Molecular diagnostic for a screening investigation method of tick-borne pathogens in Didelphis albiventris road-killed in north of Paraná, Brazil
DOI:
https://doi.org/10.5433/1679-0359.2023v44n1p451Palabras clave:
Ehrlichiosis, Opossum synanthropization.Resumen
The synanthropization of wild animals puts public health at risk by promoting the circulation of zoonotic agents, found naturally in the wild, in the anthropic environment. The objective of this work was to carry out screening by molecular detection of pathogens of the Anaplasmatacea family in Didelphis albiventris, a specie characterized as having a synanthropic habit. Opossums that were dead (n = 25) after being road-killed were collected in the North of Paraná state, southern Brazil during the 2016 and 2018 years, through active search. A questionnaire was filled out with information about the animal and collected place. Biological samples of spleen and liver were collected. The genetic material extracted from the spleen and liver was submitted to molecular diagnosis through PCR for amplification of dsb of Ehrlichia and 16S genes for the other agents of the Anaplasmataceae family. One animal was positive for the genus Ehrlichia in semi-nested PCR for amplification of the 349 bp fragment of the dsb gene in extracted from the liver samples. In PCR for the 16S target no animal was positive. These are preliminary results that reinforce the circulation of Ehrlichia in opossums. To improve the knowledge of these agents in opossums more studies are necessary.
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Almeida, A. P., Souza, T. D., Marcili, A., & Labruna, M. B. (2013). Novel Ehrlichia and Hepatozoon agents infecting the crab-eating fox (Cerdocyon thous) in southeastern Brazil. Journal of Medical Entomology, 50(3), 640-646. doi: 10.1603/ME12272 DOI: https://doi.org/10.1603/ME12272
André, M. R., Adania, C. H., Machado, R. Z., Allegretti, S. M., Felippe, P. A. N., Silva, K. F., & Nakaghi, A. C. H. (2010). Molecular and serologic detection of Ehrlichia spp. in endangered Brazilian wild captive felids. Journal of Wildlife Diseases, 46(3), 1017-1023. doi: 10.7589/0090-3558-46.3.1017 DOI: https://doi.org/10.7589/0090-3558-46.3.1017
Bezerra, M. C. F., Melo, A. L. T., Taques, I. I. G. G., Aguiar, D. M. D., Pacheco, R. C., & Slhessarenko, R. D. (2017). Seropositivity for Rickettsia spp. and Ehrlichia spp. in the human population of Mato Grosso, Central-Western Brazil. Revista da Sociedade Brasileira de Medicina Tropical, 50(3), 399-403. doi: 10.1590/0037-8682-0318-2016 DOI: https://doi.org/10.1590/0037-8682-0318-2016
Colle, A. C., Mendonça, R. F. B. D., Maia, M. O., Freitas, L. D. C., Witter, R., Marcili, A., Aguiar, D. M. de, Muñoz-Leal, S., Labruna, M. B., Rossi, R. V., & Pacheco, R. D. C. (2019). Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia. Revista Brasileira de Parasitologia Veterinária, 28(4), 592-604. doi: 10.1590/S1984-29612019086 DOI: https://doi.org/10.1590/s1984-29612019086
Doyle, C. K., Labruna, M. B., Breitschwerdt, E. B., Tang, Y. W., Corstvet, R. E., Hegarty, B. C., Bloch, K. C., Li, P., Walker, D. H., & McBride, J. W. (2005). Detection of medically important Ehrlichia by quantitative multicolor TaqMan real-time polymerase chain reaction of the dsb gene. The Journal of Molecular Diagnostics, 7(4), 504-510. doi: 10.1016/S1525-1578(10)60581-8 DOI: https://doi.org/10.1016/S1525-1578(10)60581-8
Ebani, V. V., Rocchigiani, G., Nardoni, S., Bertelloni, F., Vasta, V., Papini, R. A., Verin, R., Poli, A., & Mancianti, F. (2017). Molecular detection of tick-borne pathogens in wild red foxes (Vulpes vulpes) from Central Italy. Acta Tropica, 172(2017), 197-200. doi: 10.1016/j.actatropica.2017.05.014 DOI: https://doi.org/10.1016/j.actatropica.2017.05.014
Guimarães, A., Raimundo, J. M., Silva, A. T. D., Carpintero, F. M., Pires, J. R., Benevenute, J. L., Machado, R. Z., André, M. R., & Baldani, C. D. (2018). Detection of a putative novel genotype of Ehrlichia sp. from opossums (Didelphis aurita) from Brazil. Revista Brasileira de Parasitologia Veterinária, 28(1), 140-144. doi: 10.1590/S1984-296120180068 DOI: https://doi.org/10.1590/s1984-296120180068
