Evaluation histological, immunofluorescence and immunohistochemistry techniques for the postmortem diagnostic of the swine toxoplasmosis.

Authors

  • Neusa Maria Alves Viotti Universidade Estadual de Londrina
  • Roberta Lemos Freire Universidade Estadual de Londrina
  • Italmar Teodorico Navarro Universidade Estadual de Londrina
  • Odilon Vidotto Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/1679-0359.1995v16n1p107

Keywords:

Toxoplasmosis, Histopatology, Immunohistochemistry, Immunofluorescence, Swine

Abstract

Six 120 days old Landrace/Large-white swines with were inoculated with taquizoites of the AS 28, LIV-IV and N Toxoplasma gondii strains and analysed under clinical, sorological, parasitological and histopathological aspects. In order to evaluate some postmortem diagnostic techniques, three swines were sacrificed in the acute phase and three swines in the chronic phase of the infection. Formalin-fixed and parafin-embedded tissues were submitted to Eosin-Hematoxilin, Indirect Immunofluorescence (IFA) and Peroxidase Anti-Peroxidase (PAP) techniques. The Eosin-Hematoxilin showed to be efficient in the analysis of the morphological alterations in the tissues, but did not detect any form of the parasite. Both, IFA and PAP revealed taquizoites of T. gondii, although the last one showed to be more effective because it also allowed an analysis of the morphological tissue alterations.

 

Author Biographies

Neusa Maria Alves Viotti, Universidade Estadual de Londrina

Depto. de Medicina Veterinária / CCA / Universidade Estadual de Londrina, Caixa Postal 6001, Londrina, PR, Brasil, CEP 86051-970.

 

Roberta Lemos Freire, Universidade Estadual de Londrina

Depto. de Medicina Veterinária / CCA / Universidade Estadual de Londrina, Caixa Postal 6001, Londrina, PR, Brasil, CEP 86051-970.

Italmar Teodorico Navarro, Universidade Estadual de Londrina

Depto. de Medicina Veterinária / CCA / Universidade Estadual de Londrina, Caixa Postal 6001, Londrina, PR, Brasil, CEP 86051-970.

Odilon Vidotto, Universidade Estadual de Londrina

Depto. de Medicina Veterinária / CCA / Universidade Estadual de Londrina, Caixa Postal 6001, Londrina, PR, Brasil, CEP 86051-970.

Published

1995-01-25

How to Cite

Viotti, N. M. A., Freire, R. L., Navarro, I. T., & Vidotto, O. (1995). Evaluation histological, immunofluorescence and immunohistochemistry techniques for the postmortem diagnostic of the swine toxoplasmosis. Semina: Ciências Agrárias, 16(1), 107–114. https://doi.org/10.5433/1679-0359.1995v16n1p107

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