Efeito da inclusão de quercetina ou butil-hidroxitolueno na qualidade do sêmen de carneiro resfriado ou congelado
DOI:
https://doi.org/10.5433/1679-0359.2022v43n2p841Palavras-chave:
Antioxidante, Criopreservação dano oxidativo, Peroxidação lipídica, Pequenos ruminantes, Ovinos.Resumo
O resfriamento e o congelamento causam danos físicos e químicos aos espermatozoides por choque térmico e estresse oxidativo. Portanto, este estudo teve como objetivo avaliar o efeito da inclusão de dois antioxidantes em um diluente à base de gema de ovo sobre os parâmetros espermáticos do sêmen ovino resfriado e congelado. Trinta carneiros tiveram o sêmen coletado e processado em dois experimentos consecutivos para testar a inclusão de diferentes concentrações de quercetina e hidroxitolueno butilado (BHT) em diluente de sêmen à base de gema de ovo. O DMSO foi adicionado como solvente ao diluente de sêmen em uma proporção de 1 mL de DMSO parra 90 mg de quercetina e 1 Ml de DMSO para 880 mg de BHT. Após a coleta, o sêmen foi diluído a 200 × 106 espermatozoides móveis/mL (Controle) e dividido em diferentes grupos em cada experimento. Experimento 1, Quercetina (Q5, 5 ug / mL; Q10, 10 µg / mL; Q15, 15 ug / mL) ou DMSO (DMSO1, 0,055 uL de DMSO por ml; DMSO2, 0,165 uL de DMSO / mL) foram adicionados ao extensor. Experimento 2, BHT (BHT1, 0,5 ug / mL; BHT2, 1 ug / mL; BHT3, 1,5 ug / mL) ou DMSO (DMSO3, 0,375 uL de DMSO por ml; DMSO4, 1,125 uL de DMSO / mL) foram adicionados à o extensor. Após a diluição, o sêmen foi dividido em duas alíquotas. O primeiro foi resfriado a 5 ° C por 24h, enquanto o segundo foi congelado. Os parâmetros de motilidade espermática e integridade da membrana plasmática (PMI) foram avaliados, imediatamente após a diluição (0h) e 24h após o resfriamento e nas amostras congeladas, pelo CASA e microscopia de epifluorescência, respectivamente. A inclusão de quercetina ou BHT não afetou os parâmetros de motilidade espermática e PMI de espermatozoides frescos, resfriados ou congelados (P < 0,05). Portanto, a inclusão de quercetina e BHT não beneficiou os parâmetros espermáticos do sêmen ovino submetido a armazenamento líquido a 5 ° C por 24h ou protocolo de congelamento no presente estudo. No entanto, mais estudos são necessários para testar o efeito desses antioxidantes na fertilidade do sêmen ovino criopreservado.Downloads
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Referências
Allai, L., Druart, X., Öztürk, M., BenMoula, A., Nasser, B., & Amiri, B. (2016). Protective effects of Opuntia ficus-indica extract on ram sperm quality, lipid peroxidation and DNA fragmentation during liquid storage. Animal Reproduction Science, 175, 1-9. doi: 10.1016/j.anireprosci.2016.09.013
Amidi, F., Pazhohan, A., Shabani, N. M., Khodarahmian, M., & Nekoonam, S. (2016). The role of antioxidants in sperm freezing: a review. Cell and Tissue Banking, 17(4), 745-756. doi: 10.1007/s10561 -016-9566-5
Appiah, M. O., Li, W., Zhao, J., Liu, H., Dong, Y., Xiang, J.,… Lu, W. (2020). Quercetin supplemented casein-based extender improves the post-thaw quality of rooster semen. Cryobiology, 94, 57-65. doi: 10. 1016/j.cryobiol.2020.04.010
Avdatek, F., Yeni, D., Inanc, M. E., Çil, B., Tuncer, B. P., Turkmen, R., & Tasdemir, U. (2018). Supplementation of quercetin for advanced DNA integrity in bull semen cryopreservation. Andrologia, 50(4), 1-7. doi: 10.1111/and.12975
