Molecular detection of canine distemper virus (CDV), canine adenovirus A type 1 and 2 (CAdV-1 and CAdV-2), and canine parvovirus type 2 (CPV-2) in the urine of naturally infected dogs

Autores

  • Ana Paula Silva Universidade Estadual de Londrina
  • Livia Bodnar Universidade Estadual de Londrina
  • Selwyn Arlington Headley Universidade Estadual de Londrina
  • Alice Fernandes Alfieri Universidade Estadual de Londrina
  • Amauri Alcindo Alfieri Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/1679-0359.2014v35n6p3231

Palavras-chave:

Babesiosis, Brazil, Diagnosis, ELISA, PCR.

Resumo

Bovine babesiosis is an important disease of cattle where Rhipicephalus microplus acts as a vector for the two causal organisms Babesia bovis and Babesia bigemina. A total of 22 calves were randomly monitored during three years by semi-nested PCR assay and ELISA test to determine prevalence of B. bovis and B. bigemina. The overall prevalence of B. bovis and B. bigemina was 30% and 35% by nested PCR (nPCR), and 70% and 75% by ELISA, respectively. Statistical analysis of the characteristics of animals showed that age and tick infestations (p<0.05) might play an important role in the spread of babesiosis as animal less than 6 months old. A high correlation (Kappa index of 0.70 for B. bovis and 0.65 for B. bigemina, respectively) between serological and molecular tests suggests that the combination of the utilized techniques in the present study is suitable for babesiosis diagnosis in an endemic unstable area.

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Biografia do Autor

Ana Paula Silva, Universidade Estadual de Londrina

Discente, Universidade Estadual de Londrina, UEL, Londrina, PR, Brasil.

Livia Bodnar, Universidade Estadual de Londrina

Discente, Università degli Studi di Bari Aldo Moro, UNIBA, Bari, Itália.

Selwyn Arlington Headley, Universidade Estadual de Londrina

Prof. Dr., UEL, Londrina, PR, Brasil.

Alice Fernandes Alfieri, Universidade Estadual de Londrina

Profª Drª, UEL, Londrina, PR, Brasil.

Amauri Alcindo Alfieri, Universidade Estadual de Londrina

Prof. Dr, UEL, Londrina, PR, Brasil.

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Publicado

2014-12-09

Como Citar

Silva, A. P., Bodnar, L., Headley, S. A., Alfieri, A. F., & Alfieri, A. A. (2014). Molecular detection of canine distemper virus (CDV), canine adenovirus A type 1 and 2 (CAdV-1 and CAdV-2), and canine parvovirus type 2 (CPV-2) in the urine of naturally infected dogs. Semina: Ciências Agrárias, 35(6), 3231–3236. https://doi.org/10.5433/1679-0359.2014v35n6p3231

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