Molecular identification of bovine gammaherpesvirus 6 in water buffaloes (Bubalus bubalis) with histological evidence of pulmonary and intestinal lesions
DOI:
https://doi.org/10.5433/1679-0359.2023v44n5p1879Keywords:
BoGHV6, Bovine lymphotropic virus, Macavirus. Enteritis, Histological patterns of disease, Interstitial pneumonia.Abstract
Bovine gammaherpesvirus 6 (BoGHV6), formerly known as bovine lymphotropic virus, belongs to the genus Macavirus, which includes the porcine lymphotropic herpesviruses (PLHV) and members collectively referred to as malignant catarrhal fever virus (MVFV) group. This study investigated the possible participation of BoGHV6 in the development of intestinal and pulmonary diseases of buffaloes. Intestinal and pulmonary fragments were obtained from 19 buffaloes from Goiás, Central-western Brazil. Histopathology revealed interstitial pneumonia in all pulmonary fragments evaluated, while two of these had suppurative bronchopneumonia. Furthermore, all intestinal fragments demonstrated atrophic enteritis. BoGHV6 DNA was amplified from 31.6% (6/19) of the buffaloes with interstitial pneumonia and in 26.3% (5/19) with atrophic enteritis. The phylogenetic evaluation revealed that the strain of BoGHV6 amplified from these buffaloes had 100% nucleotide (nt) sequence identity with the reference strains of BoGHV6, but only 69.5 to 73.7% and 65.8 – 69.5% nt sequence identity with members of the MCFV and the PLHV, respectively. Additionally, molecular assays to detect important pathogens of ruminants did not amplify the respective nucleic acids in the tissues evaluated. These results demonstrated that BoGHV6 was amplified from tissues of buffaloes with histopathologic diagnoses of interstitial pneumonia and atrophic enteritis, suggesting the possible participation of this virus in the development of the intestinal and pulmonary lesions herein identified.
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Alfieri, A. A., Parazzi, M. E., Takiuchi, E., Médici, K. C., & Alfieri, A. F. (2006). Frequency of group A rotavirus in diarrhoeic calves in Brazilian cattle herds, 1998-2002. Tropical Animal Health and Production, 38(7-8), 521-526. doi: 10.1007/s11250-006-4349-9 DOI: https://doi.org/10.1007/s11250-006-4349-9
Banks, M., Ibata, G., Murphy, A. M., Frossard, J. P., Crawshaw, T. R., & Twomey, D. F. (2008). Bovine lymphotropic herpesvirus and non-responsive post-partum metritis in dairy herds in the UK. Veterinary Journal, 176(2), 248-250. doi: 10.1016/j.tvjl.2007.02.005 DOI: https://doi.org/10.1016/j.tvjl.2007.02.005
Baxter, S. I., Pow, I., Bridgen, A., & Reid, H. W. (1993). PCR detection of the sheep-associated agent of malignant catarrhal fever. Archives of Virology, 132(1-2), 145-159. doi: 10.1007/BF01309849 DOI: https://doi.org/10.1007/BF01309849
Boom, R., Sol, C. J., Salimans, M. M., Jansen, C. L., Wertheim-van Dillen, P. M., & Van der Noordaa, J. (1990). Rapid and simple method for purification of nucleic acids. Journal of Clinical Microbiology, 28(3), 495-503. doi: 10.1128/jcm.28.3.495-503.1990 DOI: https://doi.org/10.1128/jcm.28.3.495-503.1990
