Incidence and transplacental transmission of Neospora caninum in primiparous females from Bos indicus slaughtered in Presidente Prudente, São Paulo, Brazil

Authors

  • Luis Carlos Vianna Universidade Estadual Paulista
  • Izidoro Francisco Sartor Universidade Estadual Paulista
  • Edviges Maristela Pituco Instituto Biológico
  • Liria Hiromi Okuda Instituto Biológico
  • Clarice Neves Camargo Instituto Biológico
  • Sergio do Nascimento Kronka Universidade do Oeste Paulista

DOI:

https://doi.org/10.5433/1679-0359.2008v29n2p387

Keywords:

Epidemiology, Cattle, Neospora caninum.

Abstract

To produce an epidemiological map of neosporosis in Brazil and identify the types of transmission of this disease, the present study evaluated the occurrence of Neospora caninum in Nelore cattle (Bos indicus) in Presidente Prudent, west region of Sao Paulo state; its vertical transmission; and the early stage in which fetuses are infected. To achieve this, serum samples from 518 slaughtered pregnant heifers and their fetuses were tested by ELISA technique and fetal brain tissues subjected to PCR. One hundred and three heifers (19.88%) had antibodies to N. caninum, as well as 38 (36.8%) of fetuses from 4 months of gestation. The conventional PCR failed to detect N. caninum DNA. These findings show that neosporosis occurs in the area studied and that it may be transmitted the transplacental route, althought N. caninum had not detected in brain tissue from non-aborted fetuses. The use of nested PCR it would be applied to increase the sensitivy of test.

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Author Biographies

Luis Carlos Vianna, Universidade Estadual Paulista

Medicina Veterinária, Pós-Graduação da Universidade Estadual Paulista, UNESP, Botucatu, SP, Brazil.CEP 18618-000.

Izidoro Francisco Sartor, Universidade Estadual Paulista

Medicina Veterinária, Universidade Estadual Paulista, UNESP, Botucatu, SP, Brazil.

Edviges Maristela Pituco, Instituto Biológico

Instituto Biológico, São Paulo, SP, Brazil.

Liria Hiromi Okuda, Instituto Biológico

Instituto Biológico, São Paulo, SP, Brazil.

Clarice Neves Camargo, Instituto Biológico

Instituto Biológico, São Paulo, SP, Brazil.

Sergio do Nascimento Kronka, Universidade do Oeste Paulista

Agronomia da Universidade do Oeste Paulista - UNOESTE, Presidente Prudente, SP, Brazil.

Published

2008-08-30

How to Cite

Vianna, L. C., Sartor, I. F., Pituco, E. M., Okuda, L. H., Camargo, C. N., & Kronka, S. do N. (2008). Incidence and transplacental transmission of Neospora caninum in primiparous females from Bos indicus slaughtered in Presidente Prudente, São Paulo, Brazil. Semina: Ciências Agrárias, 29(2), 387–392. https://doi.org/10.5433/1679-0359.2008v29n2p387

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