Influencing factors for preparation of platelet-rich plasma in horses

Authors

DOI:

https://doi.org/10.5433/1679-0359.2021v42n4p2327

Keywords:

Platelet concentration, Double centrifugation., Standardization.

Abstract

There is no consensus in the literature as to the best platelet-rich plasma (PRP) acquisition method or the ideal platelet concentration to stimulate tissue repair. Besides that, most studies do not provide a clear and replicable description of the methods used, which makes standardization and result comparison difficult. Thus, this study aimed to accurately describe the method and evaluate factors influencing PRP preparation in equines. In this regard, two protocols were used, P1 and P2, based on two centrifugation methods, which differed in blood volume, speed and time, and platelet pipetting and resuspension. In conclusion, factors such as centrifugation strength and time are essential to obtain PRP with adequate platelet count. However, other factors such as tube type and plasma pipetting and resuspension methods directly interfere with the replicability of the technique, and hence influencing PRP applicability.

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Author Biographies

Ana Carolina Barros da Rosa Pedroso, Universidade Federal de Goiás

Postgraduate Student at Animal Science Graduate Program, Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, UFG, Goiânia, GO, Brazil.

Andréia da Costa Peixoto, Universidade Federal de Goiás

Postgraduate Student at Animal Science Graduate Program, Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, UFG, Goiânia, GO, Brazil.

Evelyn de Oliveira, Universidade Federal de Goiás

Postgraduate Student at Animal Science Graduate Program, Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, UFG, Goiânia, GO, Brazil.

Helena Tavares Dutra, Universidade Federal de Goiás

Postgraduate Student at Animal Science Graduate Program, Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, UFG, Goiânia, GO, Brazil.

Roberta Carvalho Basile, Centro Universitário Central Paulista

Profa Dra, Centro Universitário Central Paulista, UNICEP, São Carlos, SP, Brazil.

Luciana Ramos Gaston Brandstetter, Universidade Federal de Goiás

Profa Dra, Veterinary Medicine Department, Escola de Veterinária e Zootecnia, UFG, Goiânia, GO, Brazil.

Veridiana Maria Brianezi Dignani de Moura, Universidade Federal de Goiás

Profa Dra, Animal Pathology Department, Escola de Veterinária e Zootecnia, UFG, Goiânia, GO, Brazil.

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Published

2021-05-20

How to Cite

Pedroso, A. C. B. da R., Peixoto, A. da C., Oliveira, E. de, Dutra, H. T., Basile, R. C., Brandstetter, L. R. G., & Moura, V. M. B. D. de. (2021). Influencing factors for preparation of platelet-rich plasma in horses. Semina: Ciências Agrárias, 42(4), 2327–2338. https://doi.org/10.5433/1679-0359.2021v42n4p2327

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