Chromosome doubling by colchicine injection and haploidy induction in tropical genotypes of common and supersweet corn

Authors

DOI:

https://doi.org/10.5433/1679-0359.2025v46n2p517

Keywords:

Zea mays var. saccharate, Haploidy induction rate, Double-haploids, Gimnogenetic inducer

Abstract

The double-haploid technology in maize was developed in temperate environments, using germplasm and inducers adapted for these environments, with the aim of accelerating the obtaining of homozygous inbred lines. Therefore, to advance this technology in tropical environments, research involving germplasm and inducers adapted for this environment is necessary. The objectives were to determine the haploidy induction rate in tropical common and supersweet corn populations, employing a tropicalized gymnogenetic inducer population, and to identify the effectiveness of chromosome doubling by colchicine injection. The haploidy inducer PI4003 was used as a male parent and crossed with 25 tropical maize populations (23 common and two supersweet). The putative haploid seeds, classified by the R1-nj gene marker, were sown in trays with peat during the 2020/21 and 2021/22 harvest seasons, and 100 µL of a solution of 0.125% colchicine and 0.5% dimethyl sulfoxide was injected into each haploid seedling at the V2 stage. The next day, the treated seedlings were transplanted to an irrigated field. The PI4003 inducer presented a haploidy induction rate ranging from 0.9% to 5%. There were significant differences to tropical genotypes and harvests for survival rates, reproduction rates, and overall success rates. The overall success rate ranged from 2.9% to 34%. The chromosome doubling method by colchicine injection proved to be effective, with lower colchicine consumption per haploid seedling treated and a reduction in the generation of toxic waste for disposal.

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Author Biographies

Leticia de Freitas de Souza, Universidade Estadual de Londrina

M.e., Universidade Estadual de Londrina, UEL, Londrina, PR, Brazil.

Juliana Moraes Machado de Oliveira, Universidade Estadual de Londrina

Student, Graduate Program in Agronomy, Universidade Estadual de Londrina, UEL, Londrina, PR, Brazil.

Vitor Joaquim de Lucena, Universidade Estadual de Londrina

Student, Graduate Program in Agronomy, Universidade Estadual de Londrina, UEL, Londrina, PR, Brazil.

Otavio Gabriel Lalau Hoda, Universidade Estadual de Londrina

Agronomist, Universidade Estadual de Londrina, UEL, Londrina, PR, Brazil.

Iran de Azevedo Duarte, Universidade Estadual de Londrina

Dr., Universidade Estadual de Londrina, UEL, Londrina, PR, Brazil.

Bruno Figueiró Fregonezi, Fundação de Estudos Agrários Luiz de Queiroz

Student, Graduate Program in Genetics and Plant Breeding, Escola Superior de Agricultura "Luiz de Queiroz", USP, ESALQ, Piracicaba, SP, Brazil.

Josué Maldonado Ferreira, Universidade Estadual de Londrina

Prof. Dr., Department of General Biology, Universidade Estadual de Londrina, UEL, Londrina, PR, Brazil.

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Published

2025-03-17

How to Cite

Souza, L. de F. de, Oliveira, J. M. M. de, Lucena, V. J. de, Hoda, O. G. L., Duarte, I. de A., Fregonezi, B. F., & Ferreira, J. M. (2025). Chromosome doubling by colchicine injection and haploidy induction in tropical genotypes of common and supersweet corn. Semina: Ciências Agrárias, 46(2), 517–532. https://doi.org/10.5433/1679-0359.2025v46n2p517

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