Banday, M. N., Lone, F. A., Rasool, F., Rashid, M., & Shikari, A. (2017). Use of antioxidants reduce lipid peroxidation and improve quality of crossbred ram sperm during its cryopreservation. Cryobiology, 74, 25-30. doi: 10.1016/j.cryobiol.2016.12.008
Ben, A. F., Zribi, N., & Ammar-Keskes, L. (2011). Antioxidative potential of Quercetin against hydrogen peroxide induced oxidative stress in spermatozoa in vitro. Andrologia, 43(4), 261-265. doi: 10.1111/j. 1439-0272.2010.01063.x
Boots, A. W., Haenen, G. R. M. M., & Bast, A. (2008). Health effects of quercetin: from antioxidant to nutraceutical. European Journal of Pharmacology, 585(2-3), 325-337. doi: 10.1016/j.ejphar.2008.03.0 08
Brito, L. F. C. (2007). Evaluation of stallion sperm morphology. Clinical Techiniques in Equine Practice, 6(4), 249-264. doi: 10.1053/j.ctep.2007.09.004
Bucak, M. N., Sariozkan, S., Tuncer, P. B., Sakin, F., Atessahin, A., Kulakisiz, R., & Çevik, M. (2010). The effect of antioxidants on post-thawed Angora goat (Capra hircus ancryrensis) sperm parameters, lipid peroxidation and antioxidant activities. Small Ruminant Research, 89(1), 24-30. doi: 10.1016/j. smallrumres.2009.11.015
Budai, C., Egerszegi, I., Olah, J., Javor, A., & Kovacs, A. (2014). The protective effect of antioxidants on liquid and frozen stored ram semen. Scientific Papers Animal Science and Biotechnologies, 47(1), 46-52.
Delmas, D., Jannin, B., & Latruffe, N. (2005). Resveratrol: preventing properties against vascular alterations and ageing. Molecular Nutrition & Food Research, 49(5), 377-395. doi: 10.1002/mnfr.200400098
El-Khawagah, A. R. M., Kandiel, M. M. M., & Samir, H. (2020). Effect of quercetin supplementation in extender on sperm kinematics, extracellular enzymes release, and oxidative stress of Egyptian buffalo bulls frozen thawed semen. Frontiers in Veterinary Science, 7. doi: 10.3389/fvets.2020.604460
Fidan, A., Yeni, D., Avdatek, F., Özçinar, U., & Hazman, O. (2018). Changes in oxidative stress markers during electro-ejaculation procedure in merino rams. Jornal of the Hellenic Veterinary Medical Society, 68(3), 479-486. doi: 10.12681/jhvms.15544
Gee, G. F., Baskt, M. R., & Sexton, S. F. (1985). Cryogenic preservation of semen from the greater sandhill crane. Jornal of Wildlife Management, 49(2), 480-484.
Gibb, Z., Butler, T. J., Morris, L. H. A., Maxwell, W. M. C., & Grupen, C. G. (2013). Quercetin improves the postthaw characteristics of cryopreserved sex-sorted and nonsorted stallion sperm. Theriogenology, 79(6), 1001-1009. doi: 10.1016/j.theriogenology.2012.06.032
Guardia, T., Rotelli, A. E., Juarez, A. O., & Pelzer, L. E. (2001). Anti-inflammatory properties of plant flavonoids. Effects of rutin, quercetin and hesperidin on adjuvant arthritis in rat. Farmaco, 56(9), 683-687. doi: 10.1016/S0014-827X(01)01111-9
Gurtovenko, A. A., & Anwar, J. (2007). Modulating the structure and properties of cell membranes: the molecular mechanism of action of dimethyl sulfoxide. Journal of Physical Chemistry Biochem, 111(35), 10453e60. doi: 10.1021/jp073113e
Guthrie, H. D., & Welch, G. R. (2012). Effects of reactive oxygen species on sperm function. Theriogenology, 78(8), 1700-1708. doi: 10.1016/j.theriogenology.2012.05.002
Harrison, R. A. P., & Vickers, S. E. (1990). Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa. Journal of Reproduction and Fertility, 88(1), 343-352. doi: 10.1530/jrf.0.088 0343
Herrera, J. A., Quintana, J. A., Lopez, M. A., Betancourt, M., & Fierro, R. (2005). Individual cryo-preservation with dimethyl sulfoxide and polyvinylpyrrolidone of ejaculates and pooled semen of three avian species. Archives of Andrology, 51(5), 353-360. doi: 10.1080/014850190944401
