Effects of insulin on sperm cell quality in ram semen cooled at 5°C
DOI:
https://doi.org/10.5433/1679-0359.2021v42n6SUPL2p3803Keywords:
Cooling, Glucose, Semen extender, Sheep, Spermatozoa.Abstract
Insulin is present in the seminal plasma and is involved in sperm activities like motility and capacitation. However, the effects of insulin on the viability of cooled ram sperm are not fully understood. Therefore, the objective of the current study was to evaluate the effect of insulin addition on ram sperm maintained at 5ºC. Sperm samples were collected from six healthy, mature Santa Inês rams. The ejaculates were divided into two aliquots with (insulin group) or without (control group) insulin (3 IU mL-1) in the semen extender, and then cooled at 5°C for 48 hours. Subsequently, the sperm cells were evaluated for motility and kinetics using computer-assisted semen analysis. The samples were evaluated for acrosomal integrity by fluorescein using isothiocyanate combined with peanut agglutinin (FITC-PNA) and membrane functionality by the hypoosmotic swelling test. The semen analysis was performed after 24 or 48 hours of cooling. There was an increased percentage of progressive sperm motility (%), straightness (%), linearity (%) and beat caudal frequency (Hz) in the insulin group after 24 and 48 hours of cooling (p < 0.05). However, insulin did not affect total sperm motility, sperm velocities (VSL, VAP and VCL) (µm seg-1), acrosomal integrity and membrane functionality during cooling (p > 0.05). In conclusion, the addition of 3 IU mL-1 insulin to ram semen extender improved the quality of sperm motility after cooling.Downloads
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References
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Leisegang, K., Bouic, P. J., Menkveld, R., & Henkel, R. R. (2014). Obesity is associated with increased seminal insulin and leptin alongside reduced fertility parameters in a controlled male cohort. Reproductive Biology and Endocrinology, 12(34), 34. doi: 10.1186/1477-7827-12-34
Makarevich, A., Spalekova, E., Olexikova, L., Kubovicova, E., & Hegedusova, Z. (2014). Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa. Zygote, 22(3), 305-313. doi: 10.1017/S0967199412000500
Meneses, M. J., Borges, D. O., Dias, T. R., Martins, F. O., Oliveira, P. F., Macedo, M. P., & Alves, M. G. (2019). Knockout of insulin-degrading enzyme leads to mice testicular morphological changes and impaired sperm quality. Molecular and Cellular Endocrinology, 486(1), 11-17. doi: 10.1016/j. mce.20 19.02.011
Morris, G. J., Acton, E., Murray, B. J., & Fonseca, F. (2012). Freezing injury: the special case of the sperm cell. Cryobiology, 64(2), 71-80. doi: 10.1016/j.cryobiol.2011.12.002
Palacín, I., Yániz, J. L., Fantova, E., Blasco, M. E., Quintín-Casorrán, F. J., Sevilla-Mur, E., & Santolaria, P. (2012). Factors affecting fertility after cervical insemination with cooled semen in meat sheep. Animal Reproduction Science, 132(3), 139-144. doi: 10.1016/j.anireprosci.2012.05.005
Pergialiotis, V., Prodromidou, A., Frountzas, M., Korou, L. M., Vlachos, G. D., & Perrea, D. (2016). Diabetes mellitus and functional sperm characteristics: A meta-analysis of observational studies. Journal of Diabetes and Its Complications, 30(6), 1167-1176. doi: 10.1016/j.jdiacomp.2016.04.002