Inokuma, H., Raoult, D., & Brouqui, P. (2000). Detection of Ehrlichia platys DNA in brown dog ticks (Rhipicephalus sanguineus) in Okinawa Island, Japan. Journal of Clinical Microbiology, 38(11), 4219-4221. doi: 10.1128/JCM.38.11.4219-4221.2000 DOI: https://doi.org/10.1128/JCM.38.11.4219-4221.2000
Levin, M. L., Nicholson, W. L., Massung, R. F., Sumner, J. W., & Fish, D. (2002). Comparison of the reservoir competence of medium-sized mammals and Peromyscus leucopus for Anaplasma phagocytophilum in Connecticut. Vector Borne and Zoonotic Diseases, 2(3), 125-136. doi: 10.1089/15303660260613693 DOI: https://doi.org/10.1089/15303660260613693
Lledó, L., Giménez-Pardo, C., Domínguez-Peñafiel, G., Sousa, R., Gegúndez, M. I., Casado, N., & Criado, A. (2010). Molecular detection of hemoprotozoa and Rickettsia species in arthropods collected from wild animals in the Burgos Province, Spain. Vector-Borne and Zoonotic Diseases, 10(8), 735-738. doi: 10.1089/vbz.2009.0114 DOI: https://doi.org/10.1089/vbz.2009.0114
Lopes, M. G., Muñoz-Leal, S., Lima, J. T. R. de, Fournier, G. F. D. S. R., Igor da Cunha, L. A., Martins, T. F., Ramirez, D. G., Gennari, S. M., & Labruna, M. B. (2018). Ticks, rickettsial and erlichial infection in small mammals from Atlantic forest remnants in northeastern Brazil. International Journal for Parasitology: Parasites and Wildlife, 7(3), 380-385. doi: 10.1016/j.ijppaw.2018.10.001 DOI: https://doi.org/10.1016/j.ijppaw.2018.10.001
Melo, A. L. T., Aguiar, D. M. D., Spolidorio, M. G., Yoshinari, N. H., Matushima, E. R., Labruna, M. B., & Horta, M. C. (2016). Serological evidence of exposure to tick-borne agents in opossums (Didelphis spp.) in the state of São Paulo, Brazil. Revista Brasileira de Parasitologia Veterinária, 25(3), 348-352. doi: 10.1590/S1984-29612016028 DOI: https://doi.org/10.1590/S1984-29612016028
Oliveira, G. M. B. de, Silva, I. W. G. da, Evaristo, A. M. D. C. F., Azevedo Serpa, M. C. de, Campos, A. N. S., Dutra, V., Nakazato, L., Aguiar, D. M., Labruna, M. B., & Horta, M. C. (2020). Tick-borne pathogens in dogs, wild small mammals and their ectoparasites in the semi-arid Caatinga biome, northeastern Brazil. Ticks and Tick-borne Diseases, 11(4), 101-409. doi: 10.1016/j.ttbdis.2020.101409 DOI: https://doi.org/10.1016/j.ttbdis.2020.101409
Onofrio, V. C., Barros-Battesti, D. M., Labruna, M. B., & Faccini, J. L. H. (2009). Diagnoses of and illustrated key to the species of Ixodes Latreille, 1795 (Acari: Ixodidae) from Brazil. Systematic Parasitology, 72(2), 143-157. doi: 10.1007/s11230-008-9169-z DOI: https://doi.org/10.1007/s11230-008-9169-z
Onofrio, V. C., Guglielmone, A. A., Barros-Battesti, D. M., Gianizella, S. L., Marcili, A., Quadros, R. M., Marques, S., & Labruna, M. B. (2020). Description of a new species of Ixodes (Acari: Ixodidae) and first report of Ixodes lasallei and Ixodes bocatorensis in Brazil. Ticks and Tick-Borne Diseases, 11(4), 101-423. doi: 10.1016/j.ttbdis.2020.101423 DOI: https://doi.org/10.1016/j.ttbdis.2020.101423
Ribeiro, C. M., Matos, A. C., Azzolini, T., Bones, E. R., Wasnieski, E. A., Richini-Pereira, V. B., Lucheis, S. B., & Vidotto, O. (2017). Molecular epidemiology of Anaplasma platys, Ehrlichia canis and Babesia vogeli in stray dogs in Paraná, Brazil. Pesquisa Veterinária Brasileira, 37(2), 129-136. doi: 10.1590/S0100-736X2017000200006 DOI: https://doi.org/10.1590/s0100-736x2017000200006
Santoro, M., Veneziano, V., D’Alessio, N., Di Prisco, F., Lucibelli, M. G., Borriello, G., Cerrone, A., Dantas-Torres, F., Latrofa, M. S., Otranto, D., & Galiero, G. (2016). Molecular survey of Ehrlichia canis and Coxiella burnetii infections in wild mammals of southern Italy. Parasitology Research, 115(11), 4427-4431. doi: 10.1007/s00436-016-5213-0 DOI: https://doi.org/10.1007/s00436-016-5213-0
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