Molecular survey of Cryptosporidium spp. in calves from the state of Mato Grosso, Brazil
DOI:
https://doi.org/10.5433/1679-0359.2020v41n5supl1p2437Keywords:
Calf, Cryptosporidium parvum, Feces, Zoonosis.Abstract
Cryptosporidium spp. is a protozoan that infects a wide range of vertebrate hosts; it has been reported to be the cause of severe illness or death in livestock worldwide, which leads to decreased performance and production losses, especially in young animals. This study investigated the presence of Cryptosporidium in calves from beef farms in the state of Mato Grosso, midwestern Brazil. For this purpose, fecal samples from 237 animals aged ? 45 days, raised in 20 rural properties were subjected to DNA extraction and nested polymerase chain reaction (nPCR) targeting 18S ribosomal RNA (18S rRNA) gene followed by sequencing. Additionally, positive samples, previously identified as Cryptosporidium parvum by sequencing and phylogenetic analyses based on 18S rRNA gene, were subsequently analyzed focusing the amplification and sequencing using nPCR of a fragment of the 60 kDa glycoprotein (gp60) gene. Of the 237 fecal samples analyzed by PCR (18S rRNA), 50 (21.1%) fecal samples were positive for Cryptosporidium spp., while 14 (70%) of the 20 properties had at least one positive animal. The following Cryptosporidium species were detected: C. bovis, C. parvum, and C. ryanae. Thereafter, two potentially zoonotic subtypes (IIaA15G2R1 and IIaA16G3R1) of C. parvum were identified based on gp60 gene sequences. This study resulted in the detection of subtype IIaA16G3R1 for the first time in South America and showed a wide distribution of the protozoan in beef farms in the studied area of the State.Downloads
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References
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Trotz-Williams, L. A., Martin, D. S., Gatei, W., Cama, V., Peregrine, A. S., Martin, S. W.,... Xiao, L. (2006). Genotype and subtype analyses of Cryptosporidium isolates from dairy calves and humans in Ontario. Parasitology Research, 99(4), 346-352. doi: 10.1007/s00436-006-0157-4
Xiao, L. (2010). Molecular epidemiology of cryptosporidiosis: an update. Experimental Parasitology, 124(1), 80-89. doi: 10.1016/j.exppara.2009.03.018
Xiao, L., Morgan, U. M., Limor, J., Escalante, A., Arrowood, M., Shulaw, W.,… Lal, A. A. (1999). Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. Applied Environment Microbiology, 65(8), 3386-3391.
Cavalier-Smith, T. (2014). Gregarine site-heterogeneous 18S rDNA trees, revision of gregarine higher classification, and the evolutionary diversification of Sporozoa. European Journal of Protistology, 50(5), 472-495. doi: 10.1016/j.ejop.2014.07.002
Holsback, L., Lima, H. E., Vidotto, O., Silva, M. A. D., Patelli, T. H. C., & Martins, F. D. C. (2018). Cryptosporidium occurrence in ruminants from the North Pioneer mesoregion of Paraná, Brazil. Revista Brasileira de Parasitologia Veterinária, 27(2), 248-253. doi: 10.1590/S1984-296120180037
Kimura, M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16(2), 111-120. doi: 10. 1007/BF01731581
McAllister, T. A., Olson, M. E., Fletch, A., Wetzstein, M., & Entz, T. (2005). Prevalence of Giardia and Cryptosporidium in beef cows in southern Ontario and in beef calves in southern British Columbia. Canadian Veterinary Journal, 46(1), 47-55. doi: 10.4141/cjas66-008
Oliveira, J. P., F., Silva, D. P. G., Pacheco, M. D., Mascarini, L. M., Ribeiro, M. G., Alfieri, A. A. Borges, A.,... S. (2007). Diarréia em bezerros da raça Nelore criados extensivamente: estudo clínico e etiológico. Pesquisa Veterinária Brasileira, 27(10), 419-424. doi: 10.1590/S0100-736X2007001000006
Razakandrainibe, R., Diawara, E. H. I., Costa, D., Le Goff, L., Lemeteil, D., Ballet, J. J.,... Favennec, L. (2018). Common occurrence of Cryptosporidium hominis in asymptomatic and symptomatic calves in France. PLoS Neglected Tropical Diseases, 12(3), e0006355. doi: 10.1371/journal.pntd.0006355
Rieux, A., Paraud, C., Pors, I., & Chartier, C. (2014). Molecular characterization of Cryptosporidium isolates from beef calves under one month of age over three successive years in one herd in western France. Veterinary Parasitology, 202(3-4), 171-179. doi: 10.1016/j.vetpar.2014.03.004
Robertson, L. J., Björkman, C., Axén, C., & Fayer, R. (2014). Cryptosporidium: parasite and disease. In S. M. Caccio, & G. Widmer (Eds.), Cryptosporidium: parasite and disease (pp. 149-235). Wien: Springer-Verlag.
Silverlås, C., Bosaeus-Reineck, H., Näslund, K., & Björkman, C. (2013). Is there a need for improved Cryptosporidium diagnostics in Swedish calves? International Journal of Parasitology, 43(2), 155-161. doi: 10.1016/j.ijpara.2012.10.009
Silverlås, C., de Verdier, K., Emanuelson, U., Mattsson, J. G., & Björkman, C. (2010). Cryptosporidium infection in herds with and without calf diarrheal problems. Parasitology Research, 107(6), 1435-1444. doi: 10.1007/s00436-010-2020-x
Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: molecular evolution-ary genetics analysis version 6.0. Molecular Biology and Evolution, 30(12), 2725-2729. doi: 10.1093/ molbev/mst197
Toledo, R. D., Martins, F. D., Ferreira, F. P., Almeida, J. C., Ogawa, L., Santos, H. L. dos,... Freire, R. L. (2017). Cryptosporidium spp. and Giardia spp. in feces and water and the associated exposure factors on dairy farms. PLoS One, 12(4), e0175311. doi: 10.1371/journal.pone.0175311
Trotz-Williams, L. A., Martin, D. S., Gatei, W., Cama, V., Peregrine, A. S., Martin, S. W.,... Xiao, L. (2006). Genotype and subtype analyses of Cryptosporidium isolates from dairy calves and humans in Ontario. Parasitology Research, 99(4), 346-352. doi: 10.1007/s00436-006-0157-4
Xiao, L. (2010). Molecular epidemiology of cryptosporidiosis: an update. Experimental Parasitology, 124(1), 80-89. doi: 10.1016/j.exppara.2009.03.018
Xiao, L., Morgan, U. M., Limor, J., Escalante, A., Arrowood, M., Shulaw, W.,… Lal, A. A. (1999). Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. Applied Environment Microbiology, 65(8), 3386-3391.
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Published
2020-08-07
How to Cite
Pacheco, T. dos A., Martins, F. D. C., Almeida, S. L. H. de, Semedo, T. B. F., Watanabe, M. I., Dutra, V., … Pacheco, R. C. (2020). Molecular survey of Cryptosporidium spp. in calves from the state of Mato Grosso, Brazil. Semina: Ciências Agrárias, 41(5supl1), 2437–2444. https://doi.org/10.5433/1679-0359.2020v41n5supl1p2437
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