Immunoassays: an alternative for toxigenic fungi detection in foods
DOI:
https://doi.org/10.5433/1679-0359.2006v27n4p613Keywords:
Immunoassays, Toxigenic fungi, Mould detection, Immunodetection.Abstract
Constant monitoring of mould contamination is essential in order to assure the food quality and safety and reduce the economic losses, as well as to minimize the potential hazards to human and animal health. The traditional methods for mould identification and detection (culture in several media, microscopic examination and chemical analysis) are usually time-consuming and require trained staff. Immunoassays, particularly enzyme-linked immunosorbent assay (ELISA) could be a promising alternative to the traditional methods due to high sensitivity, specificity, reproducibility and potential for use in rapid quality control. Among ELISAs, those based on exoantigens are the most employed in the resolution of taxonomic problems, detection and identification of toxigenic fungi. This review discusses the basic principles of immunoassays, methods of mould detection and the several ELISAs developed for toxigenic fungi detection in food.
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