Palavras-chave
Eimeriidae
Coccidiose
PCR
Columbiformes
Citocromo c oxidase I gene.
Coccidiose
PCR
Columbiformes
Citocromo c oxidase I gene.
Como Citar
Taroda, A., Barros, L. D. de, Seixas, M. de, Cardim, S. T., Sasse, J. P., Minutti, A. F., … Garcia, J. L. (2020). Primeira detecção molecular de Eimeria spp. em pombos (Zenaida auriculata) do Brasil. Semina: Ciências Agrárias, 41(4), 1259–1266. https://doi.org/10.5433/1679-0359.2020v41n4p1259
Resumo
O objetivo do presente estudo foi detectar Eimeria spp. em pombos (Zenaida auriculata) do Brasil. Duzentos e quatorze pombos foram capturados em diferentes regiões da cidade de Londrina, estado do Paraná, Sul do Brasil. Amostras fecais foram coletadas e realizada a extração de DNA. Uma nested-PCR baseada na subunidade I do gene oxidase do citocromo c oxidase do genoma mitocondrial de Eimeria spp. foi utilizada para detectar a presença de DNA deste coccídeo nas amostras fecais dos pombos. Das 214 aves, 171 (79,9%) foram positivas para Eimeria spp. Houve uma diferença significativa de positividade entre o local de captura. Quando analisado o gênero, o número de machos positivos foi 84/103 (81,5%) e fêmeas 87/111 (78,4%). Para o conhecimento, este é o primeiro estudo com prevalência molecular de Eimeria spp. em Z. auriculata. Novos estudos devem ser conduzidos para identificar as espécies de Eimeria que infectam pombos Z. auriculata.Referências
Adriano, E. A., & Cordeiro, N. S. (2001). Prevalence and intensity of Haemoproteus columbae in three species of wild doves from Brazil. Memórias do Instituto Oswaldo Cruz, 96(2), 175-178. doi: 10.1590/S0074-02762001000200007
Adriano, E. A., Thyssen, P. J., & Cordeiro, N. S. (2000). Eimeria curvata n. sp. (Apicomplexa: Eimeriidae) in Columbina talpacoti and Scardafella squammata (Aves: Columbidae) from Brazil. Memorias do Instituto Oswaldo Cruz, 95(1), 53-55. doi: 10.1590/S0074-02762000000100008
Adriano, E. A., Thyssen, P. J., & Cordeiro, N. S. (2003). A new species of Eimeria from the eared dove Zenaida auriculata (Aves: Columbidae) in Brazil. Acta Protozoologica, 42(1), 71-73.
Aleksandra, B., & Pilarczyk, B. (2014). Occurrence of coccidia infection in pigeons in amateur husbandry. Diagnosis and prevention. Annals of Parasitology, 60(2), 93-97. PMID: 25115060
Atkinson, C. T., Thomas, N. J., & Hunter, D. B. (2009). Parasitic diseases of wild birds. In Parasitic Diseases of Wild Birds. Wiley-Blackwell. doi: 10.1002/9780813804620
Ball, S. J., Daszak, P., Swinnerton, K. R., Jones, C. G., & Snow, K. R. (2012). A new species of Eimeria (Apicomplexa: Eimeriidae) from the endangered pink pigeon, Nesoenas mayeri (Prévost, 1843) Cheke, 2005 (Columbiformes) in Mauritius. African Zoology, 47(2), 369-372. doi: 10.3377/004.047.0203
Berto, B. P., McIntosh, D., & Lopes, C. W. G. (2014). Studies on coccidian oocysts (Apicomplexa: Eucoccidiorida). Revista Brasileira de Parasitologia Veterinária, 23(1), 1-15. doi: 10.1590/S1984-29612014001
Cardozo, S. P., & Yamamura, M. H. (2004). Parasitas em produção de fragos no sistema de criação tipo colonial/caipira no Brasil. Semina: Ciências Agrárias, 25(1), 63-74. doi: 10.5433/1679-0359.2004v25n1p63
Carvalho, F. S., Wenceslau, A. A., Teixeira, M., Matos Carneiro, J. A., Melo, A. D. B., & Albuquerque, G. R. (2011). Diagnosis of Eimeria species using traditional and molecular methods in field studies. Veterinary Parasitology, 176(2-3), 95-100. doi: 10.1016/j.vetpar.2010.11.015
Cole, R. A., & Friend, M. (1999). Intestinal Coccidiosis. In M. Friend & J. C. Franson (Ed.), Field manual of wildlife diseases (Chapter 26, pp. 207-213). Madison: National Wildlife Health Center.
