Effect of the addition of sodium caseinate on the viability of cryopreserved buffalo semen
DOI:
https://doi.org/10.5433/1679-0359.2020v41n5supl1p2209Keywords:
BSP, Buffalo, Casein, Cryopreservation, Sperm.Abstract
The use of cooled semen in artificial insemination operations results in higher pregnancy rates than the use of frozen semen. This result seems to be related to the more severe damage triggered by the freezing process than that observed during refrigeration. Due to its ability to bind to sperm-binding proteins and calcium ions, sodium caseinate has been studied as a substance capable of preventing early sperm capacitation, a significant cause of the decreased pregnancy rate resulting from the use of frozen semen. The first objective of this study was to evaluate whether a commercial egg yolk diluent developed for frozen bovine semen could be used for buffalo semen cryopreservation; the second objective was to investigate the effect of this diluent in combination with sodium caseinate during the procedures of buffalo sperm cryopreservation using flow cytometry and computer-assisted sperm analysis. In the first part of the study, comparing the results of spermatic kinetics and plasma and acrosomal membrane integrity, it was observed that the freezing process resulted in more cell damage than the cooling process. In the second part of the study, no effects of the addition of sodium caseinate to the egg yolk diluent were observed. From the results of the present study, it was possible to conclude that the egg yolk-based diluent was suitable for buffalo semen cryopreservation and that the addition of sodium caseinate did not decrease the harmful effects related to seminal cryopreservation.Downloads
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References
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Silva, J. C. B., Silva, M. R., Silva, R. G. da, Nogueira, E., Oliveira, L. O. F. de, Abreu1, U. G. P. de,... Rodrigues, W. B. (2019). Use of bovine chilled semen: breaking paradigms. Brazilian Journal of Animal Reproduction, 43(2), 284-288.
Silva, M. A. C. da, Seidel, G. E., Squires, E. L., Graham, J. K., & Carnevale, E. M. (2012). Effects of components of semen extenders on the binding of stallion spermatozoa to bovine or equine zonae pellucidae. Reproduction, 143(5), 577-585. doi: 10.1530/REP-11-0099
Singh, M., Ghosh, S. K., Prasad, J. K., Kumar, A., Tripathi, R. P., Bhure, S. K., & Srivastava, N. (2014). Seminal PDC-109 protein vis-à-vis cholesterol content and freezability of buffalo Spermatozoa. Animal Reproduction Science, 144(1-2), 22-29. doi: 10.1016/j.anireprosci.2013.10.016
Souza, J. D. S., & Ferreira, W. M. (2007). The role of vitamin E in animal nutrition and reproduction - means of defense against free radicals. Nutritime Electronic Journal, 4(3), 456-461.
Tarragó, O. F. B. (2017). Sêmen refrigerado bovino reduz os danos espermáticos e aumenta a taxa de prenhez na IATF?. Tese de doutorado, Universidade de São Paulo, São Paulo, SP, Brasil.
Ugur, M. R., Abdelrahman, A. S., Evans, H. C., Gilmore, A. A., Hitit, M., Arifiantini, R. I., Purwantara, B., Kaya, A., & Memili, E. (2019). Advances in cryopreservation of bull sperm. Frontiers in Veterinary Science, 6, 268. doi: 10.3389/fvets.2019.00268
Watson, P. F. (1995). Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reproduction, Fertility and Development, 7(4), 871-891. doi: 10.1071/RD9950871
Watson, P. F. (2000). The causes of reduced fertility with cryopreserved semen. Animal Reproduction Science, 60-61, 481-492. doi: 10.1016/S0378-4320(00)00099-3
Zorzetto, M. F. (2013). Avaliação do sêmen de búfalos em três meios de criopreservação. Dissertação de mestrado, Faculdade de Medicina Veterinária e Zootecnia, Botucatu, SP, Brasil.
