Methodology of the tetrazolium test for identifying viable seeds of orchids of the genus Cattleya native to the Brazilian Cerrado
DOI:
https://doi.org/10.5433/1679-0359.2021v42n3p1351Keywords:
Seed bank, Native species, Orchidaceae, Viability test.Abstract
Orchids need measures for conservation of their species, among them the creation of seed banks. This study analyzes suitable methodologies to identify the viability of Cattleya seeds through the tetrazolium test. Seeds of Cattleya nobilior Rchb. and Cattleya walkeriana Gardn. were submitted to three preconditioning: no soaking (control); sucrose (10%); or distilled water. After 24 hours, they were submitted to tetrazolium solution with three types of conditioning: oven (40 °C); water bath (40 °C); or room temperature. Subsequently, the percentage of viable seeds (VS) was calculated and in vitro sowing was carried out. Germination percentage (G) was determined after 45 days. A completely randomized design was used for each species studied, with treatments arranged in a 3x3 factorial scheme (three preconditioning and three conditioning), with eight replicates of one tube each. The experiment was performed in duplicate. The highest viability values found in the tetrazolium test were close to those of germination (C. nobilior: VS = 88% e G = 97%; C. walkeriana: VS = 88% e G = 95%) for Cattleya species native to the Cerrado. The conditioning at ambient temperature had a greater effect on seed visualization for both species studied, suggesting that the tetrazolium test should be performed at this conditioning temperature.Downloads
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References
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Hosomi, S. T., Souza, T. B., Custódio, C. C., & Machado, N. B., Neto. (2017). Refining the tetrazolium test for evaluation of Cattleya labiata and C. tigrina seeds viability. Australian Journal of Crop Science, 11(10), 1320-1326. doi: 10.21475/ajcs.17.11.10.pne606
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Soares, J. S., Rosa, Y. B. C. J., Tatara, M. B., Sorgato, J. C., & Lemes, C. S. R. (2014). Identificação da viabilidade de sementes de orquídeas pelo teste de tetrazólio. Semina: Ciências Agrárias, 35(5), 2275-2284. doi: 10.5433/1679-0359.2014v35n5p2275
Suzuki, A. B. P., Bertoncelli, D. J., Alves, G. A. C., & Faria, R. T. (2018). Criopreservação de sementes da orquídea brasileira em extinção Cattleya granulosa Lindl. Iheringia, Série Botânica, 73(2), 146-150. doi: 10.21826/2446-8231201873206
Zahara, M., Datta, A., Boonkorkaew, P., & Mishra, A. (2017). The effects of different media, sucrose concentrations and natural additives on plantlet growth of Phalaenopsis Hybrid ‘pink’. Brazilian Archives of Biology and Technology, 60(1), 1-15. doi: 10.1590/1678-4324-2017160149
Flora do Brasil (2020). Jardim Botânico do Rio de Janeiro. Rio de Janeiro, RJ: JBRJ. Recuperado de http:// floradobrasil.jbrj.gov.br/
Carneiro, L. L. (2014). Pré-melhoramento genético, floração in vitro e criopreservação de orquídeas nativas do Cerrado. Tese de doutorado, Universidade Federal de Goiás, Goiânia, GO, Brasil.
Fay, M. F. (2018). Orchid conservation: how can we meet the challenges in the twenty-first century? Botanical Studies, 59(16), 1-6. doi: 10.1186/s40529-018-0232-z
Hengling, M. M., Gianeti, T. M., Hosomi, S. T., Machado, N. B., Neto, & Custódio, C. C. (2020). Storage of Brazilian Cattleya seeds from diverse biomes: lipid composition and effects on germination. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 11(1), 1-16. doi: 10.1080/ 11263504.2020.1762781
Hosomi, S. T. (2017). Sementes de orquídeas: conservação e avaliação de viabilidade. Tese de doutorado, Universidade do Oeste Paulista, Presidente Prudente, SP, Brasil.
Hosomi, S. T., Santos, R. B., Custódio, C. C., & Machado, N. B., Neto. (2011). Preconditioning Cattleya seeds to improve the efficacy of the tetrazolium test for viability. Seed Science and Technology, 39(1), 178-189. doi: 10.15258/sst.2011.39.1.15
Hosomi, S. T., Souza, T. B., Custódio, C. C., & Machado, N. B., Neto. (2017). Refining the tetrazolium test for evaluation of Cattleya labiata and C. tigrina seeds viability. Australian Journal of Crop Science, 11(10), 1320-1326. doi: 10.21475/ajcs.17.11.10.pne606
Ministério da Agricultura, Pecuária e Abastecimento (2009). Regras para análise de sementes. Brasília, DF: MAPA. Recuperado de https://www.abrates.org.br/files/regras_analise_de_sementes.pdf
Murashige, T., & Skoog, F. A. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiology Plantarum, 15(1), 473-497. doi: 10.1111/j.1399-3054.1962.tb08052.x
Oliveira, L. M., Carvalho, M. L. M., & Davide, A. C. (2005). Teste de tetrazólio para avaliação de sementes de Peltophorum dubium (Sprengel) Taubert - leguminosae caesalpinioideae. Cerne, 11(2), 159-166.
Oliveira, R. S., Batista, J. A. N., Proença, C. E. B., & Bianchetti, L. (1996). Efeito do fogo na floração de espécies de Orchidaceae em Cerrado. In H. S. Miranda, B. F. S. Dias, & C. H. Saito (Eds.), Impacto de queimadas em área de Cerrado e restinga (pp. 61-67). Brasília, DF: Universidade de Brasília.
Seaton, P. T., Hosomi, S. T., Custódio, C. C., Marks, T. R., Machado, N. B., Neto, & Pritchard, H. W. (2018). Orchid seed and pollen: a toolkit for long-term storage, viability assessment and conservation. In Y. Lee, & E. C. Yeung (Eds.), Orchid propagation: from laboratories to greenhouses - methods and protocols (pp. 71-98). New York, NY: Humana press.
Soares, J. S., Rosa, Y. B. C. J., Tatara, M. B., Sorgato, J. C., & Lemes, C. S. R. (2014). Identificação da viabilidade de sementes de orquídeas pelo teste de tetrazólio. Semina: Ciências Agrárias, 35(5), 2275-2284. doi: 10.5433/1679-0359.2014v35n5p2275
Suzuki, A. B. P., Bertoncelli, D. J., Alves, G. A. C., & Faria, R. T. (2018). Criopreservação de sementes da orquídea brasileira em extinção Cattleya granulosa Lindl. Iheringia, Série Botânica, 73(2), 146-150. doi: 10.21826/2446-8231201873206
Zahara, M., Datta, A., Boonkorkaew, P., & Mishra, A. (2017). The effects of different media, sucrose concentrations and natural additives on plantlet growth of Phalaenopsis Hybrid ‘pink’. Brazilian Archives of Biology and Technology, 60(1), 1-15. doi: 10.1590/1678-4324-2017160149
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Published
2021-03-19
How to Cite
Ribeiro, L. M., Sorgato, J. C., Soares, J. S., & Ramos, J. C. M. (2021). Methodology of the tetrazolium test for identifying viable seeds of orchids of the genus Cattleya native to the Brazilian Cerrado. Semina: Ciências Agrárias, 42(3), 1351–1360. https://doi.org/10.5433/1679-0359.2021v42n3p1351
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