Conti, J. A., & Forrester, D. J. (1981). Interrelationships of parasites of white-winged doves and mouring dobes in Florida. Journal of Wildlife Diseases, 17(4), 529-536. doi: 10.7589/0090-3558-17.4.529
Dean, A. G., Dean, J. A., Coulombier, D., Brendel, K. A., Smith, D. C., Burton, D. H., Dicker, R. C.,... Arner, T. G. (1994) EpiInfo, Version 6: A word Processing, Database, and Statistic Program for Epidemiology on Microcomputers. Georgia: Center for Diseases Control and Prevention.
Dolnik, O. V., Palinauskas, V., & Bensch, S. (2009). Individual oocysts of Isospora (Apicomplexa: Coccidia) parasites from avian feces: From photo to sequence. Journal of Parasitology, 95(1), 169-174. doi: 10.1645/GE-1873.1
González, D., Daugschies, A., Rubilar, L., Pohlmeyer, K., Skewes, O., & Mey, E. (2004). Fauna parasitaria de la tórtola común (Zenaida auriculata, de Murs 1847) (Columbiformes: Columbidae) en Ñuble, Chile. Parasitología Latinoamericana, 59(1-2), 37-41. doi: 10.4067/S0717-77122004000100007
Hafeez, M. A., Shivaramaiah, S., Dorsey, K. M., Ogedengbe, M. E., El-Sherry, S., Whale, J.,… Barta, J. R. (2015). Simultaneous identification and DNA barcoding of six Eimeria species infecting turkeys using PCR primers targeting the mitochondrial cytochrome c oxidase subunit I (mtCOI) locus. Parasitology Research, 114(5), 1761-1768. doi: 10.1007/s00436-015-4361-y
Jamriška, J., & Modrý, D. (2012). A new species of Eimeria Schneider, 1875 (Apicomplexa: Eimeriidae) from the common wood pigeon Columba palumbus Linnaeus, 1758 (Aves: Columbidae). Acta Protozoologica, 51(4), 329-333. doi: 10.4467/16890027AP.12.026.0786
Marques, S. M. T., de Cuadros, R. M., Silva, C. J. da, & Baldo, M. (2007). Parasites of pigeons (Columba livia) in urban areas of lages, Southern Brazil. Parasitología Latinoamericana, 62(3-4), 183-187. doi: 10.4067/S0717-77122007000200014
McQuistion, T. E. (1991). Eimeria palumbi, a new coccidian parasite (Apicomplexa: Eimeriidae) from the galapagos dove (Zenaida galapogoensis). Transactions of the American Microscopal Society, 110(2), 178-181. doi: 10.2307/3226755
Natala, A. J., Asemadahun, N. D., Okubanjo, O. O., Ulayi, B. M., Owolabi, Y. H., Jato, I. D., & Yusuf, K. H. (2009). A survey of parasites of domesticated pigeon (Columba livia domestica) in Zaria, Nigeria. International Journal of Soft Computing, 4(4), 148-150. doi: ijscomp.2009.148.150
Ogedengbe, J. D., Hanner, R. H., & Barta, J. R. (2011). DNA barcoding identifies Eimeria species and contributes to the phylogenetics of coccidian parasites (Eimeriorina, Apicomplexa, Alveolata). International Journal for Parasitology, 41(8), 843-850. doi: 10.1016/j.ijpara.2011.03.007
Pérez-Tris, J., & Bensch, S. (2005). Diagnosing genetically diverse avian malarial infections using mixed-sequence analysis and TA-cloning. Parasitology, 131(1), 15-23. doi: 10.1017/S003118200500733X
Radfar, M. H., Fathi, S., Asl, E. N., Dehaghi, M. M., & Seghinsara, H. R. (2011). A survey of parasites of domestic pigeons (Columba livia domestica) in South Khorasan, Iran. Veterinary Research, 4(1), 18-23. doi: 10.3923/vr.2011.18.23
Rathinam, T., Gadde, U., & Chapman, H. D. (2015). Molecular detection of field isolates of Turkey Eimeria by polymerase chain reaction amplification of the cytochrome c oxidase I gene. Parasitology Research, 114(7), 2795-2799. doi: 10.1007/s00436-015-4546-4
Reid, A. J., Blake, D. P., Ansari, H. R., Billington, K., Browne, H. P., Bryant, J.,… Pain, A. (2014). Genomic analysis of the causative agents of coccidiosis in domestic chickens. Genome Research, 24(10), 1676-1685. doi: 10.1101/gr.168955.113
Sari, B., Karatepe, B., Karatepe, M., & Kara, M. (2008). Parasites of domestic (Columba livia domestica) and wild (Columba livia livia) pigeons in nigde, Turkey. Bulletin of the Veterinary Institute in Pulawy, 52(4), 551-554.
