Single cell protein production by Wickerhamomyces anomalus CCC32 using crude glycerol
DOI:
https://doi.org/10.5433/1679-0375.2019v40n2p179Keywords:
Agro industrial wastes, Fungi, FermentationAbstract
The objective of this work was to evaluate the production of single cell protein (SCP) by five yeasts using crude glycerol as carbon source, to select the most promising strain and optimize biomass and crude protein roduction by central composite design (CCD). Wickerhamomyces anomalus CCC32 was the most promising strain for SCP production with biomass production of 20 g L?1 and crude protein concentration of 33% after 84 h using crude glycerol 25 g L?1 , NaNO3 3.5 g L?1 and 6.5 g L?1 of urea, at 30 ?C and 150 rpm. Crude glycerol proved to be an excellent substrate option for SCP production, adding value to this residue. W. anomalus CCC32 showed promising characteristics for SCP production as fast growth and biomass production with high crude protein content.Downloads
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References
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GALANAKIS, C. M. Recovery of high components from food wastes: conventional, emerging technologies and commercialized applications. Trends in Food Science Technology, Cambridge, v. 26, p. 68-87, 2012.
GALVAGNO, M. A.; IANNONE, L. J.; BIANCHI, J.; KRONBERG, F.; ROST, E.; CARSTENS, M. R.; CERRUTTI, P. Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol. Revista Argentina de Microbiologia, Buenos Aires, v. 43, p. 218-225, 2011.
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OLSTORPE, M.; BORLING, J.; SCHNÜRER, J.; PASSOTH, V. Pichia anomala yeast improves feed hygiene during storage of moist crimped barley grain under Swedish farm conditions. Animal Feed Science Technology, Amsterdam, v. 156, p. 47-56, 2010.
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PATELSKI, P.; BERLOWSKA, J.; DZIUGAN, P; PIELECH-PRZYBYLSKA, K.; BALCEREK, M.; DZIEKONSKA, U.; KALINOWSKA, H. Utilization of sugar beet bagasse for the biosynthesis of yeast SCP. Journal of Food Engineering, Essex, v. 167, p. 32-37, 2015.
PONSANO, E. H. G.; LACAVA, P. M.; PINTO, M. F. Chemical composition of Rhodocyclus gelatinosus biomass produced in poultry slaughterhouse wastewater. Brazilian Archives of Biology and Technology, Curitiba, v. 46, p. 143-147, 2003. https://doi: 143-147.
QIN, L.; LIU, L.; ZENG, A-P.; WEI, D. From low-cost substrates to single cell oils synthesized by oleaginous yeasts. Bioresource Technology, Barking, v. 245, p. 1507- 1519, 2017.
RODRIGUES, M. I.; IEMMA, A. F. Planejamento de experimentos e otimização de processos. 2. ed. Brasil: Cárita, 2009.
RITALA, A.; RITALA, A.; HÄKKINEN, S. T.; TOIVARI, M.; WIEBE, M. G. Single cell protein-state-of-the-art, industrial landscape and patents 2001-2016. Frontiers in Microbiology, Lausanne, v. 8, p. 1-18, 2016.
SANTOS, E. O.; MICHELON, M.; FURLONG, E. B.; DE MEDEIROS BURKERT, J. F.; KALIL, S. J.; BURKERT, C.A.V. Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis. Brazilian Journal of Microbiology, Rio de Janeiro, v. 1, p. 432-440, 2012.
SANTOS, E. O.; MICHELON, M.; GALLAS, J. A.; KALIL, S. J.; BURKERT, C. A. J. Raw glycerol as substrate for the production of yeast biomass. Internationl Journal of Food Engineering, Ghaziabad, v. 9, p. 413-420, 2013.
SILVA, G. P.; LIMA, C.; CONTIERO. Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29. Catalisis Today, Amsterdam, v. 257, p. 259-266, 2015.
SISMAN, T.; GUR, O.; DOGAN, N.; OZDAL, M.; ALGUR, O. F.; ERGON, T. Single-cell protein as an alternative food for zebrafish, Danio rerio: a toxicological assessment. Toxicology and Industrial Health, London, v. 29, p. 792-799, 2012.
SOUZA, K. S. T.; GUDINA, E. J.; SCHWAN, R. F.; RODRIGUES, L. R.; DIAS, D. R.; TEIXEIRA, J. A. Improvement of biosurfactant production by Wickerhamomyces anomalus CCMA 0358 and its potential application in bioremediation. Journal Hazard Material, Buffalo, v. 346, p. 152-158, 2018.
SUNDH, I.; MELIN, P. Safety and regulation of yeasts intentionally added to the food or feed chains. Antonie Van Leeuwenhoek, Amsterdam, v. 99, p. 113-119, 2010.
TACCARI, M.; CANONICO, L.; COMITINI, F.; MANNAZZU, I.; CIANI, M. Screening of yeasts for growth on crude glycerol and optimization of biomass production. Bioresource Technology, Essex, v. 110, p. 488-495, 2012.