Claus, M. P., Alfieri, A. F., Folgueras-Flatschart, A. V., Wosiacki, S. R., Médici, K. C., & Alfieri, A. A. (2005). Rapid detection and differentiation of bovine herpesvirus 1 and 5 glycoprotein C gene in clinical specimens by multiplex-PCR. Journal of Virological Methods, 128(1-2), 183-188. doi: 10.1016/j.viromet.2005.05.001 DOI: https://doi.org/10.1016/j.jviromet.2005.05.001
Cobb, S. P., Banks, M., Russell, C., & Thorne, M. (2006). Bovine lymphotrophic herpesvirus in a UK dairy herd. Veterinary Record, 158(23), 807-808. doi: 10.1136/vr.158.23.807-a DOI: https://doi.org/10.1136/vr.158.23.807-a
Collins, J. K., Bruns, C., Vermedahl, T. L., Schiebel, A. L., Jessen, M. T., Schultheiss, P. C., Anderson, G. M., Dinsmore, R. P., Callan, R. J., & DeMartini, J. C. (2000). Malignant catarrhal fever: polymerase chain reaction survey for ovine herpesvirus 2 and other persistent herpesvirus and retrovirus infections of dairy cattle and bison. Journal of Veterinary Diagnostic Investigation, 12(5), 406-411. doi: 10.1177/104063870001200503 DOI: https://doi.org/10.1177/104063870001200503
Costa, E. A., Bastianetto, E., Vasconcelos, A. C., Bomfim, M. R. Q., Fonseca, F. G. da, Gomes, A. D., Leite, R. C., & Resende, M. (2009). An outbreak of malignant catarrhal fever in Murrah buffaloes in Minas Gerais, Brazil. Pesquisa Veterinária Brasileira, 29(5), 395-400. doi: 10.1590/S0100736X2009000500006 DOI: https://doi.org/10.1590/S0100-736X2009000500006
Crawford, T. B., Li, H., Rosenburg, S. R., Norhausen, R. W., & Garner, M. M. (2002). Mural folliculitis and alopecia caused by infection with goat-associated malignant catarrhal fever virus in two sika deer. Journal of the American Veterinary Medical Association, 221(6), 843-847. doi: 10.2460/javma.2002.221.843 DOI: https://doi.org/10.2460/javma.2002.221.843
De Boer, M. W., Zheng, T., Buddle, B. M., & McDougall, S. (2014). Detection of bovine herpesvirus type 4 antibodies and bovine lymphotropic herpesvirus in New Zealand dairy cows. New Zealand Veterinary Journal, 62(6), 351-355. doi: 10.1080/00480169.2014.933683 DOI: https://doi.org/10.1080/00480169.2014.933683
Dettwiler, M., Stahel, A., Krüger, S., Gerspach, C., Braun, U., Engels, M., & Hilbe, M. (2011). A possible case of caprine-associated malignant catarrhal fever in a domestic water buffalo (Bubalus bubalis) in Switzerland. BMC Veterinary Research, 7(78), doi: 10.1186/1746-6148-7-78 DOI: https://doi.org/10.1186/1746-6148-7-78
Gagnon, C. A., Allam, O., Drolet, R., & Tremblay, D. (2010). Quebec: detection of bovine lymphotropic herpesvirus DNA in tissues of a bovine aborted fetus. Canadian Veterinary Journal, 51(9), 1021-1022.
Garigliany, M. M., Bayrou, C., Cassart, D., Jolly, S., & Desmecht, D. (2013). Bovine lymphotropic herpesvirus detected in Belgium. Veterinary Record, 172(20), 535-536. doi: 10.1136/vr.f3127 DOI: https://doi.org/10.1136/vr.f3127
Hall, T. A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95-98.