Selvaraju, S., Krishnan, B. B., Archana, S. S., & Ravindra, J. P. (2016). IGF1 stabilizes sperm membrane proteins to reduce cryoinjury and maintain post-thaw sperm motility in buffalo (Bubalus bubalis) spermatozoa. Cryobiology, 73(1), 55-62. doi: 10.1016/j.cryobiol.2016.05.012
Shokri, S., Ebrahimi, S. M., Ziaeipour, S., & Nejatbakhsh, R. (2019). Effect of insulin on functional parameters of human cryopreserved sperms. Cryobiology, 87(1), 68-73. doi: 10.1016/j.cryobiol.2019. 02.002
Silva, D. M., Zangeronimo, M. G., Murgas, L. D. S., Rocha, L. G. P., Chaves, B. R., Pereira, B. A., & Cunha, E. C. P. (2011) Addition of IGF-I to storage-cooled boar semen and its effect on sperm quality. Growth Hormone & IGF Research, 21(6), 325-330. doi: 10.1016/j.ghir.2011.08.002
Srivastava, N., Srivastava, S. K., Ghosh, S. K., Kumar, A., Perumal, P., & Jerome, A. (2013). Acrosome membrane integrity and cryocapacitation are related to cholesterol content of bull spermatozoa. Asian Pacific Journal of Reproduction, 2(2), 126-131. doi: 10.1016/S2305-0500(13)60132-3
van Tilburg, M. F., Silva, J. F. S., Dias, A. J. B., Quirino, C. R., & Fagundes, B. (2008). Influência da insulina na congelabilidade do sêmen de ovino. Ciência Animal Brasileira, 9(3), 731-739.
Wang, G., Guo, Y., Zhou, T., Shi, X., Yu, J., Yang, Y.,... Sha, J. (2013). In-depth proteomic analysis of the human sperm reveals complex protein compositions. Journal of Proteomics, 79(1), 114-122. doi: 10.10 16/j.jprot.2012.12.008
Weerakoon, W. W. P. N., Sakase, M., Kohama, N., & Kawate, N. (2020). Plasma estradiol-17β, cortisol, and insulin concentrations and serum biochemical parameters surrounding puberty in Japanese Black beef bulls with normal and abnormal semen. Theriogenology, 148(1), 18-26. doi: 10.1016/j.theriogenology. 2020.02.035
Yániz, J. L., Palacín, I., Vicente-Fiel, S., Sánchez-Nadal, J. A., & Santolaria, P. (2015). Sperm population structure in high and low field fertility rams. Animal Reproduction Science, 156(1), 128-134. doi: 10. 1016/j.anireprosci.2015.03.012
Zhu, Z., Umehara, T., Tsujita, N., Kawai, T., Goto, M., Cheng, B.,... Shimada, M. (2020). Itaconate regulates the glycolysis/pentose phosphate pathway transition to maintain boar sperm linear motility by regulating redox homeostasis. Free Radical Biology and Medicine, 159(1), 44-53. doi: 10.1016/j.freeradbiomed. 2020.07.008
Aitken, R. J., Curry, B. J., Shokri, S., Pujianto, D. A., Gavriliouk, D., Gibb, Z. Baker, M. A. (2021). Evidence that extrapancreatic insulin production is involved in the mediation of sperm survival. Molecular and Cellular Endocrinology, 526(1), 111193. doi: 10.1016/j.mce.2021.111193
Baumgard, L. H., Hausman, G. J., & Sanz Fernandez, M. V. (2016). Insulin: pancreatic secretion and adipocyte regulation. Domestic Animal Endocrinology, 54(1), 76-84. doi: 10.1016/j.domaniend.2015. 07.001
Cappello, A. R., Guido, C., Santoro, A., Santoro, M., Capobianco, L., Montanaro, D., Aquila, S. (2012). The mitochondrial citrate carrier (CIC) is presented and regulates insulin secretion by human male gamete. Endocrinology, 153(1), 1743-1754. doi: 10.1210/en.2011-1562
