Comparison between vitrification and slow freezing on the post-thaw development of bovine embryos produced in vitro

Authors

DOI:

https://doi.org/10.5433/1679-0359.2024v44n2p537

Keywords:

Cryosurvival, Direct Transfer, IVEP.

Abstract

We evaluated the post-thaw development of in vitro-produced (IVP) bovine embryos cryopreserved using vitrification and slow-freezing techniques on different days after in vitro fertilization (IVF). Nine replicates of IVP were performed. Embryos at the expanded blastocyst stage with quality grades 1 and 2 (according to the International Embryo Technology Society (IETS) manual) were selected on days 7, 7.5, and 8 after IVF. Embryos (n = 472) were randomly divided and cryopreserved using slow freezing (n = 257) or vitrification (n = 215). The embryos were organized into six groups according to the cryopreservation technique and the day: 1) Group DT7 (embryos subjected to slow freezing on D7, n = 140); 2) Group DT7.5 (embryos subjected to slow freezing 12 hours after D7, on D7.5; n = 61); 3) Group DT8 (embryos subjected to slow freezing on D8, n = 56); 4) Group VIT7 (embryos vitrified on D7, n = 127); 5) Group VIT7.5 (embryos vitrified 12 hours after D7, on D7.5; n = 49); and 6) Group VIT8 (embryos vitrified on D8, n = 39). Data were arcsine-transformed and analyzed using analysis of variance and the GLIMMIX procedure in SAS (SAS 9.2), with P < 0.05. The re-expansion and embryo hatching rates were higher in vitrified embryos than in embryos subjected to slow freezing (P < 0.05). Embryo quality grade did not influence the total developmental rate (P > 0.05). However, grade 1 embryos re-expanded more rapidly (within 24 hours) than grade 2 embryos. Grade 1 embryos showed better results with vitrification than with slow freezing. The experimental groups represented a technique × day interaction and did not differ in terms of re-expansion and hatching rates (P > 0.05).

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Author Biographies

Vanessa Rachele Ribeiro Nunes, Universidade Federal do Acre

Student of the Master's Course of the Postgraduate Program in Health and Sustainable Animal Production in the Western Amazon, Universidade Federal do Acre, UFAC, Rio Branco, AC, Brazil.

Jennifer Teodoro Ferreira Gregianini, Universidade Federal do Acre

Student of the Master's Course of the Postgraduate Program in Health and Sustainable Animal Production in the Western Amazon, Universidade Federal do Acre, UFAC, Rio Branco, AC, Brazil.

Helton Aparecido Garcia Gregianini, Empresa In vitro Acre

 M.e, Embryo Production Director , Company In vitro Acre, Rio Branco, AC, Brazil.

Bárbara Souza Fantin, Empresa In vitro Acre

Graduate in Veterinary Medicine, Laboratory Technician, Company In vitro Acre, Rio Branco, AC, Brazil.

Bárbara Loureiro, Universidade de Vila Velha

Profa. Dra., Laboratory of Animal Reproductive Physiology, Universidade de Vila Velha, UVV, Vila Velha, Espírito Santo, Brazil.

Rafael Augusto Satrapa, Universidade Federal do Acre

Prof. Dr., Center for Biological and Natural Sciences, Universidade Federal do Acre, UFAC, Rio Branco, Acre, Brazil.

References

Aksu, D. A., Agca, C., Aksu, S., Bagis, H., Akkoc, T., Caputcu, A. T., Arat, S., Taskin, A. C., Kizil, S. H., & Karasahin, T. (2012). Gene expression profiles of vitrified in vitro and in vivo-derived bovine blastocysts. Molecular Reproduction and Development, 79(9), 613-625. doi: 10.1002/mrd.22068 DOI: https://doi.org/10.1002/mrd.22068

Almiñana, C., & Cuello, C. (2015). What is new in the cryopreservation of embryos? Animal Reproduction, 12(3), 418-427. https://www.animal-reproduction.org/article/5b5a6031f7783717068b460b/pdf/animreprod-12-3-418.pdf

Amstislavsky, S., Mokrousova, V., Brusentsev, E., Okotrub, K., & Comizzoli, P. (2019). Influence of cellular lipids on cryopreservation of mammalian oocytes and preimplantation embryos: a review. Biopreservation and Biobanking, 17(1), 76-83. doi: 10.1089/bio.2018.0039 DOI: https://doi.org/10.1089/bio.2018.0039