Headley, S. A., Agnol, A. M. D., Navolar, F. M. N., Frucchi, A. P. S., Matos, A. M. R. N., Pereira, P. F. V., Xavier, A. A. C., Santos, V. H., Silva, L. E., Depes, V. C. A., Alfieri, A. F., Cunha, C. W., & Alfieri, A. A. (2021). Ovine gammaherpesvirus-2 infection associated with chronic interstitial pneumonia in a sheep. Microbial Pathogenesis, 161(PtA), 105220. doi: 10.1016/j.micpath.2021.105220 DOI: https://doi.org/10.1016/j.micpath.2021.105220
Headley, S. A., Alfieri, A. F., Oliveira, V. H. S., Beuttemmüller, E. A., & Alfieri, A. A. (2014). Histophilus somni is a potential threat to beef cattle feedlots from Brazil. Veterinary Record, 175(10), 241-249. doi: 10.1136/vr.102562 DOI: https://doi.org/10.1136/vr.102562
Headley, S. A., Dall Agnol, A. M., Bessegato, J. A., Frucchi, A. P. S., Maturana, É. F. L., Rodrigues, R. V., Xavier, A. A. C., Alfieri, A. F., & Alfieri, A. A. (2023a). Association of ovine gammaherpesvirus 2 with an outbreak of acute respiratory disease in dairy cattle. Scientific Reports, 13(1), 5623. doi: 10.1038/s41598-023-30133-w DOI: https://doi.org/10.1038/s41598-023-30133-w
Headley, S. A., Dall Agnol, A. M., Oliveira, T. E. S., Bon, V. R., Scuisato, G. S., Xavier, A. A. C., Yasumitsu, C. Y., Alfieri, A. F., & Alfieri, A. A. (2023b). Possible association of Bovine Gammaherpesvirus 6 with pulmonary disease in a cow. Animals, 13(3), 417. doi: 10.3390/ani13030417 DOI: https://doi.org/10.3390/ani13030417
Headley, S. A., Fritzen, J. T. T., Bon, V. R., Xavier, A. A. C., Dall Agnol, A. M., Zucoloto, N. Z., Silva, F. H. P., Figueiredo, J. R. X., Alfieri, A. F., Okano, W., & Alfieri, A. A. (2022b). Detection of bovine gammaherpesvirus 6 in tissues of aborted fetuses from dairy cows concomitantly infected by Histophilus somni. Microbial Pathogenesis, 169, 105621. doi: 10.1016/j.micpath.2022.105621 DOI: https://doi.org/10.1016/j.micpath.2022.105621
Headley, S. A., Lemos, G. A. A. de, Dall Agnol, A. M., Xavier, A. A. C., Depes, V. C. A., Yasumitsu, C. Y., Oliveira, T. E. S., Silva, L. E., Faccin, T. C., Alfieri, A. A., & Lisboa, J. A. N. (2022a). Ovine gammaherpesvirus 2 infections in cattle without typical manifestations of sheep-associated malignant catarrhal fever and concomitantly infected with bovine coronavirus. Brazilian Journal of Microbiology, 53(1), 433-446. doi: 10.1007/s42770-021-00653-6 DOI: https://doi.org/10.1007/s42770-021-00653-6
Headley, S. A., Müller, M. C., Oliveira, T. E. S. de, Barros Gil Duarte, C. A., Valente Pereira, P. F., Vieira, M. V., Cunha, C. W., Flores, E. F., Lisbôa, J. A. N., & Pretto-Giordano, L. G. (2020a). Diphtheric aspergillosis tracheitis with gastrointestinal dissemination secondary to viral infections in a dairy calf. Microbial Pathogenesis, 149, 104497. doi: 10.1016/j.micpath.2020.104497 DOI: https://doi.org/10.1016/j.micpath.2020.104497
Headley, S. A., Okano, W., Balbo, L. C., Marcasso, R. A., Oliveira, T. E. S., Alfieri, A. F., Negri, L. C., Fº., Michelazzo, M. M. Z., Rodrigues, S. M. C., Baptista, A. L., Saut, J. P. E., & Alfieri, A. A. (2018). Molecular survey of infectious agents associated with bovine respiratory disease in a beef cattle feedlot in southern Brazil. Journal of Veterinary Diagnostic Investigation, 30(2), 249-251. doi: 10.1177/1040638717739945 DOI: https://doi.org/10.1177/1040638717739945
Headley, S. A., Oliveira, T. E. S., & Cunha, C. W. (2020b). A review of the epidemiological, clinical, and pathological aspects of malignant catarrhal fever in Brazil. Brazilian Journal of Microbiology, 51(3), 1405-1432. doi: 10.1007/s42770-020-00273-6 DOI: https://doi.org/10.1007/s42770-020-00273-6
