Occurrence of Lawsonia intracellularis in horses raised in three regions of the state of Paraná, Brazil

Authors

DOI:

https://doi.org/10.5433/1679-0359.2021v42n5p2867

Keywords:

Equine proliferative enteropathy, Immunoperoxidase monolayer, Polymerase chain reaction.

Abstract

Lawsonia intracellularis is a bacterium already described in several species and most prevalent in pigs, in which it causes enteric problems. Horses can also be affected, developing a disease known as equine proliferative enteropathy, which results from the proliferation of intestinal crypt cells in response to infection by the bacterium. Despite the existence of reports of the disease in several countries, including Brazil, there are still no reports of the disease or epidemiological studies of its occurrence in symptomatic or asymptomatic horses in the state of Paraná. Thus, the present study was conducted to examine the occurrence of L. intracellularis in asymptomatic horses raised in the west, northwest and north regions of Paraná by means of serological testing and the real-time polymerase chain reaction (qPCR) technique. In the serological approach, the immunoperoxidase monolayer assay (IPMA) technique was employed. Feces were processed and subjected to qPCR. In total, samples were collected from 162 animals from 20 farms. Of these, 9/162 (5.55%) showed specific antibodies against L. intracellularis. Real-time PCR, on the other hand, identified 7/162 (4.32%) fecal samples positive for the presence of the bacterium. When the techniques were compared, none of the samples was positive by both, demonstrating that, for a better diagnosis, they must be performed together. In contrast to most reports in horses, the present study describes higher serological and molecular occurrence in animals older than two years. These results are of great epidemiological relevance, as they indicate that the bacterium is present in the sampled regions of the state of Paraná. Therefore, the disease must be included in the differential diagnosis of diseases with similar clinical manifestations.

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Author Biographies

Tatiane Caleffo, União de Ensino do Sudoeste do Paraná

Prof. Msc., Sindicato da Educação do Sudoeste do Paraná, União de Ensino do Sudoeste do Paraná, UNISEP, Dois Vizinhos, PR, Brazil.

Vinicius Dahm, Universidade Federal do Paraná

Student of the Undergraduate Course in Veterinary Medicine, Universidade Federal do Paraná, UFPR, Palotina, PR, Brazil.

Jéssica Gonçalves dos Santos, Iowa State University

PhD Candidate of the Veterinary Microbiology and Preventive Medicine Program, Iowa State University, ISU, Ames, IA, United States of America.

Arthur Colombari Cheng, Universidade Federal do Paraná

Anatomic Pathology Resident, Veterinary Medicine Residency Program, Universidade Federal do Paraná, UFPR, Palotina, PR, Brazil.

Mayane Faccin, Texas A&M University

Graduate Assistant, Veterinary Pathobiology, Texas A&M University, TAMU, College Station, TX, United States of America.

Monica Regina de Matos, Pontifícia Universidade Católica do Paraná

Profa Msc., Pontifícia Universidade Católica do Paraná, PUCPR, Toledo, PR, Brazil.

João Pedro Cavasin, College of Veterinary Medicine, Cornell University

Anatomic Pathology Resident, Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, CU, Ithaca, NY, United States of America.

Michelle de Paula Gabardo, Instituto Federal de Minas Gerais

Profa Dra, Department of Agricultural Sciences, Instituto Federal de Minas Gerais, IFMG, Bambuí, MG, Brazil.

Roberto Mauricio Carvalho Guedes, Universidade Federal de Minas Gerais

Prof. Dr., Department of Veterinary Clinic and Surgery, Universidade Federal de Minas Gerais, UFMG, Belo Horizonte, MG, Brazil.

Aline de Marco Viott, Universidade Federal do Paraná

Profa Dra, Department of Veterinary Sciences, UFPR, Palotina, PR, Brazil.

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Published

2021-07-02

How to Cite

Caleffo, T., Dahm, V., Santos, J. G. dos, Cheng, A. C., Faccin, M., Matos, M. R. de, … Viott, A. de M. (2021). Occurrence of Lawsonia intracellularis in horses raised in three regions of the state of Paraná, Brazil. Semina: Ciências Agrárias, 42(5), 2867–2876. https://doi.org/10.5433/1679-0359.2021v42n5p2867

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