Arshad, U., Sagheer, M., Gonzalez-Silvestry, F. B., Hassan, M., & Sosa, F. (2021). Vitrification improves in-vitro embryonic survival in Bos taurus embryos without increasing pregnancy rate post embryo transfer when compared to slow-freezing: a systematic meta-analysis. Cryobiology, 101(8), 1-11. doi: 10.1016/j.cryobiol.2021.06.007 DOI: https://doi.org/10.1016/j.cryobiol.2021.06.007

Barceló-Fimbres, M., & Seidel, Jr., G. E. (2007). Effects of fetal calf serum, phenazine ethosulfate and either glucose or fructose during in vitro culture of bovine embryos on embryonic development after cryopreservation. Molecular Reproduction and Development, 74(11), 1395-1405. doi: 10.1002/mrd.20699 DOI: https://doi.org/10.1002/mrd.20699

Bó, G. A., & Mapletoft, R. J. (2013). Evaluation and classification of bovine embryos. Animal Reproduction, 10(3), 344-348. https://www.animal-reproduction.org/article/5b5a604cf7783717068b46a2

Caamaño, J., Gómez, E., Trigal, B., Muñoz, M., Carrocera, S., Martín, D., & Díez, C. (2015). Survival of vitrified in vitro-produced bovine embryos after a one-step warming instraw cryoprotectant dilution procedure. Theriogenology, 83(5), 881-890. doi: 10.1016/j.theriogenology.2014.11.021 DOI: https://doi.org/10.1016/j.theriogenology.2014.11.021

Do, V. H., Catt, S., Kinder, J. E., Walton, S., & Taylor-Robinson, A. W. (2019). Vitrification of in vitro-derived bovine embryos: targeting enhancement of quality by refining technology and standardising procedures. Reproduction, Fertility and Development, 31(5), 837-846. doi: 10.1071/RD18352 DOI: https://doi.org/10.1071/RD18352

Do, V. H., Walton, S., Catt, S., & Taylor-Robinson, A. W. (2017). A comparative analysis of the efficacy of three cryopreservation protocols on the survival of in vitro-derived cattle embryos at pronuclear and blastocyst stages. Cryobiology, 77(8), 58-63. doi: 10.1016/j.cryobiol.2017.05.007 DOI: https://doi.org/10.1016/j.cryobiol.2017.05.007

Dode, M. A. N., Leme, L., & Spricigo, J. V. W. (2013). Criopreservação de embriões bovinos produzidos in vitro. Revista Brasileira de Reprodução Animal, 37(2), 145-150. http://cbra.org.br/pages/publicacoes/rbra/v37n2/pag145-150%20(RB453).pdf

Erdem, H., Karasahin, T., Alkan, H., Dursun, S., Satilmis, F., & Guler, M. (2020). Effect of embryo quality and developmental stages on pregnancy rate during fresh embryo transfer in beef heifers. Tropical Animal Health and Production, 52(5), 2541-2547. doi: 10.1007/s11250-020-02287-6 DOI: https://doi.org/10.1007/s11250-020-02287-6

Ferré, L., Kjelland, M., Strobech, L., Hyttel, P., Mermillod, P., & Ross, P. (2020). Recent advances in bovine in vitro embryo production: reproductive biotechnology history and methods. Animals, 14(5), 991-1004. doi: 10.1017/S1751731119002775 DOI: https://doi.org/10.1017/S1751731119002775

Florentino, C., Mariani, A., Souza, J., Dias, F., Santos, H., Arrivabene, M., Neves, W., Souza, J., Cavalcante, T., & Wischral, A. (2013). Pregnancy rates bovine recipients inovulated with in vitro produced IVP embryos in the Legal Amazon. Journal of Animal Science Advances, 3(4), 193-202. doi: 10.5455/jasa.20130522044741 DOI: https://doi.org/10.5455/jasa.20130430122709

George, F., Daniaux, C., Genicot, G., Verhaeghe, B., Lambert, P., & Donnay, I. (2008). Set up of a serum-free culture system for bovine embryos: Embryo development and quality before and after transient transfer. Theriogenology, 69(5), 612-623. doi: 10.1016/j.theriogenology.2007.11.008 DOI: https://doi.org/10.1016/j.theriogenology.2007.11.008