Bailey, J. L., Blodeau, J. F., & Cormier, N. (2000). Semen cryopreservation in domestic animals: A damaging and capacitating phenomenon minireview. Journal of Andrology, 21(1), 1-7. doi: 10.1002/j. 1939-4640.2000.tb03268.x
Batellier, F., Magistrini, M., Fauquant, J., & Palmer, E. (1997). Effect of milk fractions on survival of equine spermatozoa. Theriogenology, 48(3), 391-410. doi: 10.1016/s0093-691x(97)00250-1
Battelier, F., Duchamp, G., Vidament, M., Arnaud, G., Palmer, E., & Magistrini, M. (1998). Delayed insemination is successful with a new extender for storing fresh semen at 15 °C under aerobic conditions. Theriogenology, 50(2), 229-236. doi: 10.1016/s0093-691x(98)00130-7
Battelier, F., Vidament, M., Fauquant, J., Duchamp, G., Arnaud, G., Yvon, J. M., & Magistrini, M. (2001). Advances in cooled semen technology. Animal Reproduction Science, 68(3-4), 181-190. doi: 10.1016/ S0378-4320(01)00155-5
Bergeron, A., Brindle, Y., Blondin, P., & Manjunath, P. (2007). Milk caseins decrease the binding of the major bovine seminal plasma proteins to sperm and prevent lipid loss from the sperm membrane during sperm storage. Biology of Reproduction, 77(1), 120-126. doi: 10.1095/biolreprod.106.058248
Borges-Silva, J. C., Silva, M. R., Marinho, D. B., Nogueira, E., Sampaio, D. C., Oliveira, L. O. F.,... Sartori, R. (2016). Cooled semen for fixed-time artificial insemination in beef cattle. Reproduction, Fertility and Development, 28(7), 1004-1008. doi: 10.1071/RD14185
Colégio Brasileiro de Reprodução Animal. (2013). Manual para exame andrológico e avaliação de semen animal (3a ed.). Belo Horizonte: CBRA.
Diniz, J. V. A. (2017). Utilização do caseinato de sódio na congelação de sêmen bovino, Tese de doutorado, Faculdade de Medicina Veterinária e Zootecnia, Botucatu, SP, Brasil.
Emamverdi, M., Zhandi, M., Shahneh, A. Z., Sharafi, M., Akhlaghi, A., Motlagh, M. K.,… Davachi, N. D. (2015). Flow cytometric and microscopic evaluation of post-thawed ram semen cryopreserved in chemically defined home-made or commercial extenders. Animal Production Science, 55(4), 551-558. doi: 10.1071/AN13215
Fox, P. F., & McSweeney, P. L. H. (1998). Milk proteins. In Dairy Chemistry and Biochemistry, (pp. 230-239). New York, NY: Kluwer Academic/Plenum Publishers.
Freitas-Dell'Aqua, C. P., Guasti, P. N., Monteiro, G. A., Maziero, R. R. D., Dell'Aqua, J. A., & Papa, F. O. (2012). Flow cytometric analysis of fertile and subfertile frozen stallion spermatozoa. Animal Reproduction, 9(4), 941.
Guasti, P. N., Freitas-Dell'Aqua, C. P., Maziero, R. R. D., Hartwig, F. P., Monteiro, G. A., Lisboa, F. P., & Papa, F. O. (2012). Validation of flow cytometry for assessment of membrane lipid peroxidation of equine spermatozoa. Animal Reproduction, 9(4), 929.
Lusignan, M. F., Bergeron, A., Lafleur, M., & Manjunath, P. (2011). The major proteins of bovine seminal plasma interact with caseins and whey proteins of milk extender. Biology of Reproduction, 85(3), 457-464. doi: 10.1095/biolreprod.110.089961
Manjunath, P. (2012). New insights into the understanding of the mechanism of sperm protection by extender components. Animal Reproduction, 9(4), 809-815.
