Survival and development in vitro of Cattleya (Orchidaceae) submitted to treatments with different treatments of colchicines

Authors

  • Alessandro Borini Lone Universidade Estadual de Londrina
  • Lílian Keiko Unemoto Universidade Estadual de Londrina
  • Lúcia Sadayo Assari Takahashi Universidade Estadual de Londrina
  • Ricardo Tadeu Faria Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/1679-0359.2010v31n4Sup1p1337

Keywords:

Cattleya tigrina, Polyploidy, Protocorms

Abstract

Polyploidy produces desirable characteristicsin orchids that are represented by increase of the floral pieces, succulency degree, intensification of color, durability and larger resistance of  flowers. The colchicine as polyploidy inductor agent in in vitro culture of plants has limitations, because when in high concentrations or very long treatments, it becomes poisonous for the vegetal tissues. The objective of this research work was to evaluate the influence of concentrations and times of exposition to the colchicine, on the survival and on the in vitro development of Cattleya tigrina. Protocorms were treated with colchicine in the concentrations of 0.5 and 1g.L-1 during 24, 48 and 72 hours. There were evaluated the tax of survival of the protocorms (%), percentage of plantlings with multiple buds and plant height after three and seven months. The increase of the concentration and of the time of exposition to  colchicine caused a larger plant mortality and an increase of  plantlings with reduced height. 

Author Biographies

Alessandro Borini Lone, Universidade Estadual de Londrina

Biólogo, Mestrando em Fitotecnia no Departamento de Agronomia da Universidade Estadual de Londrina.

Lílian Keiko Unemoto, Universidade Estadual de Londrina

Bióloga, Doutora em Fitotecnia pela Universidade Estadual de Londrina/UEL, Londrina, PR.

Lúcia Sadayo Assari Takahashi, Universidade Estadual de Londrina

Engenheira Agrônoma, Professora, Dra. do Departamento de Agronomia da Universidade Estadual de Londrina,/UEL, Londrina, PR.

Ricardo Tadeu Faria, Universidade Estadual de Londrina

Engenheiro Agrônomo, Professor, Dr. do Departamento de Agronomia da Universidade Estadual de Londrina/UEL, Londrina, PR.

Published

2011-02-09

How to Cite

Lone, A. B., Unemoto, L. K., Takahashi, L. S. A., & Faria, R. T. (2011). Survival and development in vitro of Cattleya (Orchidaceae) submitted to treatments with different treatments of colchicines. Semina: Ciências Agrárias, 31(4Sup1), 1337–1342. https://doi.org/10.5433/1679-0359.2010v31n4Sup1p1337

Issue

Section

Communication

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