Holt, W. V. (1997). Alternative strategies for the long-term preservation of spermatozoa. Reproduction, Fertility and Development, 9(3), 309-319. doi: 10.1071/R96082
Iaffaldano, N., Di Iorio, M., Miranda, M., Zaniboni, L., Manchisi, A., & Cerolini, S. (2016). Cryopreserving Turkey semen in straws and nitrogen vapour using DMSO or DMA: effects of cryoprotectant concentration, freezing rate and thawing rate on post-thaw semen quality. British Poultry Science, 57(2), 264-270. doi: 10.1080/00071668.2016.1148261
Ijaz, A., Hussain, A., Aleem, M., Yousaf, M. S., & Rehman, H. (2009). Butylated hydroxytoluene inclusion in semen extender improves the post-thawed semen quality of Nili-Ravi buffalo (Bubalus bubalis). Theriogenology, 71(8), 1326-1329. doi: 10.1016/j.theriogenology.2008.12.023
Jara, B., Merino, O., Sánchez, R., & Risopatrón, J. (2019). Positive effect of butylated hydroxytoluene (BHT) on the quality of cryopreserved cat spermatozoa. Cryobiology, 89, 76-81. doi: 10.1016/j. cryobiol.2019.05.003
Johinke, D., & Bathgate, R. (2014). Quercetin reduces the in vitro production of H2O2 during chilled storage of rabbit spermatozoa. Animal Reproduction Science, 151(3-4), 208-219. doi: 10.1016/j.anireprosci. 2014.10.017
Kawasaki, Y., Sakurai, D., Yoshihara, T., Tsuchida, M., Harakawa, S., & Suzuki, H. (2020). Effect of quercetin on the motility of cryopreserved canine spermatozoa. Cryobiology, 96, 50-54. doi: 10.1016/j. cryobiol.2020.08.006
Li, Y. (2011). Antioxidants in biology and medicine: essentials, advances and clinical applications. Blacksburg (Virgínia): Nova Science Publishers.
Mandiki, S. N. M., Derycke, G., Bister, J. L., & Paquay, R. (1998). Influence of season and age on sexual maturation parameters of Texel, Suffolk and Ile-de-France rams 1. Testicular size, semen quality and reproductive capacity. Small Ruminant Research, 28(1), 67-79. doi: 10.1016/S0921-4488(97)00073-4
Memon, A. A., Wahid, H., Rosnina, Y., Goh, Y. M., Ebrahimi, M., Nadia, F. M., & Audrey, G. (2011). Effect of butylated hydroxytoluene on cryopreservation of Boer goat semen in Tris egg yolk extender. Animal Reproduction Science, 129(1-2), 44-49. doi: 10.1016/j.anireprosci.2011.10.004
Merino, O., Aguaguina, W. E., Esponda, P., Risopatrón, J., Isachenko, E., Isachenko, V., & Sánchez, R. (2015). Protective effect of butylated hydroxytoluene on sperm function in human spermatozoa cryopreserved by vitrification technique. Andrologia, 47(2), 186-193. doi: 10.1111/and.12246
Neagu, V. R., García, B. M., Sandoval, C. S., Rodríguez, A. M., Ferrusola, C. O., Fernández L. G.,… Peña, F. J. (2010). Freezing dog semen in presence of the antioxidant butylated hydroxytoluene improves postthaw sperm membrane integrity. Theriogenology, 73(5), 645-650. doi: 10.1016/j.theriogenology. 2009.10.021
Nitiema, L. W., Savadogo, A., Simpore, J., Dianou, D., & Traore, A. S. (2012). In vitro antimicrobial activity of some phenolic compounds (coumarin and quercetin) against gastroenteritis bacterial strains. International Journal of Microbiological Research, 3(3), 183-187. doi: 10.5829/idosi.ijmr.2012.3.3
Oliveira, M. A.D., Oliveira, R., Lima, A., Andrade, E., Abreu, J., & Oliveira, F. (2017). Physical evaluation, morphological and identification of seminal proteins in Santa Ines sheep. Revista Brasileira Saúde e Produção Animal, 18(1), 211-220. doi: 10.1590/S1519-99402017000100020
