Comparison of the serologic tests of Indirect Immunofluorescence, Rapid Conglutination, Indirect ELISA and Competition ELISA for detection of antibodies against Anaplasma marginale in cattle sera from different enzootic areas
DOI:
https://doi.org/10.5433/1679-0359.2006v27n4p629Keywords:
Cattle, Anaplasma marginale, TCR, IFA, iELISA, cELISA.Abstract
The serological techniques Rapid Conglutination Test (RCT), Indirect ELISA (iELISA) and Indirect Immunofluorescent Assay (IFA), using the competition ELISA (cELISA) as gold test, were comparatively evaluated to detect antibodies against Anaplasma marginale. A total of 453 sera from vaccinated and non vaccinated cattle and, collected from enzootic stability and instability areas were tested. iELISA, IFA and TCR presented kappa index = 0.77 (substantial); 0.57 and 0.49 (moderate), sensibility of 90.6%; 90.2% and 73.7% and specificity of 86.6%; 62.8%, and 79.3%, respectively. Therefore, iELISA presented better specificity than IFA and RCT, and can be indicated for more detailed serological investigations, detection of persistently infected animals in cattle herds and for monitorating of vaccination programs. IFA and TCR can be used in prevalence studies and to monitor cattle movement between different geographical regions.
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