Comparison of the serologic tests of Indirect Immunofluorescence, Rapid Conglutination, Indirect ELISA and Competition ELISA for detection of antibodies against Anaplasma marginale in cattle sera from different enzootic areas

Authors

  • Elizabete Regina Marangoni Marana Universidade Estadual de Londrina
  • Amauri A. Alfieri Universidade Estadual de Londrina
  • Gisele Maria de Andrade Universidade Estadual Paulista
  • Roberta Lemos Freire Universidade Estadual de Londrina
  • João Luís Garcia Universidade Estadual de Londrina
  • Odilon Vidotto Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/1679-0359.2006v27n4p629

Keywords:

Cattle, Anaplasma marginale, TCR, IFA, iELISA, cELISA.

Abstract

The serological techniques Rapid Conglutination Test (RCT), Indirect ELISA (iELISA) and Indirect Immunofluorescent Assay (IFA), using the competition ELISA (cELISA) as gold test, were comparatively evaluated to detect antibodies against Anaplasma marginale. A total of 453 sera from vaccinated and non vaccinated cattle and, collected from enzootic stability and instability areas were tested. iELISA, IFA and TCR presented kappa index = 0.77 (substantial); 0.57 and 0.49 (moderate), sensibility of 90.6%; 90.2% and 73.7% and specificity of 86.6%; 62.8%, and 79.3%, respectively. Therefore, iELISA presented better specificity than IFA and RCT, and can be indicated for more detailed serological investigations, detection of persistently infected animals in cattle herds and for monitorating of vaccination programs. IFA and TCR can be used in prevalence studies and to monitor cattle movement between different geographical regions.

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Author Biographies

Elizabete Regina Marangoni Marana, Universidade Estadual de Londrina

Médico Veterinário, Doutor, Técnico de Nível Superior, Laboratório de Protozoologia, Departamento de Medicina Veterinária Preventiva (DMVP), UEL, Londrina, PR.

Amauri A. Alfieri, Universidade Estadual de Londrina

Professor, Doutor, DMVP, UEL. Rodovia Celso Garcia Cid, PR 445, Km 380; Campus Universitário; Caixa Postal 6001. CEP:86051-990, Londrina, PR. Fone/Fax: 43 33714485.

Gisele Maria de Andrade, Universidade Estadual Paulista

Médico Veterinário, Doutor, Técnico de Nível Superior, UNESP-Jaboticabal, SP.

Roberta Lemos Freire, Universidade Estadual de Londrina

Professor, Doutor, DMVP, UEL. Rodovia Celso Garcia Cid, PR 445, Km 380; Campus Universitário; Caixa Postal 6001. CEP:86051-990, Londrina, PR. Fone/Fax: 43 33714485.

João Luís Garcia, Universidade Estadual de Londrina

Professor, Doutor, DMVP, UEL. Rodovia Celso Garcia Cid, PR 445, Km 380; Campus Universitário; Caixa Postal 6001. CEP:86051-990, Londrina, PR. Fone/Fax: 43 33714485.

Odilon Vidotto, Universidade Estadual de Londrina

Professor, Doutor, DMVP, UEL. Rodovia Celso Garcia Cid, PR 445, Km 380; Campus Universitário; Caixa Postal 6001. CEP:86051-990, Londrina, PR. Fone/Fax: 43 33714485.

Published

2006-07-29

How to Cite

Marana, E. R. M., Alfieri, A. A., Andrade, G. M. de, Freire, R. L., Garcia, J. L., & Vidotto, O. (2006). Comparison of the serologic tests of Indirect Immunofluorescence, Rapid Conglutination, Indirect ELISA and Competition ELISA for detection of antibodies against Anaplasma marginale in cattle sera from different enzootic areas. Semina: Ciências Agrárias, 27(4), 629–638. https://doi.org/10.5433/1679-0359.2006v27n4p629

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