Effect of the antral follicle count of Bos taurus × Bos indicus dairy cows on in vitro embryo production

Authors

DOI:

https://doi.org/10.5433/1679-0359.2020v41n5supl1p2171

Keywords:

AFC, OPU., Embryo production, Bos taurus × Bos indicus.

Abstract

This study aimed to evaluate the potential of Girolando (Bos taurus × Bos indicus) cows with high and low antral follicle counts (AFC) for the in vitro production of bovine embryos (IVEP), as well as the pregnancy rates of the recipients of these embryos. Girolando cows (Bos taurus × Bos indicus) were classified as high and low AFC when they had 35-52 (n = 13) and 11-17 follicles (n = 15), respectively. All animals were subjected to repeated follicular aspiration [Ovum pick-up (OPU)] and subsequent IVEP sessions. The synchronization protocol of the recipients was performed on a random day of the estrous cycle (Day 0) with the implantation of progesterone, estradiol benzoate, and prostaglandin. The high AFC group had higher aspirated oocyte/OPU (42.6 ± 5.2 vs. 14.6 ± 1.9; p < 0.01) and cultured oocyte/OPU (38.1 ± 6.6 vs. 12.3 ± 2.8; p < 0.01) averages as well as a higher blastocyst percentage on D7 (23.0 ± 1.0% vs. 18.4 ± 1.5%; p < 0.05) and higher pregnancy rate (42.7 ± 2.7% vs. 39.7 ± 4.6%; p < 0.05) than the low AFC group. Thus, we can conclude that animals with high AFC had better IVEP success rates than animals with low AFC.

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Author Biographies

Sheila Merlo Garcia, Universidade do Oeste Paulista

Profa Dra, Universidade do Oeste Paulista, UNOESTE, Presidente Prudente, SP, Brasil.

Paula Alvares Lunardelli, Universidade Estadual de Londrina

Dra, Programa de Pós-Graduação em Ciência Animal, Universidade Estadual de Londrina, UEL, Londrina, PR, Brasil.

Kleber Luciano Ancioto, Gene Up

Gene Up, Presidente Prudente, SP, Brasil.

Eduardo Cardoso de Oliveira, Universidade Estadual Paulista Júlio de Mesquita Filho

M.e, Programa de Pós-Graduação em Matemática Aplicada e Computacional, Universidade Estadual Paulista “Júlio de Mesquita Filho”, UNESP, Presidente Prudente, SP, Brasil.

Larissa Zamparone Bergamo, Universidade Estadual de Londrina

Discente do Curso de Doutorado do Programa de Pós-Graduação em Ciência Animal, UEL, Londrina, PR, Brasil.

Amanda Fonseca Zangirolamo, Universidade Estadual de Londrina

Pós-Dra, Programa de Pós-Graduação em Ciência Animal, UEL, Londrina, PR, Brasil.

Marcelo Marcondes Seneda, Universidade Estadual de Londrina

Prof. Dr., Programa de Pós-Graduação em Ciência Animal, Departamento de Clínicas Veterinárias, UEL, Londrina, PR, Brasil.

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Published

2020-08-07

How to Cite

Garcia, S. M., Lunardelli, P. A., Ancioto, K. L., Oliveira, E. C. de, Bergamo, L. Z., Zangirolamo, A. F., & Seneda, M. M. (2020). Effect of the antral follicle count of Bos taurus × Bos indicus dairy cows on in vitro embryo production. Semina: Ciências Agrárias, 41(5supl1), 2171–2178. https://doi.org/10.5433/1679-0359.2020v41n5supl1p2171

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