Serological survey of porcine circovirus 2 antibodies in Northeastern Brazil by immunoperoxidase monolayer assay
DOI:
https://doi.org/10.5433/1679-0359.2020v41n1p345Keywords:
Circoviridae, Infection, Serum, Pig, Virus.Abstract
Porcine circovirus 2 (PCV2) specific antibodies were evaluated in pigs at 15 to 25 weeks of age by immunoperoxidase monolayer assay. Serum samples (n = 575) were obtained from 16 commercial farms located in six states of northeastern Brazil, including two in Ceará (n = 85), three in Rio Grande do Norte (n = 92), two in Paraíba (n = 49), three in Pernambuco (n = 107), two in Alagoas (n = 39), and four in Bahia (n = 203). Out of the 575 evaluated sera, 470 (81.74%) were reagents with antibody titers ranging from 20 to 5,120. All farms presented reactive animals. There was a statistically significant difference in reactivity proportions between the states of Bahia and Ceará (p < 0.05) and between Bahia and Rio Grande do Norte (p < 0.05). This study provides the first serological investigation for PCV2 in pigs from commercial farms in Northeastern Brazil.References
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Allan, G. M., Mcneilly, F., Kennedy, S., Daft, B., Clarke, E. G., Ellis, J. A.,... Adair, B. M. (1998). Isolation of porcine circovirus-like viruses from pigs with a wasting disease in the USA and Europe. Journal of Veterinary Diagnostic Investigation, 10(1), 3-10. doi: 10.1177/104063879801000102
Barbosa, C. N., Lobato, Z. I. P., Martins, N. R. S., Nascimento, E. F. (2008). Perfil sorológico para circovírus suíno tipo 2 em granjas comerciais de suínos no Brasil. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 60(4), 815-820. doi: 10.1590/s0102-09352008000400006
Barbosa, C. N., Martins, N. R. S., Esteves, E. G., Freitas, T. R. P. (2011). Pesquisa de antígenos e anticorpos contra Circovírus Suíno II em suínos com e sem sintomatologia da síndrome multisistêmica do definhamento em granjas comerciais mineiras. Semina: Ciências Agrárias, 32(4), 1513-1526. doi: 10.5433/1679-0359
Kim, J., Han, D. U., Choii, C., Chae, C. (2003). Simultaneous detection and differentiation between porcine circovirus and porcine parvovirus in boar semen by multiplex seminested polymerase chain reaction. Journal of Veterinary Medical Science, 65(6), 741-744. doi: 10.1292/jvms.65.741
Magar, R., Muller, P., Larochelle, R. (2000). Retrospective serological survey of antibodies to porcine circovirus type 1 and type 2. The Canadian Journal of Veterinary Research, 64(3), 184-186. PMCID: PMC1189611
Mesu, A. P., Labarque, G. G., Nauwynck, H. J., Pensaert, M. B. (2000). Seroprevalence of porcine circovirus types 1 and 2 in the Belgian pig population. The Veterinary Quarterly, 22(4), 234-236. doi: 10.1080/01652176.2000.9695065
Opriessnig, T., Meng, X. J., Halbur, P. G. (2007). Porcine circovirus type 2 associated disease: update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies. Journal of Veterinary Diagnostic Investigation, 19(6), 591-615. doi: 10.1177/104063870701900601
Opriessnig, T., Xiao, C. T., Halbur, P. G., Gerber, P. F., Matzinger, S. R., Meng, X. J. A. (2017). commercial porcine circovirus (PCV) type 2a-based vaccine reduces PCV2d viremia and shedding and prevents PCV2d transmission to naïve pigs under experimental conditions. Vaccine, 35(2), 248-254. doi: 10.1016/j.vaccine.2016.11.085
Pontes, N. E., Barbosa, C. N., Jesus, A. L. S., Silva, J. G., Freitas, A. C. (2012). Development and Evaluation of single-tube nested PCR (STNPCR) for the detection of porcine circovirus type 2 (PCV2). Transboundary and Emerging Diseases, 61(3), 233-238. doi: 10.1111/tbed.12022
Rodriguez-Arrioja, G. M., Segalés, J., Balasch, M., Rosell, C. J., Quintana, J., Folch, J. M., Domingo, M. (2000). Serum antibodies to porcine circovirus type 1 and type 2 in pigs with and without PMWS. Veterinary Record, 146(26), 762-764. doi: 10.1136/vr.146.26.762
Rose, N., Opriessnig, T., Grasland, B., Jestin, A. (2012). Epidemiology and transmission of porcine circovirus type 2 (PCV2). Virus Research, 164(1-2), 78-89. doi: 10.1016/j.virusres.2011.12.002
Sampaio, I. B. M. (2002). Estatística aplicada à experimentação animal. Belo Horizonte: Fundação de Estudo e Pesquisa em Medicina Veterinária e Zootécnica.
Tischer, I., Gelderblom, H., Vettermann, W., Koch, M. A. (1982). A very small porcine virus with circular single-stranded DNA. Nature, 295(5844), 64-65. doi:10.1038/295064a0
Zanella, J. R. C., Morés, N., Schiochet, M. F., Trombetta, C. (2001). Diagnóstico molecular e caracterização de circovírus suíno tipo 2 isolados no Brasil. Anais do Congresso Brasileiro de Veterinários Especialistas em Suínos, Porto Alegre, RS, Brasil, 11.
Zhou, J. Y., Chen, Q. X., Ye, J. X., Shen, H. G., Chen, T. F., Shang, S. B. (2006). Serological investigation and genomic characterization of PCV2 isolates from different geographic Regions of Zhejiang Province in China. Veterinary Research Communications, 30(2), 205-20. doi: 10.1007/s11259-006-3203-x
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2020-01-10
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Duque, P. R., Silva, A. S. A. da, Barros Júnior, M. R., Sergio, A. M. T., & Barbosa, C. N. (2020). Serological survey of porcine circovirus 2 antibodies in Northeastern Brazil by immunoperoxidase monolayer assay. Semina: Ciências Agrárias, 41(1), 345–350. https://doi.org/10.5433/1679-0359.2020v41n1p345
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