Gómez, E., Carrocera, S., Martín, D., Pérez-janez, J. J., Prendes, J., Prendes, J. M., Vázquez, A., Murillo, A., Gimeno, I., & Muñoz, M. (2020). Efficient one-step direct transfer to recipients of thawed bovine embryos cultured in vitro and frozen in chemically defined medium. Theriogenology, 146(1), 39-47. doi: 10.1016/j.theriogenology.2020.01.056 DOI: https://doi.org/10.1016/j.theriogenology.2020.01.056

Gonçalves, R. L. R., & Viana, J. H. M. (2019). Situação atual da produção de embriões bovinos no Brasil e no mundo. Revista Brasileira de Reprodução Animal, 43(2), 156-159. http://cbra.org.br/portal/downloads/publicacoes/rbra/v43/n2/p156-159%20(RB785).pdf

Hasler, J. F., Henderson, W. B., Hurtgen, P. J., Jin, Z. Q., Mccauley, A. D., Mower, S. A., & Trimmer, S. A. (1995). Production, freezing and transfer of bovine IVF embryos and subsequent calving results. Theriogenology, 43(1), 141-152. doi: 10.1016/0093-691X(94)00020-U DOI: https://doi.org/10.1016/0093-691X(94)00020-U

Inaba, Y., Aikawa, Y., Hirai, T., Hashiyada, Y., Yamanouchi, T., Misumi, K., Ohtake, M., Somfai, T., Kobayashi, S., & Saito, N. (2011). In-straw cryoprotectant dilution for bovine embryos vitrified using Cryotop. Journal of Reproduction and Development, 11(4), 04010362. doi: 10.1262/jrd.10-154m DOI: https://doi.org/10.1262/jrd.10-154M

Leibo, S. P., & Mapletoft, R. J. (1998). Direct transfer of cryopreserved cattle embryos in North America. Proceedings of the Annual Conven Tion of American Embryo Transfer Association, San Antonio, Misiones, Argentina, 17.

Liebermann, J., & Tucker, J. (2002). Blastocyst development after vitrification of multipronuclear zygotes using the flexipet denuding pipette. Reproduction BioMedecine Online, 4(2), 146-150. doi: 10.1016/s1472-6483(10)61932-3 DOI: https://doi.org/10.1016/S1472-6483(10)61932-3

Liebermann, J., & Tucker, M. J. (2006). Comparison of vitrification and conventional cryopreservation of day 5 and day 6 blastocysts during clinical application. Fertility and Sterility, 86(1) 20-26. doi: 10.1016/j.fertnstert.2006.01.029 DOI: https://doi.org/10.1016/j.fertnstert.2006.01.029

Lonergan, P., Khatir, H., Piumi, F., Rieger, D., Humblot, P., & Boland, M. P. (1999). Effect of time interval from insemination to first cleavage on the developmental characteristics, sex ratio and pregnancy rate after transfer of bovine embryos. Journal of Reproduction and Fertility, 117(1), 159-167. doi: 10.1530/jrf.0.1170159 DOI: https://doi.org/10.1530/jrf.0.1170159

Marinho, L. S. R., Sanches, B. V., Rosa, C. O., Tannura, J. H., Rigo, A. G., Basso, A. C., & Seneda, M. M. (2015). Pregnancy rates to fixed embryo transfer of vitrified IVP Bos indicus, Bos taurus or Bos indicus × Bos taurus embryos. Reproduction in Domestic Animals, 50(5), 807-811. doi: 10.1111/rda.12591 DOI: https://doi.org/10.1111/rda.12591

Martínez-Rodero, I., García-Martínez, T., Ordóñez-Léon, E. A., Vendrell-Flotats, M., Olegario Hidalgo, C., Esmoris, J., Mendibil, X., Azcarate, S., López-Béjar, M., Yeste, M., & Mogas, T. (2021). A shorter equilibration period improves post-warming outcomes after vitrification and in straw diluition of in vitro-produced bovine embryos. Biology, 10(2), 1-15. doi: 10.3390/biology10020142 DOI: https://doi.org/10.3390/biology10020142

Morató, R., Izquierdo, D., Paramio, M. T., & Mogas, T. (2010). Survial and apoptosis rates after vitrification in cryotop devices of in vitro-produced calf and cow blastocysts ar diferente developmental stages. Reproduction, Fertility and Development, 22(7), 1141-1147. doi: 10.1071/RD10013 DOI: https://doi.org/10.1071/RD10013