Headley, S. A., Pimentel, L. A., Oliveira, V. H., Toma, H. S., Alfieri, A. F., Carvalho, A. M., Santos, M. D. dos, & Alfieri, A. A. (2015). Transplacental transmission of Ovine Herpesvirus 2 in cattle with sheep-associated malignant catarrhal fever. Journal of Comparative Pathology, 153(4), 206-211. doi: 10.1016/j.jcpa.2015.10.175 DOI: https://doi.org/10.1016/j.jcpa.2015.10.175
International Committee on Taxonomy of Viruses (2022). Subfamily: Gammaherpesvirinae, Genus: Macavirus. https://ictv.global/report/chapter/herpesviridae/herpesviridae/macavirus
Kimura, M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16(2), 111-120. doi: 10. 1007/bf01731581 DOI: https://doi.org/10.1007/BF01731581
Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7), 1870-1874. doi: 10.1093/molbev/msw054 DOI: https://doi.org/10.1093/molbev/msw054
Li, H., Cunha, C. W., Taus, N. S., & Knowles, D. P. (2014). Malignant catarrhal fever: inching toward understanding. Annual Review of Animal Biosciences, 2, 209-233. doi: 10.1146/annurev-animal-022513-114156 DOI: https://doi.org/10.1146/annurev-animal-022513-114156
Li, H., Dyer, N., Keller, J., & Crawford, T. B. (2000). Newly recognized herpesvirus causing malignant catarrhal fever in white-tailed deer (Odocoileus virginianus). Journal of Clinical Microbiology, 38(4), 1313-1318. doi: 10.1128/jcm.38.4.1313-1318.2000 DOI: https://doi.org/10.1128/JCM.38.4.1313-1318.2000
Li, H., Gailbreath, K., Bender, L. C., West, K., Keller, J., & Crawford, T. B. (2003). Evidence of three new members of malignant catarrhal fever virus group in muskox (Ovibos moschatus), Nubian ibex (Capra nubiana), and gemsbok (Oryx gazella). Journal of Wildlife Diseases, 39(4), 875-880. doi: 10.7589/0090-3558-39.4.875 DOI: https://doi.org/10.7589/0090-3558-39.4.875
Li, H., Shen, D. T., Knowles, D. P., Gorham, J. R., & Crawford, T. B. (1994). Competitive inhibition enzyme-linked immunosorbent assay for antibody in sheep and other ruminants to a conserved epitope of malignant catarrhal fever virus. Journal of Clinical Microbiology, 32(7), 1674-1679. doi: 10.1128/jcm.32.7.1674-1679.1994 DOI: https://doi.org/10.1128/jcm.32.7.1674-1679.1994
Oliveira, C. H., Oliveira, F. G., Gasparini, M. R., Galinari, G. C., Lima, G. K., Fonseca, A. A., Jr., Barbosa, J. D., Barbosa-Stancioli, E. F., Leite, R. C., & Reis, J. K. dos. (2015). Bovine herpesvirus 6 in buffaloes (Bubalus bulalis) from the Amazon region, Brazil. Tropical Animal Health and Production, 47(2), 465-468. doi: 10.1007/s11250-014-0733-z DOI: https://doi.org/10.1007/s11250-014-0733-z
Oliveira, T. E. S., Scuisato, G. S., Fritzen, J. T. T., Silva, D. C., Massi, R. P., Pelaquim, I. F., Silva, L. E., Flores, E. F., Santos, R. L., Pretto-Giordano, L. G., Lisbôa, J. A. N., Alfieri, A. A., & Headley, S. A. (2022). Infectious disease agents associated with pulmonary alterations in aborted bovine fetuses.Animals, 12(13), 1596. doi: 10.3390/ani12131596 DOI: https://doi.org/10.3390/ani12131596
Oliveira, T. E. S., Scuisato, G. S., Pelaquim, I. F., Cunha, C. W., Cunha, L. S., Flores, E. F., Pretto-Giordano, L. G., Lisbôa, J. A. N., Alfieri, A. A., Saut, J. P. E., Cunha, P. H. J., & Headley, S. A. (2021). The participation of a virus within the malignant catarrhal fever virus group and Mycoplasma bovis in the development of single and mixed infections in beef and dairy cattle with bovine respiratory disease. Frontiers in Veterinary Science, 8, 691448. doi: 10.3389/fvets.2021.691448 DOI: https://doi.org/10.3389/fvets.2021.691448