Palomo, M. J., García, W., & Tabarez, G. (2017). Effect of seminal plasma and butylated hydroxytoluene (BHT) concentration on ram sperm freezability. Small Ruminant Research, 153, 66-70. doi: 10.1016/j. smallrumres.2017.05.010
Parks, J. E., & Lynch, D. V. (1992). Lipid composition and thermotropic phase behavior of boar, bull, stallion, and rooster sperm membranes. Cryobiology, 29(2), 255-266. doi: 10.1016/0011-2240(92)900 24-V
Rakha, B. A., Ansari, M. S., Akhter, S., Zafar, Z., Naseer A., Hussain, I., Santiago-Moreno, J. (2018). Use of dimethylsulfoxide for semen cryopreservation in Indian red jungle fowl (Gallus gallus murghi). Theriogenology, 122, 61-67. doi: 10.1016/j.theriogenology.2018.09.003
Salamon, S., & Maxwell, W. M. C. (2000). Storage of ram semen. Animal Reproduction Science, 62(1-3), 77-111. doi: 10.1016/S0378-4320(00)00155-X
Seifi-Jamadi, A., Kohram, H., Zareh-Shahne, A., Dehghanizadeh, P., & Ahmad, E. (2016). Effect of various concentrations of butylated hydroxyanisole and butylated hydroxytoluene on freezing capacity of Turkman stallion sperm. Animal Reproduction Science, 170(july), 108-113. doi: 10.1016/j.anireprosci. 2016.04.010
Shoae, A., & Zamiri, M. J. (2008). Effect of butylated hydroxytoluene on bull spermatozoa frozen in egg yolk-citrate extender. Animal Reproduction Science, 104(2-4), 414-418. doi: 10.1016/j.anireprosci.2007 .07.009
Sikka, S. C. (2004). Role of oxidative stress and antioxidants in andrology and assisted reproductive technology. Journal of Andrology, 25(1), 5-18. doi: 10.1002/j.1939-4640.2004.tb02751.x
Silva, E. C. B., Cajueiro, J. F. P., Silva, S. V., Soares, P. C., & Guerra, M. M. P. (2012). Effect of antioxidants resveratrol and quercetin on in vitro evaluation of frozen ram sperm. Theriogenology, 77(8), 1722-1726. doi: 10.1016/j.theriogenology.2011.11.023
Sun, L., Wu, C., Xu, J., Zhang, S., Dai, J., & Zhang, D. (2020). Addition of butylated hydroxytoluene (BHT) in tris-based extender improves post-thaw quality and motion dynamics of dog spermatozoa. Cryobiology, 97, 71-75. doi: 10.1016/j.cryobiol.2020.10.006
Trzcinska, M., Bryla, M., Gadja, b., & Gogol, P. (2015). Fertility of boar sêmen cryopreserved in extender supplemented with butylated hydroxytoluene. Theriogenology, 83(3), 307-313. doi: 10.1016/j.theriogenology.2014.07.045
Tvrdá, E., Tusimová, E., Kovacik, A., Paál, D., & Lukac, N. (2016). Protective effects of quercetin on selected oxidative biomarkers in bovine spermatozoa subjected to ferrous ascorbate. Reproduction in Domestic Animals, 51(4), 524-537. doi: 10.1111/rda.12714
Watson, P. F., & Anderson, W. J. (1983). Influence of butylated hydroxytoluene (BHT) on the viability of ram spermatozoa undergoing cold shock. Journal of Reproduction and Fertility, 69(1), 229-235. doi: 10.1530/jrf.0.0690229
Amidi, F., Pazhohan, A., Shabani, N. M., Khodarahmian, M., & Nekoonam, S. (2016). The role of antioxidants in sperm freezing: a review. Cell and Tissue Banking, 17(4), 745-756. doi: 10.1007/s10561 -016-9566-5
Appiah, M. O., Li, W., Zhao, J., Liu, H., Dong, Y., Xiang, J.,… Lu, W. (2020). Quercetin supplemented casein-based extender improves the post-thaw quality of rooster semen. Cryobiology, 94, 57-65. doi: 10. 1016/j.cryobiol.2020.04.010
Avdatek, F., Yeni, D., Inanc, M. E., Çil, B., Tuncer, B. P., Turkmen, R., & Tasdemir, U. (2018). Supplementation of quercetin for advanced DNA integrity in bull semen cryopreservation. Andrologia, 50(4), 1-7. doi: 10.1111/and.12975
