Inactivation kinetics of peroxidase from coconut water

Authors

  • Rafael da Costa Ilhéu Fontan Universidade Estadual do Sudoeste da Bahia
  • Lizzy Ayra Pereira Alcântara Universidade Federal de Viçosa
  • Saint Clair Assis Laje Neto Universidade Estadual do Sudoeste da Bahia
  • Renata Cristina Ferreira Bonomo Universidade Estadual do Sudoeste da Bahia
  • Gabrielle Cardoso Reis Fontan Universidade Estadual do Sudoeste da Bahia

DOI:

https://doi.org/10.5433/1679-0359.2012v33n1p249

Keywords:

D value, Z value, Activation energy, Q10, Enzyme activity.

Abstract

The aim of this project was study the thermal inactivation kinetics of the peroxidase enzyme (POD) present in the green coconut water, being determined the parameters D values, z value and Q10, besides the Arrhenius’ activation energy (Ea) to the process. Nine time/temperature binomials for the thermal treatment at three temperatures (75°C, 85°C and 95°C) and maintenance times from 1min to 30 min, besides a control sample without thermal treatment. D values were obtained by linear regression, being equals to 833,3 min, 35,6min and 1,63min at 75°C, 85°C and 95°C, respectively, while the z value was equal to 7,4°C. These values demonstrate that POD of coconut water is thermal resistant to inactivation, being such processes strongly affected by temperature. Such fact is also verified by the high values of Ea (332,3 kJ/mol) and Q10 (22,7), which indicate that a high amount of energy is required for the POD inactivation and an increase of temperature elevate the inactivation rate.

Author Biographies

Rafael da Costa Ilhéu Fontan, Universidade Estadual do Sudoeste da Bahia

Prof. Assistente, Dept° de Tecnologia Rural e Animal, DTRA, Universidade Estadual do Sudoeste da Bahia, UESB, Itapetinga, BA.

Lizzy Ayra Pereira Alcântara, Universidade Federal de Viçosa

Pós-Graduanda em Ciência e Tecnologia de Alimentos, Dept° de Tecnologia de Alimentos, Universidade Federal de Viçosa, UFV, Viçosa, MG.

Saint Clair Assis Laje Neto, Universidade Estadual do Sudoeste da Bahia

Engenheiro de Alimentos, DTRA, UESB, Itapetinga, BA.

Renata Cristina Ferreira Bonomo, Universidade Estadual do Sudoeste da Bahia

Pesquisadora Nível 2 do CNPq, Profª Titular, DTRA, UESB, Itapetinga, BA.

 

Gabrielle Cardoso Reis Fontan, Universidade Estadual do Sudoeste da Bahia

Profª Assistente, DTRA, UESB, Itapetinga, BA.

Published

2012-04-05

How to Cite

Fontan, R. da C. I., Alcântara, L. A. P., Laje Neto, S. C. A., Bonomo, R. C. F., & Fontan, G. C. R. (2012). Inactivation kinetics of peroxidase from coconut water. Semina: Ciências Agrárias, 33(1), 249–258. https://doi.org/10.5433/1679-0359.2012v33n1p249

Issue

Section

Articles