Comparative study of two serological tests for detection of anti-Theileria equi antibodies in horses
DOI:
https://doi.org/10.5433/1679-0359.2015v36n6Supl2p4361Keywords:
Piroplasmosis, Equids, Seroepidemiology, Theileriosis.Abstract
Theileria equi is the causative agent of worldwide piroplasmosis, an important tick-borne disease of equids associated to a lifetime carrier state of infected horses. Since rapid, accessible and reliable tests have been posted as a challenge for clinicians, the aim of the present study was to verify the agreement between an in-house immunofluorescence antibody assay (IFA) and a commercial competitive inhibition enzyme-linked immunosorbent assay (cELISA) in a population of 198 carthorses from Southern Brazil. The horse seroreactivity for T. equi revealed 152/198 (76.8%) positive samples by IFA and 155/198 (78.3%) by cELISA. Using cELISA as gold standard, IFA has shown a sensitivity of 91.6% (95% CI = 86.18-95.03%) and specificity of 76.7% (95% CI = 62.26-86.85%), with a substantial degree of agreement (k = 0.8445). In conclusion, the in-house IFA may be used as a screening test for diagnosis of equine piroplasmosis.
Downloads
Published
How to Cite
Issue
Section
License
Semina: Ciências Agrárias adopts the CC-BY-NC license for its publications, the copyright being held by the author, in cases of republication we recommend that authors indicate first publication in this journal.
This license allows you to copy and redistribute the material in any medium or format, remix, transform and develop the material, as long as it is not for commercial purposes. And due credit must be given to the creator.
The opinions expressed by the authors of the articles are their sole responsibility.
The magazine reserves the right to make normative, orthographic and grammatical changes to the originals in order to maintain the cultured standard of the language and the credibility of the vehicle. However, it will respect the writing style of the authors. Changes, corrections or suggestions of a conceptual nature will be sent to the authors when necessary.