Concentração inibitória mínima de óleos essenciais contra Staphylococcus aureus isolados de cães com otite externa
DOI:
https://doi.org/10.5433/1679-0359.2021v42n6SUPL2p3837Palavras-chave:
Fitoterapia, Otopatias, Antibacterianos, Antimicrobianos naturais, CIM.Resumo
A otite canina externa é uma doença que afeta o meato acústico externo de até 20% dos cães em algum período de suas vidas, sem predileção por raça, idade ou sexo. Trata-se de uma doença multifatorial que tem Staphylococcus aureus como um dos principais agentes etiológicos. Antimicrobianos constituem a base para o tratamento desta enfermidade, entretanto, devido ao incremento da resistência antimicrobiana, os fármacos convencionais tem se tornado pouco eficazes, o que requer a busca por terapias alternativas. Neste contexto, os óleos essenciais (OE) apresentam grande potencial terapêutico devido a sua ampla ação antimicrobiana. Este trabalho visou detectar a concentração inibitória mínima (CIM), a partir da qual foram obtidos os índices de MIC50 e MIC90 para a gentamicina e para os OE de Eugenia caryophyllata, Thymus vulgaris, Cymbopogon winterianus, Cymbopogon citratus e Cinnamomum cassia contra 62 Staphylococcus aureus isolados do meato acústico externo de cães otopatas. Todos os OE mostraram ação antibacteriana contra os microrganismos estudados e foram detectadas as seguintes CIM50 e CIM90: Eugenia caryophyllata, 2,42 mg mL-1e 7,45 mg mL-1; Thymus vulgaris, 9,51 mg mL-1 e 22,94 mg mL-1; Cymbopogon winterianus, 26,78 mg mL-1 e 157,79 mg mL-1; Cinnamomum cassia e Cymbopogon citratus CM menores que 16,48 e 27,81 mg mL-1, respectivamente já que estes apresentaram CIM única para todos os isolados testados. Para a gentamicina, foram obtidas CIM50 e CIM90 1ug mL-1 e 8 ug/m, respectivamente. Ademais, a faixa de CIM encontrada para o antibiótico variou de 0,5 a 128 ug mL-1, e os isolados foram classificados em susceptíveis [48 isolados (77,41%) - CIM na faixa de 0,5-4 ug mL-1], intermediários [8 isolados (12,90%) - (CIM = 8ug mL-1] e resistentes [6 isolados (9,68%) - CIM maior, menor 16]. Os resultados encontrados mostraram que os isolados de micro-organismos testados tem potencial para desenvolver resistência antimicrobiana a terapêutica tradicional e que os OE, de acordo com os resultados in vitro, apresentam potencial de uso terapêutico, entretanto futuros estudos devem ser realizados para avaliar a eficácia dos OE in vivo.Downloads
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Costa, G. M., Prado, I. A., Carvalho-Castro, G. dos A. de, Mian, G. F., Leite, C. A. L., Custódio, D. A. C., & Piccoli, R. H. (2021). Concentração inibitória mínima de óleos essenciais contra Staphylococcus aureus isolados de cães com otite externa. Semina: Ciências Agrárias, 42(6SUPL2), 3837–3854. https://doi.org/10.5433/1679-0359.2021v42n6SUPL2p3837
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