Banday, M. N., Lone, F. A., Rasool, F., Rashid, M., & Shikari, A. (2017). Use of antioxidants reduce lipid peroxidation and improve quality of crossbred ram sperm during its cryopreservation. Cryobiology, 74, 25-30. doi: 10.1016/j.cryobiol.2016.12.008
Ben, A. F., Zribi, N., & Ammar-Keskes, L. (2011). Antioxidative potential of Quercetin against hydrogen peroxide induced oxidative stress in spermatozoa in vitro. Andrologia, 43(4), 261-265. doi: 10.1111/j. 1439-0272.2010.01063.x
Boots, A. W., Haenen, G. R. M. M., & Bast, A. (2008). Health effects of quercetin: from antioxidant to nutraceutical. European Journal of Pharmacology, 585(2-3), 325-337. doi: 10.1016/j.ejphar.2008.03.0 08
Brito, L. F. C. (2007). Evaluation of stallion sperm morphology. Clinical Techiniques in Equine Practice, 6(4), 249-264. doi: 10.1053/j.ctep.2007.09.004
Bucak, M. N., Sariozkan, S., Tuncer, P. B., Sakin, F., Atessahin, A., Kulakisiz, R., & Çevik, M. (2010). The effect of antioxidants on post-thawed Angora goat (Capra hircus ancryrensis) sperm parameters, lipid peroxidation and antioxidant activities. Small Ruminant Research, 89(1), 24-30. doi: 10.1016/j. smallrumres.2009.11.015
Budai, C., Egerszegi, I., Olah, J., Javor, A., & Kovacs, A. (2014). The protective effect of antioxidants on liquid and frozen stored ram semen. Scientific Papers Animal Science and Biotechnologies, 47(1), 46-52.
Delmas, D., Jannin, B., & Latruffe, N. (2005). Resveratrol: preventing properties against vascular alterations and ageing. Molecular Nutrition & Food Research, 49(5), 377-395. doi: 10.1002/mnfr.200400098
El-Khawagah, A. R. M., Kandiel, M. M. M., & Samir, H. (2020). Effect of quercetin supplementation in extender on sperm kinematics, extracellular enzymes release, and oxidative stress of Egyptian buffalo bulls frozen thawed semen. Frontiers in Veterinary Science, 7. doi: 10.3389/fvets.2020.604460
Fidan, A., Yeni, D., Avdatek, F., Özçinar, U., & Hazman, O. (2018). Changes in oxidative stress markers during electro-ejaculation procedure in merino rams. Jornal of the Hellenic Veterinary Medical Society, 68(3), 479-486. doi: 10.12681/jhvms.15544
Gee, G. F., Baskt, M. R., & Sexton, S. F. (1985). Cryogenic preservation of semen from the greater sandhill crane. Jornal of Wildlife Management, 49(2), 480-484.
Gibb, Z., Butler, T. J., Morris, L. H. A., Maxwell, W. M. C., & Grupen, C. G. (2013). Quercetin improves the postthaw characteristics of cryopreserved sex-sorted and nonsorted stallion sperm. Theriogenology, 79(6), 1001-1009. doi: 10.1016/j.theriogenology.2012.06.032
Guardia, T., Rotelli, A. E., Juarez, A. O., & Pelzer, L. E. (2001). Anti-inflammatory properties of plant flavonoids. Effects of rutin, quercetin and hesperidin on adjuvant arthritis in rat. Farmaco, 56(9), 683-687. doi: 10.1016/S0014-827X(01)01111-9
Gurtovenko, A. A., & Anwar, J. (2007). Modulating the structure and properties of cell membranes: the molecular mechanism of action of dimethyl sulfoxide. Journal of Physical Chemistry Biochem, 111(35), 10453e60. doi: 10.1021/jp073113e
Guthrie, H. D., & Welch, G. R. (2012). Effects of reactive oxygen species on sperm function. Theriogenology, 78(8), 1700-1708. doi: 10.1016/j.theriogenology.2012.05.002
Harrison, R. A. P., & Vickers, S. E. (1990). Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa. Journal of Reproduction and Fertility, 88(1), 343-352. doi: 10.1530/jrf.0.088 0343