Shibatta, O. A., Galves, W., Carmo, W. P. D. do, Lima, I. P. de, Lopes, E. V., & Machado, R. A. (2009). A fauna de vertebrados do campus da Universidade Estadual de Londrina, região norte do estado do Paraná, Brasil. Semina: Ciências Biológicas e da Saúde, 30(1), 3-26. doi: 10.5433/1679-0367.2009v30n1p3
Valkiūnas, G., Bensch, S., Iezhova, T. A., Križanauskienė, A., Hellgren, O., & Bolshakov, C. V. (2006). Nested cytochrome B polymerase chain reaction diagnostics underestimate mixed infections of avian blood haemosporidian parasites: microscopy is still essential. Journal of Parasitology, 92(2), 418-422. doi: 10.1645/GE-3547RN.1
Valkiūnas, G., Iezhova, T. A., Križanauskienė, A., Palinauskas, V., Sehgal, R. N. M., & Bensch, S. (2008). A Comparative analysis of microscopy and PCR-based detection methods for blood parasites. Journal of Parasitology, 94(6), 1395-1401. doi: 10.1645/GE-1570.1
Yang, R., Brice, B., Elloit, A., Lee, E., & Ryan, U. (2014). Morphological and molecular characterization of Eimeria paludosa coccidian parasite (Apicomplexa:Eimeriidae) in a dusky moorhen (Gallinula tenebrosa, Gould, 1846) in Australia. Experimental Parasitology, 147, 16-22. doi: 10.1016/j.exppara.2014.10.010
Yang, R., Brice, B., Elloit, A., & Ryan, U. (2016a). Morphological and molecular characterization of Eimeria labbeana-like (Apicomplexa:Eimeriidae) in a domestic pigeon (Columba livia domestica, Gmelin, 1789) in Australia. Experimental Parasitology, 166, 124-130. doi: 10.1016/j.exppara.2016.04.009
Yang, R., Brice, B., & Ryan, U. (2016b). Morphological and molecular characterization of Eimeria purpureicephali n. sp. (Apicomplexa:Eimeriidae) in a red-capped parrot (Purpureicephalus spurius, Kuhl, 1820) in Western Australia. International Journal for Parasitology: Parasites and Wildlife, 5(1), 34-39. doi: 10.1016/j.ijppaw.2016.01.003
Adriano, E. A., Thyssen, P. J., & Cordeiro, N. S. (2000). Eimeria curvata n. sp. (Apicomplexa: Eimeriidae) in Columbina talpacoti and Scardafella squammata (Aves: Columbidae) from Brazil. Memorias do Instituto Oswaldo Cruz, 95(1), 53-55. doi: 10.1590/S0074-02762000000100008
Adriano, E. A., Thyssen, P. J., & Cordeiro, N. S. (2003). A new species of Eimeria from the eared dove Zenaida auriculata (Aves: Columbidae) in Brazil. Acta Protozoologica, 42(1), 71-73.