Oliveira, V. H. S., Dall Agnol, A. M., Fritzen, J. T. T., Lorenzetti, E., Alfieri, A. A., & Alfieri, A. F. (2020). Microbial diversity involved in the etiology of a bovine respiratory disease outbreak in a dairy calf rearing unit. Comparative Immunology, Microbiology and Infectious Diseases, 71, 101494. doi: 10.1016/j.cimid.2020.101494 DOI: https://doi.org/10.1016/j.cimid.2020.101494
Pfitzer, S., Last, R., Espie, I., & Van Vuuren, M. (2015). Malignant catarrhal fever: an emerging disease in the African Buffalo (Syncerus caffer). Transboundary and Emerging Diseases, 62(3), 288-294. doi: 10.1111/tbed.12131 DOI: https://doi.org/10.1111/tbed.12131
Rosato, G., Ruiz Subira, A., Al-Saadi, M., Michalopoulou, E., Verin, R., Dettwiler, M., Nordgren, H., Chiers, K., Groβmann, E., Köhler, K., Suntz, M., Stewart, J. P., & Kipar, A. (2021). Gammaherpesvirus infections in cattle in Europe. Viruses, 13(12), 2337. doi: 10.3390/v13122337 DOI: https://doi.org/10.3390/v13122337
Rovnak, J., Quackenbush, S. L., Reyes, R. A., Baines, J. D., Parrish, C. R., & Casey, J. W. (1998). Detection of a novel bovine lymphotropic herpesvirus. Journal of Virology, 72(5), 4237-4242. doi: 10.1128/jvi.72.5.4237-4242.1998 DOI: https://doi.org/10.1128/JVI.72.5.4237-4242.1998
Russell, G. C., Stewart, J. P., & Haig, D. M. (2009). Malignant catarrhal fever: a review. Veterinary Journal, 179(3), 324-335. doi: 10.1016/j.tvjl.2007.11.007 DOI: https://doi.org/10.1016/j.tvjl.2007.11.007
Stahel, A. B., Baggenstos, R., Engels, M., Friess, M., & Ackermann, M. (2013). Two different macaviruses, ovine herpesvirus-2 and caprine herpesvirus-2, behave differently in water buffaloes than in cattle or in their respective reservoir species. PLoS One, 8(12), e83695. doi: 10.1371/journal.pone.0083695 DOI: https://doi.org/10.1371/journal.pone.0083695
Takiuchi, E., Stipp, D. T., Alfieri, A. F., & Alfieri, A. A. (2006). Improved detection of bovine coronavirus N gene in faeces of calves infected naturally by a semi-nested PCR assay and an internal control. Journal of Virological Methods, 131(2), 148-154. doi: 10.1016/j.jviromet.2005.08.005 DOI: https://doi.org/10.1016/j.jviromet.2005.08.005
Tham, K. M. (1997). Molecular and clinicopathological diagnosis of malignant catarrhal fever in cattle, deer and buffalo in New Zealand. Veterinary Record, 141(12), 303-306. doi: 10.1136/vr.141.12.303 DOI: https://doi.org/10.1136/vr.141.12.303
Van der Maaten, M. J., & Boothe, A. D. (1972). Isolation of a herpes-like virus from lymphosarcomatous cattle. Archiv fur die Gesamte Virusforschung, 37(1), 85-96. doi: 10.1007/bf01241154 DOI: https://doi.org/10.1007/BF01241154
Vilcek, S., Elvander, M., Ballagi-Pordany, A., & Belak, S. (1994a). Development of nested PCR assays for detection of bovine respiratory syncytial virus in clinical samples. Journal of Clinical Microbiology, 32(9), 2225-2231. doi: 10.1128/jcm.32.9.2225-2231.1994. DOI: https://doi.org/10.1128/jcm.32.9.2225-2231.1994
Vilcek, S., Herring, A. J., Herring, J. A., Nettleton, P. F., Lowings, J. P., & Paton, D. J. (1994b). Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Archives of Virology, 136(3-4), 309-323. doi: 10.1007/bf01321060 DOI: https://doi.org/10.1007/BF01321060
Zhu, Y. M., Shi, H. F., Gao, Y. R., Xin, J. Q., Liu, N. H., Xiang, W. H., Ren, X. G., Feng, J. K., Zhao, L. P., & Xue, F. (2011). Isolation and genetic characterization of bovine parainfluenza virus type 3 from cattle in China. Veterinary Microbiology, 149(3-4), 446-451. doi: 10.1016/j.vetmic.2010.11.011 DOI: https://doi.org/10.1016/j.vetmic.2010.11.011
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