Herrera, J. A., Quintana, J. A., Lopez, M. A., Betancourt, M., & Fierro, R. (2005). Individual cryo-preservation with dimethyl sulfoxide and polyvinylpyrrolidone of ejaculates and pooled semen of three avian species. Archives of Andrology, 51(5), 353-360. doi: 10.1080/014850190944401
Holt, W. V. (1997). Alternative strategies for the long-term preservation of spermatozoa. Reproduction, Fertility and Development, 9(3), 309-319. doi: 10.1071/R96082
Iaffaldano, N., Di Iorio, M., Miranda, M., Zaniboni, L., Manchisi, A., & Cerolini, S. (2016). Cryopreserving Turkey semen in straws and nitrogen vapour using DMSO or DMA: effects of cryoprotectant concentration, freezing rate and thawing rate on post-thaw semen quality. British Poultry Science, 57(2), 264-270. doi: 10.1080/00071668.2016.1148261
Ijaz, A., Hussain, A., Aleem, M., Yousaf, M. S., & Rehman, H. (2009). Butylated hydroxytoluene inclusion in semen extender improves the post-thawed semen quality of Nili-Ravi buffalo (Bubalus bubalis). Theriogenology, 71(8), 1326-1329. doi: 10.1016/j.theriogenology.2008.12.023
Jara, B., Merino, O., Sánchez, R., & Risopatrón, J. (2019). Positive effect of butylated hydroxytoluene (BHT) on the quality of cryopreserved cat spermatozoa. Cryobiology, 89, 76-81. doi: 10.1016/j. cryobiol.2019.05.003
Johinke, D., & Bathgate, R. (2014). Quercetin reduces the in vitro production of H2O2 during chilled storage of rabbit spermatozoa. Animal Reproduction Science, 151(3-4), 208-219. doi: 10.1016/j.anireprosci. 2014.10.017
Kawasaki, Y., Sakurai, D., Yoshihara, T., Tsuchida, M., Harakawa, S., & Suzuki, H. (2020). Effect of quercetin on the motility of cryopreserved canine spermatozoa. Cryobiology, 96, 50-54. doi: 10.1016/j. cryobiol.2020.08.006
Li, Y. (2011). Antioxidants in biology and medicine: essentials, advances and clinical applications. Blacksburg (Virgínia): Nova Science Publishers.
Mandiki, S. N. M., Derycke, G., Bister, J. L., & Paquay, R. (1998). Influence of season and age on sexual maturation parameters of Texel, Suffolk and Ile-de-France rams 1. Testicular size, semen quality and reproductive capacity. Small Ruminant Research, 28(1), 67-79. doi: 10.1016/S0921-4488(97)00073-4
Memon, A. A., Wahid, H., Rosnina, Y., Goh, Y. M., Ebrahimi, M., Nadia, F. M., & Audrey, G. (2011). Effect of butylated hydroxytoluene on cryopreservation of Boer goat semen in Tris egg yolk extender. Animal Reproduction Science, 129(1-2), 44-49. doi: 10.1016/j.anireprosci.2011.10.004
Merino, O., Aguaguina, W. E., Esponda, P., Risopatrón, J., Isachenko, E., Isachenko, V., & Sánchez, R. (2015). Protective effect of butylated hydroxytoluene on sperm function in human spermatozoa cryopreserved by vitrification technique. Andrologia, 47(2), 186-193. doi: 10.1111/and.12246
Neagu, V. R., García, B. M., Sandoval, C. S., Rodríguez, A. M., Ferrusola, C. O., Fernández L. G.,… Peña, F. J. (2010). Freezing dog semen in presence of the antioxidant butylated hydroxytoluene improves postthaw sperm membrane integrity. Theriogenology, 73(5), 645-650. doi: 10.1016/j.theriogenology. 2009.10.021
Nitiema, L. W., Savadogo, A., Simpore, J., Dianou, D., & Traore, A. S. (2012). In vitro antimicrobial activity of some phenolic compounds (coumarin and quercetin) against gastroenteritis bacterial strains. International Journal of Microbiological Research, 3(3), 183-187. doi: 10.5829/idosi.ijmr.2012.3.3
