prado, Universidade Federal de Lavras

Discente de Pós-Graduação, DCV, UFLA, Lavras, MG, Brasil.

Glei dos Anjos de Carvalho-Castro, Universidade Federal de Lavras

Prof., Faculdade de Zootecnia e Medicina Veterinária, Universidade Federal de Lavras, DCV, UFLA, Lavras, MG, Brasil.

Glaúcia Frasnelli Mian, Universidade Federal de Lavras

Profa, Faculdade de Zootecnia e Medicina Veterinária, Universidade Federal de Lavras, DCV, UFLA, Lavras, MG, Brasil.

Carlos Artur Lopes Leite, Universidade Federal de Lavras

Prof., Faculdade de Zootecnia e Medicina Veterinária, Universidade Federal de Lavras, DCV, UFLA, Lavras, MG, Brasil.

Dircéia Aparecida Costa Custódio, Universidade Federal de Lavras

Pesquisador, DCV, UFLA, MG, Brasil.

Roberta Hilsdorf Piccoli, Universidade Federal de Lavras

Profa, Departamento de Ciência dos Alimentos, DCA, UFLA, MG, Brazil.

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2021-10-08

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Costa, G. M., Prado, I. A., Carvalho-Castro, G. dos A. de, Mian, G. F., Leite, C. A. L., Custódio, D. A. C., & Piccoli, R. H. (2021). Concentração inibitória mínima de óleos essenciais contra Staphylococcus aureus isolados de cães com otite externa. Semina: Ciências Agrárias, 42(6SUPL2), 3837–3854. https://doi.org/10.5433/1679-0359.2021v42n6SUPL2p3837

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