Olson, S. E., & Seidel, G. E., Jr. (2000). Reduced oxygen tension and EDTA improve bovine zygote development in a chemically defined medium. Journal of Animal Science, 78(1), 152-157. doi: 10.2527/ 2000.781152x
Pagl, R., Aurich, J. E., Müller-Schlösser, F., Kankkofer, M., & Aurich, C. (2006). Comparison of an extender containing defined milk protein fractions with a skim milk-based extender for storage of equine semen at 5 °C. Theriogenology, 66(5), 1115-1122. doi: 10.1016/j.theriogenology.2006.03.006
Papa, P. M., Maziero, R. D., Guasti, P. N., Junqueira, C. R., Freitas-Dell'Aqua, C. P., Papa, F. O.,… Dell'Aqua Jr, J. A. (2015). Effect of glycerol on the viability and fertility of cooled bovine semen. Theriogenology, 83(1), 107-113. doi: 10.1016/j.theriogenology.2014.08.009
Parrish, J. J., Susko-Parrish, J., Winer, M. A., & First, N. L. (1988). Capacitation of bovine sperm by heparin. Biology of Reproduction, 38(5), 1171-1180. doi: 10.1095/biolreprod38.5.1171
Silva, J. C. B., Silva, M. R., Silva, R. G. da, Nogueira, E., Oliveira, L. O. F. de, Abreu1, U. G. P. de,... Rodrigues, W. B. (2019). Use of bovine chilled semen: breaking paradigms. Brazilian Journal of Animal Reproduction, 43(2), 284-288.
Silva, M. A. C. da, Seidel, G. E., Squires, E. L., Graham, J. K., & Carnevale, E. M. (2012). Effects of components of semen extenders on the binding of stallion spermatozoa to bovine or equine zonae pellucidae. Reproduction, 143(5), 577-585. doi: 10.1530/REP-11-0099
Singh, M., Ghosh, S. K., Prasad, J. K., Kumar, A., Tripathi, R. P., Bhure, S. K., & Srivastava, N. (2014). Seminal PDC-109 protein vis-à-vis cholesterol content and freezability of buffalo Spermatozoa. Animal Reproduction Science, 144(1-2), 22-29. doi: 10.1016/j.anireprosci.2013.10.016
Souza, J. D. S., & Ferreira, W. M. (2007). The role of vitamin E in animal nutrition and reproduction - means of defense against free radicals. Nutritime Electronic Journal, 4(3), 456-461.
Tarragó, O. F. B. (2017). Sêmen refrigerado bovino reduz os danos espermáticos e aumenta a taxa de prenhez na IATF?. Tese de doutorado, Universidade de São Paulo, São Paulo, SP, Brasil.
Ugur, M. R., Abdelrahman, A. S., Evans, H. C., Gilmore, A. A., Hitit, M., Arifiantini, R. I., Purwantara, B., Kaya, A., & Memili, E. (2019). Advances in cryopreservation of bull sperm. Frontiers in Veterinary Science, 6, 268. doi: 10.3389/fvets.2019.00268
Watson, P. F. (1995). Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reproduction, Fertility and Development, 7(4), 871-891. doi: 10.1071/RD9950871
Watson, P. F. (2000). The causes of reduced fertility with cryopreserved semen. Animal Reproduction Science, 60-61, 481-492. doi: 10.1016/S0378-4320(00)00099-3
Zorzetto, M. F. (2013). Avaliação do sêmen de búfalos em três meios de criopreservação. Dissertação de mestrado, Faculdade de Medicina Veterinária e Zootecnia, Botucatu, SP, Brasil.
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2020-08-07
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Silva, F. E. da, Carvalho, J. C., Dell’Aqua, C. de P. F., Papa, F. O., Henry, M. R. J. M., Oba, E., & Ferreira, J. C. P. (2020). Effect of the addition of sodium caseinate on the viability of cryopreserved buffalo semen. Semina: Ciências Agrárias, 41(5supl1), 2209–2218. https://doi.org/10.5433/1679-0359.2020v41n5supl1p2209
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