Occurrence of gastrointestinal protozoans in cats from Londrina, Paraná, Brazil





PCR. Feline, Shelter, Cryptosporidium muris, Toxoplasma gondii, Giardia duodenalis.


Protozoans are widely distributed, and several species may parasitize the digestive tracts of cats (Felis catus), and can be transmitted to humans. The present study aimed to evaluate the species and occurrence of gastrointestinal protozoans in cats in the city of Londrina, Paraná, Brazil. A total of 206 cat fecal samples were tested, of which 141 were from shelter animals, and 65 were from pets owned by local people. Samples were processed by parasitological techniques. Coproparasitological techniques (Willis, Faust and Ziehl-Neelsen) were performed for detection of protozoan parasites. Subsequently, all samples were processed by PCR protocols specific to Toxoplasma gondii, Giardia spp., and Cryptosporidium spp. PCR products from positive samples were selected for sequencing. No samples were found to be positive for Cryptosporidium spp. using the Ziehl-Neelsen technique. Using specific PCR protocols, 1/206 (0.48%) samples tested positive for Cryptosporidium spp. After purification, this one positive sample was sequenced, and it demonstrated a 100% identity match to Cryptosporidium muris. Using specific PCR protocols, 13/206 (9.22%) cat fecal samples tested, including 2/65 (3.08%) pet cat fecal samples, were positive for T. gondii. PCR analysis revealed that 37/206 (17.96%) of cat fecal samples were positive for Giardia spp., including 27/141 (19.15%) of shelter cat fecal samples, and 10/65 (15.38%) pet cat fecal samples (p = 0.5124). When sequenced, these positive samples showed a 100% identity match with Giardia duodenalis. This study demonstrated that infections with Cryptosporidium spp., Toxoplasma gondii, and Giardia duodenalis are present in the population of both pet cats and shelter cats in the city of Londrina. This poses a risk to public health, because these parasites have a high zoonotic potential.

Biografia do Autor

Keila Jimenez Torrico, Universidade Estadual de Londrina

Discente de Mestrado, Programa de Pós-Graduação em Ciência Animal, Universidade Estadual de Londrina, UEL, Londrina, PR, Brasil.

Nelson Jessé Rodrigues dos Santos, Universidade Estadual de Londrina

Discente de Doutorado, Programa de Pós-Graduação em Ciência Animal, UEL, Londrina, PR, Brasil.

Hugo Luca Abate, Universidade Estadual de Londrina

Discente de Doutorado, Programa de Pós-Graduação em Ciência Animal, UEL, Londrina, PR, Brasil.

Felippe Danyel Cardoso Martins, Universidade Estadual de Londrina

Discente de Doutorado, Programa de Pós-Graduação em Ciência Animal, UEL, Londrina, PR, Brasil.

Luiz Daniel de Barros, Universidade Estadual de Londrina

Prof. Dr., Departamento de Medicina Veterinária, UEL, Londrina, PR, Brasil.

Mércia Seixas, Universidade Estadual de Londrina

Discente de Doutorado, Programa de Pós-Graduação em Ciência Animal, UEL, Londrina, PR, Brasil.

Thais Agostinho Martins, Universidade Estadual de Londrina

Discente de Doutorado, Programa de Pós-Graduação em Ciência Animal, UEL, Londrina, PR, Brasil.

João Luis Garcia, Universidade Estadual de Londrina

Prof. Dr., Departamento de Medicina Veterinária, UEL, Londrina, PR, Brasil.

Odilon Vidotto, Universidade Estadual de Londrina

Prof. Dr., Departamento de Medicina Veterinária, UEL, Londrina, PR, Brasil.


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Como Citar

Torrico, K. J., Santos, N. J. R. dos, Abate, H. L., Martins, F. D. C., Barros, L. D. de, Seixas, M., … Vidotto, O. (2020). Occurrence of gastrointestinal protozoans in cats from Londrina, Paraná, Brazil. Semina: Ciências Agrárias, 41(1), 213–222. https://doi.org/10.5433/1679-0359.2020v41n1p213




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