Aleksandra, B., & Pilarczyk, B. (2014). Occurrence of coccidia infection in pigeons in amateur husbandry. Diagnosis and prevention. Annals of Parasitology, 60(2), 93-97. PMID: 25115060
Atkinson, C. T., Thomas, N. J., & Hunter, D. B. (2009). Parasitic diseases of wild birds. In Parasitic Diseases of Wild Birds. Wiley-Blackwell. doi: 10.1002/9780813804620
Ball, S. J., Daszak, P., Swinnerton, K. R., Jones, C. G., & Snow, K. R. (2012). A new species of Eimeria (Apicomplexa: Eimeriidae) from the endangered pink pigeon, Nesoenas mayeri (Prévost, 1843) Cheke, 2005 (Columbiformes) in Mauritius. African Zoology, 47(2), 369-372. doi: 10.3377/004.047.0203
Berto, B. P., McIntosh, D., & Lopes, C. W. G. (2014). Studies on coccidian oocysts (Apicomplexa: Eucoccidiorida). Revista Brasileira de Parasitologia Veterinária, 23(1), 1-15. doi: 10.1590/S1984-29612014001
Cardozo, S. P., & Yamamura, M. H. (2004). Parasitas em produção de fragos no sistema de criação tipo colonial/caipira no Brasil. Semina: Ciências Agrárias, 25(1), 63-74. doi: 10.5433/1679-0359.2004v25n1p63
Carvalho, F. S., Wenceslau, A. A., Teixeira, M., Matos Carneiro, J. A., Melo, A. D. B., & Albuquerque, G. R. (2011). Diagnosis of Eimeria species using traditional and molecular methods in field studies. Veterinary Parasitology, 176(2-3), 95-100. doi: 10.1016/j.vetpar.2010.11.015
Cole, R. A., & Friend, M. (1999). Intestinal Coccidiosis. In M. Friend & J. C. Franson (Ed.), Field manual of wildlife diseases (Chapter 26, pp. 207-213). Madison: National Wildlife Health Center.
Conti, J. A., & Forrester, D. J. (1981). Interrelationships of parasites of white-winged doves and mouring dobes in Florida. Journal of Wildlife Diseases, 17(4), 529-536. doi: 10.7589/0090-3558-17.4.529
Dean, A. G., Dean, J. A., Coulombier, D., Brendel, K. A., Smith, D. C., Burton, D. H., Dicker, R. C.,... Arner, T. G. (1994) EpiInfo, Version 6: A word Processing, Database, and Statistic Program for Epidemiology on Microcomputers. Georgia: Center for Diseases Control and Prevention.
Dolnik, O. V., Palinauskas, V., & Bensch, S. (2009). Individual oocysts of Isospora (Apicomplexa: Coccidia) parasites from avian feces: From photo to sequence. Journal of Parasitology, 95(1), 169-174. doi: 10.1645/GE-1873.1
González, D., Daugschies, A., Rubilar, L., Pohlmeyer, K., Skewes, O., & Mey, E. (2004). Fauna parasitaria de la tórtola común (Zenaida auriculata, de Murs 1847) (Columbiformes: Columbidae) en Ñuble, Chile. Parasitología Latinoamericana, 59(1-2), 37-41. doi: 10.4067/S0717-77122004000100007
Hafeez, M. A., Shivaramaiah, S., Dorsey, K. M., Ogedengbe, M. E., El-Sherry, S., Whale, J.,… Barta, J. R. (2015). Simultaneous identification and DNA barcoding of six Eimeria species infecting turkeys using PCR primers targeting the mitochondrial cytochrome c oxidase subunit I (mtCOI) locus. Parasitology Research, 114(5), 1761-1768. doi: 10.1007/s00436-015-4361-y
Jamriška, J., & Modrý, D. (2012). A new species of Eimeria Schneider, 1875 (Apicomplexa: Eimeriidae) from the common wood pigeon Columba palumbus Linnaeus, 1758 (Aves: Columbidae). Acta Protozoologica, 51(4), 329-333. doi: 10.4467/16890027AP.12.026.0786
Marques, S. M. T., de Cuadros, R. M., Silva, C. J. da, & Baldo, M. (2007). Parasites of pigeons (Columba livia) in urban areas of lages, Southern Brazil. Parasitología Latinoamericana, 62(3-4), 183-187. doi: 10.4067/S0717-77122007000200014
McQuistion, T. E. (1991). Eimeria palumbi, a new coccidian parasite (Apicomplexa: Eimeriidae) from the galapagos dove (Zenaida galapogoensis). Transactions of the American Microscopal Society, 110(2), 178-181. doi: 10.2307/3226755