Mucci, N., Aller, J., kaiser, G. G., Hozbor, F., Cabodevila, J., & Alberio, R. H. (2006). Effect of estrous cow serum during bovine embryo culture on blastocyst development and cryotolerance after slow freezing or vitrification. Theriogenology, 65(8), 1551-1562. doi: 10.1016/j.theriogenology.2005.08.020 DOI: https://doi.org/10.1016/j.theriogenology.2005.08.020

Oliveira, C. S., Feuchard, V. L. S., Freitas, C., Rosa, P. M. S., Camargo, A. J. R., & Saraiva, N. Z. (2020). In-straw warming protocol improves survival of vitrified embryos and allows direct transfer in cattle. Criobiology, 97(12), 222-225. doi: 10.1016/j.cryobiol.2020.02.007 DOI: https://doi.org/10.1016/j.cryobiol.2020.02.007

Saha, S., Rajamahendran, R., Boediono, A., Sumantril, C., & Suzuki, T. (1996). Viability of bovine blastocysts obtained after 7, 8 or 9 days of culture in vitro following vitrification and one-step rehydration. Theriogenology, 46(2), 331-343. doi: 10.1016/0093-691x(96)00189-6 DOI: https://doi.org/10.1016/0093-691X(96)00189-6

Sanches, B. V., Lunardelli, P. A., Tannura, J. H., Cardoso, B. L., Pereira, M. H. C., Gaitkoski, D., Basso, A. D., Arnold, D. R., & Seneda, M. M. (2016). A new direct transfer protocol for cryopreserved IVF embryos. Theriogenology, 85(6), 1147-1151. doi: 10.1016/j.theriogenology.2015.11.029 DOI: https://doi.org/10.1016/j.theriogenology.2015.11.029

Saragusty, J., & Arav, A. (2011). Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification. Reproduction, 141(1), 1-19. doi: 10.1530/REP-10-0236 DOI: https://doi.org/10.1530/REP-10-0236

Shu, Y., Watt, J., Gebhardt, J., Zhao, Q., Milki, A., & Behr, B. (2008). Elective single-embryo transfer in frozen blastocyst transfers cycles. Fertility and Sterility, 90(1), S205. doi: 10.1016/j.fertnstert.2008.07.484 DOI: https://doi.org/10.1016/j.fertnstert.2008.07.484

Vajta, G., Holm, P., Kuwayama, M., Booth, P. J., Jacobsen, H., Greve, T. & Callense, H. (1998). Open pulled straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Molecular Reproduction Develepment, 51(1), 51-53. doi:10.1002/(SICI)1098-2795(199809)51 1%3C53 AID-MRD6%3E3.0.CO;2-V DOI: https://doi.org/10.1002/(SICI)1098-2795(199809)51:1<53::AID-MRD6>3.0.CO;2-V

Viana, J. H. M. (2018). 2017 Statistics of embryo production and transfer in domestic farm animals. Embryo Technology Newsletter, 36(1), 8-25. https://www.iets.org/Portals/0/Documents/Public/Committees/DRC/IETS_Data_Retrieval_Report_2017.pdf

Viana, J. H. M. (2022). 2021 Statistics of embryo production and transfer in domestic farm animals. A new milestone has been reached: transfer of IVP embryos were over over one million worldwide. Embryo Transfer Newsletter, 40(4), 22-40. https://www.iets.org/Portals/0/Documents/Public/News/December_2021.pdf

Voelkel, S. A., & Hu, Y. X. (1992). Direct transfer of frozen-thawed bovine embryos. Theriogenology, 27(1), 23-30. https://doi.org/10.1016/0093-691X(92)90245-M DOI: https://doi.org/10.1016/0093-691X(92)90245-M

Zolini, A. M., Carrascal-Triana, E., King, A. R., Hansen, P. J., Torres, C. A. A., & Block, J. (2019). Effect of addition of L-carnitine to media for oocyte maturation and embryo culture on development and cryotolerance of bovine embryos produced in vitro. Theriogenology, 133(15), 135-143. doi: 10.1016/j.theriogenology.2019.05.005 DOI: https://doi.org/10.1016/j.theriogenology.2019.05.005

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Published

2024-04-17

How to Cite

Nunes, V. R. R., Gregianini, J. T. F., Gregianini, H. A. G., Fantin, B. S., Loureiro, B., & Satrapa, R. A. (2024). Comparison between vitrification and slow freezing on the post-thaw development of bovine embryos produced in vitro. Semina: Ciências Agrárias, 44(2), 537–554. https://doi.org/10.5433/1679-0359.2024v44n2p537

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