Canines vaccinated against visceral leishmaniasis develop a serological response to the Leishmania braziliensis antigen
DOI:
https://doi.org/10.5433/1679-0359.2018v39n2p573Keywords:
American cutaneous leishmaniasis, Leishmania vaccines, Diagnosis, ELISA, Immunoblotting.Abstract
American cutaneous leishmaniasis (ACL) is a zoonosis caused by Leishmania, a protozoan. Common antigens occur in the strains found in America, which allow antigenic cross-reactivity. Therefore, multivalent vaccines can be used for this pathogen. In this study, we investigated the efficacy of two different commercial vaccines for visceral leishmaniasis to induce an immune response to the soluble L. (Viannia) braziliensis antigens. In 2014, 70 seronegative dogs from the municipality of Iúna (Espírito Santo State, Brazil) were vaccinated and serologically evaluated by ELISA and immunoblotting by using the soluble antigen of L. braziliensis. Of the 121 dogs initially selected, only 70 received vaccination because 51 dogs tested positive by ELISA, yielding a positive frequency of 42.14% in the asymptomatic group. These 70 dogs were divided into two equal groups and administered three doses of each vaccine, according to the manufacturers’ instructions. We found that the sera of dogs immunized with three doses of both vaccines A and B had antibodies against the soluble antigens of L. (V.) braziliensis, as determined by ELISA and immunoblotting 120 days post vaccination. Antibodies produced in response to vaccines A and B were found in 22/35 and 18/35 serum samples, respectively, at T1 (120 days), while 7/35 and 4/35 serum samples tested positive at T2 (240 days). Furthermore, immunoblotting allowed us to differentiate between vaccinated and asymptomatic dogs.Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2018 Semina: Ciências Agrárias
![Creative Commons License](http://i.creativecommons.org/l/by-nc/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
O Copyright dos manuscritos publicados pertence ao periódico. Como são publicados em um periódico de acesso aberto, eles estão disponíveis gratuitamente, para uso privado ou para fins educacionais e não comerciais.
A revista tem o direito de fazer, no documento original, alterações referentes às normas lingüísticas, ortografia e gramática, com o objetivo de garantir as normas padrão do idioma e a credibilidade da revista. No entanto, respeitará o estilo de escrita dos autores.
Quando necessário, alterações conceituais, correções ou sugestões serão encaminhadas aos autores. Nesses casos, o manuscrito deve ser submetido a uma nova avaliação após revisão.
A responsabilidade pelas opiniões expressas nos manuscritos é inteiramente dos autores.