ALI, S.; AHMED, S.; SHEIKH, M. A.; HASHMI, S. A.; RAJOKA, M. B.; JAMIL, A. Lysine production by Lhomoserine resistant mutant of Brevibacterium flavum. Journal of The Chemical Society of Pakistan, Pakistan, v. 32, p. 97-91, 2009.
ANAPUMA; RAVINDRA, P. Value-added food: single cell protein. Bioresource Technology, New Yourk, v. 18, p. 459-479, 2000.
BZDUCHA-WROBEL, A.; BŁAZEJAK, S.; MOLENDA, M.; RECZEK, L. Biosynthesis of β (1,3)/(1,6)- glucans of cell wall of the yeast Candida utilis ATCC 9950 strains in the culture media supplemented with deproteinated potato juice water and glycerol. European Food Research Technology, Berlin, v. 240, n. 5, p. 1023-1034, 2015.
BASSO, R. F.; ALCARDE, A. R.; PORTUGAL, C. B. Could non-Saccharomyces yeasts contribute on innovative brewing fermentations?. Food Research International, Barking, v. 86, p. 112-120, 2006.
CAPPELLI, A.; ULISSI, U.; VALZANO, M.; DAMIANI, C.; EPIS, S.; GABRIELLI, M. G.; CONTI, S.; POLONELLI, L.; BANDI, C.; FAVIA, G.; RICCI, I. A Wickerhamomyces anomalus killer strain in the Malaria vector Anopheles stephensi. Plos One, San Francisco, v. 9, p. 1-9, 2014.
DUARTE, S.H.; GHISELLI, G.; MAUGERI, F. Influence of culture conditions on lipid production by Candida sp. LEB-M3 using glycerol from biodiesel synthesis. Biocatalysis and Agricultural Biotechnology, v. 2, p. 339–343, 2013.
FELKER, P. Microdetermination of nitrogen in seed protein extracts. Analytical Chemistry, Amsterdam, p. 49, v. 1080-1080, 1977.
GALANAKIS, C. M. Recovery of high components from food wastes: conventional, emerging technologies and commercialized applications. Trends in Food Science Technology, Cambridge, v. 26, p. 68-87, 2012.
GALVAGNO, M. A.; IANNONE, L. J.; BIANCHI, J.; KRONBERG, F.; ROST, E.; CARSTENS, M. R.; CERRUTTI, P. Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol. Revista Argentina de Microbiologia, Buenos Aires, v. 43, p. 218-225, 2011.
GAO, L.; CHI, Z.; SHENG, J.; NI, X.; WANG, L. Singlecell protein production from Jerusalem artichoke extract by a recently isolated marine yeast Cryptococcus aureus G7a and its nutritive analysis. Applied Microbiology Biotechnology, Berlin, v. 77, p. 825-832, 2007.
GAO, Y.; LI, D.; LIU, Y. Production of single cell protein from soy molasses using Candida tropicalis. Annals of Microbiology, Milano, v. 62, p. 1165-1172, 2012.
JUSZCZYK, P.; TOMASZEWSKA, L.; KITA, A.; RYMOWICZ, W. Biomass production by novel strains of Yarrowia lipolytica using raw glycerol, derived from biodiesel production. Bioresourcce Technology, Amsterdam, v. 137, p. 124-131, 2013.
KONÉ, M. K.; GUÉHI, S. T.; DURAND, N.; BANKOFFI, L.; BERTHIOT, L.; TACHON, A. F.; BROU, K.; BOULANGER, R.; MONTET, D. Contribution of predominant yeasts to the occurrence of aroma compounds during cocoa bean fermentation. Food Research International, Ottawa, v. 89, p. 910-917, 2016.
KURCZ, A.; BŁAZEJAK, S.; KOT, A. M.; BZDUCHAWROBEL, A.; KIELISZEK, M. Application of industrial wastes for the production of microbial Single-Cell Protein by fodder yeast Candida utilis. Waste and Biomass Valorization, Netherlands, v. 9, p. 57-64, 2018.
LI, M.; LIAO, X.; ZHANG, D.; DU, G.; CHEN, J. Yeast extract promotes cell growth and induces production of polyvinyl alcohol-degrading enzymes. Enzyme Research, New York, v. 2011, p. 1-8, 2011.
LI, Z.; WANG, D.; SHI, Y.C. Effects of nitrogen source on ethanol production in very high gravity fermentation of corn starch. Journal of Taiwan Institute Chemical Engineers, Amsterdam, v. 70, p. 229-235, 2017.
LUO, X.; GE, X.; CUI, S.; LI, Y. Value-added processing of crude glycerol into chemicals and polymers. Bioresource Technology, Barking, v. 215, p. 144-154, 2016 .
NAJAFPOUR, G. D. Single cell protein. In: NAJAFPOUR, G. D. Biochemical engineering and biotechnology. 2. ed. Nova York: Elsevier Science, 2015. p. 417-434.