Oliveira, M. A.D., Oliveira, R., Lima, A., Andrade, E., Abreu, J., & Oliveira, F. (2017). Physical evaluation, morphological and identification of seminal proteins in Santa Ines sheep. Revista Brasileira Saúde e Produção Animal, 18(1), 211-220. doi: 10.1590/S1519-99402017000100020
Palomo, M. J., García, W., & Tabarez, G. (2017). Effect of seminal plasma and butylated hydroxytoluene (BHT) concentration on ram sperm freezability. Small Ruminant Research, 153, 66-70. doi: 10.1016/j. smallrumres.2017.05.010
Parks, J. E., & Lynch, D. V. (1992). Lipid composition and thermotropic phase behavior of boar, bull, stallion, and rooster sperm membranes. Cryobiology, 29(2), 255-266. doi: 10.1016/0011-2240(92)900 24-V
Rakha, B. A., Ansari, M. S., Akhter, S., Zafar, Z., Naseer A., Hussain, I., Santiago-Moreno, J. (2018). Use of dimethylsulfoxide for semen cryopreservation in Indian red jungle fowl (Gallus gallus murghi). Theriogenology, 122, 61-67. doi: 10.1016/j.theriogenology.2018.09.003
Salamon, S., & Maxwell, W. M. C. (2000). Storage of ram semen. Animal Reproduction Science, 62(1-3), 77-111. doi: 10.1016/S0378-4320(00)00155-X
Seifi-Jamadi, A., Kohram, H., Zareh-Shahne, A., Dehghanizadeh, P., & Ahmad, E. (2016). Effect of various concentrations of butylated hydroxyanisole and butylated hydroxytoluene on freezing capacity of Turkman stallion sperm. Animal Reproduction Science, 170(july), 108-113. doi: 10.1016/j.anireprosci. 2016.04.010
Shoae, A., & Zamiri, M. J. (2008). Effect of butylated hydroxytoluene on bull spermatozoa frozen in egg yolk-citrate extender. Animal Reproduction Science, 104(2-4), 414-418. doi: 10.1016/j.anireprosci.2007 .07.009
Sikka, S. C. (2004). Role of oxidative stress and antioxidants in andrology and assisted reproductive technology. Journal of Andrology, 25(1), 5-18. doi: 10.1002/j.1939-4640.2004.tb02751.x
Silva, E. C. B., Cajueiro, J. F. P., Silva, S. V., Soares, P. C., & Guerra, M. M. P. (2012). Effect of antioxidants resveratrol and quercetin on in vitro evaluation of frozen ram sperm. Theriogenology, 77(8), 1722-1726. doi: 10.1016/j.theriogenology.2011.11.023
Sun, L., Wu, C., Xu, J., Zhang, S., Dai, J., & Zhang, D. (2020). Addition of butylated hydroxytoluene (BHT) in tris-based extender improves post-thaw quality and motion dynamics of dog spermatozoa. Cryobiology, 97, 71-75. doi: 10.1016/j.cryobiol.2020.10.006
Trzcinska, M., Bryla, M., Gadja, b., & Gogol, P. (2015). Fertility of boar sêmen cryopreserved in extender supplemented with butylated hydroxytoluene. Theriogenology, 83(3), 307-313. doi: 10.1016/j.theriogenology.2014.07.045
Tvrdá, E., Tusimová, E., Kovacik, A., Paál, D., & Lukac, N. (2016). Protective effects of quercetin on selected oxidative biomarkers in bovine spermatozoa subjected to ferrous ascorbate. Reproduction in Domestic Animals, 51(4), 524-537. doi: 10.1111/rda.12714
Watson, P. F., & Anderson, W. J. (1983). Influence of butylated hydroxytoluene (BHT) on the viability of ram spermatozoa undergoing cold shock. Journal of Reproduction and Fertility, 69(1), 229-235. doi: 10.1530/jrf.0.0690229
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2022-02-25
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Canuto, L. E. F., Segabinazzi, L. G. T. M., Araújo, E. A. B. de, Silva, L. F. M. C., Oliveira, S. N. de, Dalanezi, F. M., … Oba, E. (2022). Efeito da inclusão de quercetina ou butil-hidroxitolueno na qualidade do sêmen de carneiro resfriado ou congelado. Semina: Ciências Agrárias, 43(2), 841–854. https://doi.org/10.5433/1679-0359.2022v43n2p841
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