Carpino, A., Rago, V., Guido, C., Casaburi, I., & Aquila, S. (2010) Insulin and IR-beta in pig spermatozoa: a role of the hormone in the acquisition of fertilizing ability. International Journal of Andrology, 33(1), 554-562. doi: 10.1111/j.1365-2605.2009.00971.x
Cunha, E. C. P., Zangeronimo, M. G., Murgas, L. D. S., Braga, D. E., Pereira, B. A., Rocha, L. G. P.,... Pereira, L. J. (2012). Insulin addition to swine semen diluted and cooled at 15 ºC. Revista Brasileira de Zootecnia, 41(1), 1060-1064. doi: 10.1590/S1516-35982012000400031
Curtis, M. P., Kirkman-Brown, J. C., Connolly, T. J., & Gaffney, E. A. (2012). Modelling a tethered mammalian sperm cell undergoing hyperactivation. Journal of Theoretical Biology, 309(1), 1-10. doi: 10.1016/j.jtbi.2012.05.035
Dias, T. R., Alves, M. G., Silva, B. M., & Oliveira, P. F. (2014). Sperm glucose transport and metabolism in diabetic individuals. Molecular and Cellular Endocrinology, 396(1), 37-45. doi: 10.1016/j.mce.2014. 08.005
El-Badry, D. A., El Sisy, G. A., & Abo El-Maaty, A. M. (2016). Seminal plasma hormonal profile of Arabian stallions that are classified ‘good’ or ‘poor’ for semen freezing. Asian Pacific Journal of Reproduction, 5(6), 453-458. doi: 10.1016/j.apjr.2016.10.012
Gürler, H., Malama, E., Heppelmann, M., Calisici, O., Leiding, C., Kastelic, J. P., & Bollwein, H. (2016). Effects of cryopreservation on sperm viability, synthesis of reactive oxygen species, and DNA damage of bovine sperm. Theriogenology, 86(2), 562-571. doi: 10.1016/j.theriogenology.2016.02.007
Hering, D. M., Olenski, K., & Kaminski, S. (2014). Genome-wide association study for poor sperm motility in Holstein-Friesian bulls. Animal Reproduction Science, 146(3-4), 89-97. doi: 10.1016/j. anireprosci. 2014.01.012
Kilkenny, C., Browne, W. J., Cuthill, I. C., Emerson, M., & Altman, D. G. (2012). Improving bioscience research reporting: The ARRIVE guidelines for reporting animal research. Osteoarthritis and Cartilage, 20(4), 256-260. doi: 10.1016/j.joca.2012.02.010
Laskowski, D., Sjunnesson, Y., Humblot, P., Andersson, G., Gustafsson, H., & Båge, R. (2016). The functional role of insulin in fertility and embryonic development - What can we learn from the bovine model? Theriogenology, 86(1), 457-464. doi: 10.1016/j.theriogenology.2016.04.062
Leisegang, K., Bouic, P. J., Menkveld, R., & Henkel, R. R. (2014). Obesity is associated with increased seminal insulin and leptin alongside reduced fertility parameters in a controlled male cohort. Reproductive Biology and Endocrinology, 12(34), 34. doi: 10.1186/1477-7827-12-34
Makarevich, A., Spalekova, E., Olexikova, L., Kubovicova, E., & Hegedusova, Z. (2014). Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa. Zygote, 22(3), 305-313. doi: 10.1017/S0967199412000500
Meneses, M. J., Borges, D. O., Dias, T. R., Martins, F. O., Oliveira, P. F., Macedo, M. P., & Alves, M. G. (2019). Knockout of insulin-degrading enzyme leads to mice testicular morphological changes and impaired sperm quality. Molecular and Cellular Endocrinology, 486(1), 11-17. doi: 10.1016/j. mce.20 19.02.011
Morris, G. J., Acton, E., Murray, B. J., & Fonseca, F. (2012). Freezing injury: the special case of the sperm cell. Cryobiology, 64(2), 71-80. doi: 10.1016/j.cryobiol.2011.12.002
Palacín, I., Yániz, J. L., Fantova, E., Blasco, M. E., Quintín-Casorrán, F. J., Sevilla-Mur, E., & Santolaria, P. (2012). Factors affecting fertility after cervical insemination with cooled semen in meat sheep. Animal Reproduction Science, 132(3), 139-144. doi: 10.1016/j.anireprosci.2012.05.005