Natala, A. J., Asemadahun, N. D., Okubanjo, O. O., Ulayi, B. M., Owolabi, Y. H., Jato, I. D., & Yusuf, K. H. (2009). A survey of parasites of domesticated pigeon (Columba livia domestica) in Zaria, Nigeria. International Journal of Soft Computing, 4(4), 148-150. doi: ijscomp.2009.148.150
Ogedengbe, J. D., Hanner, R. H., & Barta, J. R. (2011). DNA barcoding identifies Eimeria species and contributes to the phylogenetics of coccidian parasites (Eimeriorina, Apicomplexa, Alveolata). International Journal for Parasitology, 41(8), 843-850. doi: 10.1016/j.ijpara.2011.03.007
Pérez-Tris, J., & Bensch, S. (2005). Diagnosing genetically diverse avian malarial infections using mixed-sequence analysis and TA-cloning. Parasitology, 131(1), 15-23. doi: 10.1017/S003118200500733X
Radfar, M. H., Fathi, S., Asl, E. N., Dehaghi, M. M., & Seghinsara, H. R. (2011). A survey of parasites of domestic pigeons (Columba livia domestica) in South Khorasan, Iran. Veterinary Research, 4(1), 18-23. doi: 10.3923/vr.2011.18.23
Rathinam, T., Gadde, U., & Chapman, H. D. (2015). Molecular detection of field isolates of Turkey Eimeria by polymerase chain reaction amplification of the cytochrome c oxidase I gene. Parasitology Research, 114(7), 2795-2799. doi: 10.1007/s00436-015-4546-4
Reid, A. J., Blake, D. P., Ansari, H. R., Billington, K., Browne, H. P., Bryant, J.,… Pain, A. (2014). Genomic analysis of the causative agents of coccidiosis in domestic chickens. Genome Research, 24(10), 1676-1685. doi: 10.1101/gr.168955.113
Sari, B., Karatepe, B., Karatepe, M., & Kara, M. (2008). Parasites of domestic (Columba livia domestica) and wild (Columba livia livia) pigeons in nigde, Turkey. Bulletin of the Veterinary Institute in Pulawy, 52(4), 551-554.
Shibatta, O. A., Galves, W., Carmo, W. P. D. do, Lima, I. P. de, Lopes, E. V., & Machado, R. A. (2009). A fauna de vertebrados do campus da Universidade Estadual de Londrina, região norte do estado do Paraná, Brasil. Semina: Ciências Biológicas e da Saúde, 30(1), 3-26. doi: 10.5433/1679-0367.2009v30n1p3
Valkiūnas, G., Bensch, S., Iezhova, T. A., Križanauskienė, A., Hellgren, O., & Bolshakov, C. V. (2006). Nested cytochrome B polymerase chain reaction diagnostics underestimate mixed infections of avian blood haemosporidian parasites: microscopy is still essential. Journal of Parasitology, 92(2), 418-422. doi: 10.1645/GE-3547RN.1
Valkiūnas, G., Iezhova, T. A., Križanauskienė, A., Palinauskas, V., Sehgal, R. N. M., & Bensch, S. (2008). A Comparative analysis of microscopy and PCR-based detection methods for blood parasites. Journal of Parasitology, 94(6), 1395-1401. doi: 10.1645/GE-1570.1
Yang, R., Brice, B., Elloit, A., Lee, E., & Ryan, U. (2014). Morphological and molecular characterization of Eimeria paludosa coccidian parasite (Apicomplexa:Eimeriidae) in a dusky moorhen (Gallinula tenebrosa, Gould, 1846) in Australia. Experimental Parasitology, 147, 16-22. doi: 10.1016/j.exppara.2014.10.010
Yang, R., Brice, B., Elloit, A., & Ryan, U. (2016a). Morphological and molecular characterization of Eimeria labbeana-like (Apicomplexa:Eimeriidae) in a domestic pigeon (Columba livia domestica, Gmelin, 1789) in Australia. Experimental Parasitology, 166, 124-130. doi: 10.1016/j.exppara.2016.04.009
Yang, R., Brice, B., & Ryan, U. (2016b). Morphological and molecular characterization of Eimeria purpureicephali n. sp. (Apicomplexa:Eimeriidae) in a red-capped parrot (Purpureicephalus spurius, Kuhl, 1820) in Western Australia. International Journal for Parasitology: Parasites and Wildlife, 5(1), 34-39. doi: 10.1016/j.ijppaw.2016.01.003
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