OJHAA, N.; DAS, N. A Statistical approach to optimize the production of polyhydroxyalkanoates from Wickerhamomyces anomalus VIT-NN01 using response surface methodology. International Journal of Biology Macromolecules, Colorado, v. 107, p. 2157-2170, 2018.
OLSTORPE, M.; BORLING, J.; SCHNÜRER, J.; PASSOTH, V. Pichia anomala yeast improves feed hygiene during storage of moist crimped barley grain under Swedish farm conditions. Animal Feed Science Technology, Amsterdam, v. 156, p. 47-56, 2010.
ORO, L.; ERICA, F.; CIANIA, M.; ROMANAZZIB, G.; COMITINIA, F. Biocontrol of postharvest brown rot of sweet cherries by Saccharomyces cerevisiae Disva 599, Metschnikowia pulcherrima Disva 267 and Wickerhamomyces anomalus Disva 2 strains. Postharvest Biology and Technology, Amsterdam, v. 96, p. 64-68, 2014.
PAPANIKOLAOU, S.; FAKAS, S.; FICK, M.; CHEVALOT, I.; GALIOTOU-PANAYOTOU, M.; KOMAITIS, M.; IVAN MARC, I.; AGGELIS, G. Biotechnological valorization of raw glycerol discharged after biodiesel (fatty acid methyl esters) manufacturing process: production of 1,3-propanediol, citric acid and single cell oil. Biomass and Bionergy, Oxford, v. 32, p. 60-71, 2008.
PATELSKI, P.; BERLOWSKA, J.; DZIUGAN, P; PIELECH-PRZYBYLSKA, K.; BALCEREK, M.; DZIEKONSKA, U.; KALINOWSKA, H. Utilization of sugar beet bagasse for the biosynthesis of yeast SCP. Journal of Food Engineering, Essex, v. 167, p. 32-37, 2015.
PONSANO, E. H. G.; LACAVA, P. M.; PINTO, M. F. Chemical composition of Rhodocyclus gelatinosus biomass produced in poultry slaughterhouse wastewater. Brazilian Archives of Biology and Technology, Curitiba, v. 46, p. 143-147, 2003. https://doi: 143-147.
QIN, L.; LIU, L.; ZENG, A-P.; WEI, D. From low-cost substrates to single cell oils synthesized by oleaginous yeasts. Bioresource Technology, Barking, v. 245, p. 1507- 1519, 2017.
RODRIGUES, M. I.; IEMMA, A. F. Planejamento de experimentos e otimização de processos. 2. ed. Brasil: Cárita, 2009.
RITALA, A.; RITALA, A.; HÄKKINEN, S. T.; TOIVARI, M.; WIEBE, M. G. Single cell protein-state-of-the-art, industrial landscape and patents 2001-2016. Frontiers in Microbiology, Lausanne, v. 8, p. 1-18, 2016.
SANTOS, E. O.; MICHELON, M.; FURLONG, E. B.; DE MEDEIROS BURKERT, J. F.; KALIL, S. J.; BURKERT, C.A.V. Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis. Brazilian Journal of Microbiology, Rio de Janeiro, v. 1, p. 432-440, 2012.
SANTOS, E. O.; MICHELON, M.; GALLAS, J. A.; KALIL, S. J.; BURKERT, C. A. J. Raw glycerol as substrate for the production of yeast biomass. Internationl Journal of Food Engineering, Ghaziabad, v. 9, p. 413-420, 2013.
SILVA, G. P.; LIMA, C.; CONTIERO. Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29. Catalisis Today, Amsterdam, v. 257, p. 259-266, 2015.
SISMAN, T.; GUR, O.; DOGAN, N.; OZDAL, M.; ALGUR, O. F.; ERGON, T. Single-cell protein as an alternative food for zebrafish, Danio rerio: a toxicological assessment. Toxicology and Industrial Health, London, v. 29, p. 792-799, 2012.
SOUZA, K. S. T.; GUDINA, E. J.; SCHWAN, R. F.; RODRIGUES, L. R.; DIAS, D. R.; TEIXEIRA, J. A. Improvement of biosurfactant production by Wickerhamomyces anomalus CCMA 0358 and its potential application in bioremediation. Journal Hazard Material, Buffalo, v. 346, p. 152-158, 2018.
SUNDH, I.; MELIN, P. Safety and regulation of yeasts intentionally added to the food or feed chains. Antonie Van Leeuwenhoek, Amsterdam, v. 99, p. 113-119, 2010.
TACCARI, M.; CANONICO, L.; COMITINI, F.; MANNAZZU, I.; CIANI, M. Screening of yeasts for growth on crude glycerol and optimization of biomass production. Bioresource Technology, Essex, v. 110, p. 488-495, 2012.
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Published
2019-12-18
How to Cite
Silva, J. P. da, da Silva, G. P. da, Azevedo Neto, A. D. de, & Cazetta, M. L. (2019). Single cell protein production by Wickerhamomyces anomalus CCC32 using crude glycerol. Semina: Ciências Exatas E Tecnológicas, 40(2), 179–188. https://doi.org/10.5433/1679-0375.2019v40n2p179
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