Simultaneous HPLC/UV Detection of Sulfonamides in Milk Using Fabric Phase Sorptive Extraction
DOI:
https://doi.org/10.5433/1679-0375.2024.v45.49727Keywords:
Sulfonamides, Milk, FPSE, HPLC/UVAbstract
The fabric phase sorptive extraction technique was employed for the simultaneous removal of sulfamethazine and sulfamethoxazole in milk. To achieve this, a cotton fabric was coated with a sol-gel solution of polyethylene glycol to serve as a substrate in the adsorption process. The coated and uncoated fabrics were characterized using x-ray energy dispersive spectroscopy and scanning electron microscopy, confirming the effectiveness of the coating procedure. Quantification of the analytes was conducted using high-performance liquid chromatography with ultraviolet detection, with a wavelength of 267 nm. The mobile phase that exhibited the best analytical separation and selectivity for the studied analytes in the presence of the complex milk matrix consisted of acetonitrile and water (30:70 v/v). The results of the evaluation of the removal capacity of sulfonamides were 35.30 % ± 1.09 for sulfamethazine and 42.79 % ± 1.68 for sulfamethoxazole, demonstrating the effectiveness of the adsorbent system and the methodology used in the extraction and detection of these compounds in milk.
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