Application of hormonal subdoses at the Bai Hui acupoint for estrus synchronization in sheep

Authors

DOI:

https://doi.org/10.5433/1679-0359.2021v42n4p2359

Keywords:

Acupuncture, Equine chorionic gonadotropin, Prostaglandin F2alfa.

Abstract

Here, the efficiency of applying subdoses of equine chorionic gonadotropin (eCG) and prostaglandin F2 alfa (PGF2alfa) at the Bai Hui (BH) acupoint for estrus synchronization in sheep was evaluated. Thirty Santa Inês ewes received intravaginal sponges containing 60 mg medroxyprogesterone acetate for 7 days. The animals were divided into five treatments (T) (n=6): T1 (control), 132.5 ug PGF2 alfa (100% of dose) and 300 IU eCG (100% of dose), both intramuscularly (IM); T2, 39.75 ug PGF2 alfa (30% of dose) at the BH acupoint and 300 IU eCG (100% of dose) IM; T3, 132.5 ug PGF2 alfa (100% of dose) IM and 90 IU eCG (30% of dose) at the BH acupoint; T4, 39.75 ug PGF2 alfa (30% of dose) and 90 IU eCG (30% of dose), both at the BH acupoint; and T5, 39.75 ug PGF2 alfa (30% of dose) and 90 IU eCG (30% of dose) both at a false acupoint (IM, at the same location as in T1). After 12 h of sponge removal and hormone application, the ewes were subjected to monitoring for estrus and mating and ultrasound assessments of follicular growth and ovulation time every 12 h. The data were evaluated for normality and subjected to analysis of variance, considering a 5% probability. There were no differences in the percentage of animals in estrus (93.3±9.1%), interval from sponge removal to estrus onset (48.1±6.0 h), interval from sponge removal to estrus end (69.6±5.4 h), duration of estrus (24.0±4.7 h), interval from sponge removal to ovulation (73.7±5.9 h), interval from estrus onset to ovulation (26.5±2.3 h), size of the largest follicle (6.7±0.4 mm), follicular growth rate (0.8±0.1 mm•day-1), number of ovulations (1.3±0.1), and plasma progesterone levels 7 days after ovulation (9.6±2.0 ng•mL-1). The cost of the synchronization protocol per animal was US$ 4.37, 4.12, 2.96, 2.71, and 2.71 for T1, T2, T3, T4, and T5, respectively, with 37.99% cost reduction using PGF2 alfa and eCG at 30% of the conventional doses. Therefore, the application of reduced PGF2 alfa (39.75 ug) and eCG (90 IU) doses at the BH or false acupoint effectively synchronized estrus and reduced protocol cost in sheep, indicating that the conventional doses are overestimated.

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Author Biographies

Diego Oliveira Souza, Universidade Federal do Recôncavo da Bahia

Master in Animal Science, Post-Graduate Program in Animal Science, Universidade Federal do Recôncavo da Bahia, UFRB, Cruz das Almas, BA, Brazil.

Carmo Emanuel Almeida Biscarde, Universidade Federal do Recôncavo da Bahia

Doctor in Animal Science in the Tropics, Post-Graduate Program in Animal Science in the Tropics, Universidade Federal da Bahia, UFBA, Salvador, BA, Brazil.

William Morais Machado, Universidade Estadual de Santa Cruz

Student of the Doctoral Course of the Post-Graduate Program in Animal Science, Universidade Estadual de Santa Cruz, UESC, Ilhéus, BA, Brazil.

Renan Luiz Albuquerque Vieira, Universidade Federal do Recôncavo da Bahia

Student of the Doctoral Course of the Post-Graduate Program in Animal Science in the Tropics, UFBA, Salvador, BA, Brazil.

Claudinéia Silva Mendes, Universidade Federal do Recôncavo da Bahia

Master in Animal Science, Post-Graduate Program in Animal Science, Universidade Federal do Recôncavo da Bahia, UFRB, Cruz das Almas, BA, Brazil.

Monna Lopes de Araújo, Universidade Federal da Bahia

Student of the Doctoral Course of the Post-Graduate Program in Animal Science in the Tropics, UFBA, Salvador, BA, Brazil.

Ana Lúcia Almeida Santana, Universidade Federal do Recôncavo da Bahia

Profª Drª, UFRB, Cruz das Almas, BA, Brazil.

Larissa Pires Barbosa, Universidade Federal do Recôncavo da Bahia

Profª Drª, UFRB, Cruz das Almas, BA, Brazil.

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Published

2021-05-20

How to Cite

Souza, D. O., Biscarde, C. E. A., Machado, W. M., Vieira, R. L. A., Mendes, C. S., Araújo, M. L. de, Santana, A. L. A., & Barbosa, L. P. (2021). Application of hormonal subdoses at the Bai Hui acupoint for estrus synchronization in sheep. Semina: Ciências Agrárias, 42(4), 2359–2370. https://doi.org/10.5433/1679-0359.2021v42n4p2359

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