Pergialiotis, V., Prodromidou, A., Frountzas, M., Korou, L. M., Vlachos, G. D., & Perrea, D. (2016). Diabetes mellitus and functional sperm characteristics: A meta-analysis of observational studies. Journal of Diabetes and Its Complications, 30(6), 1167-1176. doi: 10.1016/j.jdiacomp.2016.04.002
Selvaraju, S., Krishnan, B. B., Archana, S. S., & Ravindra, J. P. (2016). IGF1 stabilizes sperm membrane proteins to reduce cryoinjury and maintain post-thaw sperm motility in buffalo (Bubalus bubalis) spermatozoa. Cryobiology, 73(1), 55-62. doi: 10.1016/j.cryobiol.2016.05.012
Shokri, S., Ebrahimi, S. M., Ziaeipour, S., & Nejatbakhsh, R. (2019). Effect of insulin on functional parameters of human cryopreserved sperms. Cryobiology, 87(1), 68-73. doi: 10.1016/j.cryobiol.2019. 02.002
Silva, D. M., Zangeronimo, M. G., Murgas, L. D. S., Rocha, L. G. P., Chaves, B. R., Pereira, B. A., & Cunha, E. C. P. (2011) Addition of IGF-I to storage-cooled boar semen and its effect on sperm quality. Growth Hormone & IGF Research, 21(6), 325-330. doi: 10.1016/j.ghir.2011.08.002
Srivastava, N., Srivastava, S. K., Ghosh, S. K., Kumar, A., Perumal, P., & Jerome, A. (2013). Acrosome membrane integrity and cryocapacitation are related to cholesterol content of bull spermatozoa. Asian Pacific Journal of Reproduction, 2(2), 126-131. doi: 10.1016/S2305-0500(13)60132-3
van Tilburg, M. F., Silva, J. F. S., Dias, A. J. B., Quirino, C. R., & Fagundes, B. (2008). Influência da insulina na congelabilidade do sêmen de ovino. Ciência Animal Brasileira, 9(3), 731-739.
Wang, G., Guo, Y., Zhou, T., Shi, X., Yu, J., Yang, Y.,... Sha, J. (2013). In-depth proteomic analysis of the human sperm reveals complex protein compositions. Journal of Proteomics, 79(1), 114-122. doi: 10.10 16/j.jprot.2012.12.008
Weerakoon, W. W. P. N., Sakase, M., Kohama, N., & Kawate, N. (2020). Plasma estradiol-17β, cortisol, and insulin concentrations and serum biochemical parameters surrounding puberty in Japanese Black beef bulls with normal and abnormal semen. Theriogenology, 148(1), 18-26. doi: 10.1016/j.theriogenology. 2020.02.035
Yániz, J. L., Palacín, I., Vicente-Fiel, S., Sánchez-Nadal, J. A., & Santolaria, P. (2015). Sperm population structure in high and low field fertility rams. Animal Reproduction Science, 156(1), 128-134. doi: 10. 1016/j.anireprosci.2015.03.012
Zhu, Z., Umehara, T., Tsujita, N., Kawai, T., Goto, M., Cheng, B.,... Shimada, M. (2020). Itaconate regulates the glycolysis/pentose phosphate pathway transition to maintain boar sperm linear motility by regulating redox homeostasis. Free Radical Biology and Medicine, 159(1), 44-53. doi: 10.1016/j.freeradbiomed. 2020.07.008
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Published
2021-10-08
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van Tilburg, M. F., Oliveira, R. V. de, Gadelha, C. R. F., Fagundes, B., Dias, A. J. B., Quirino, C. R., & Silva, J. F. S. (2021). Effects of insulin on sperm cell quality in ram semen cooled at 5°C. Semina: Ciências Agrárias, 42(6SUPL2), 3803–3812. https://doi.org/10.5433/1679-0359.2021v42n6SUPL2p3803
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