Tissue explants: a model rediscovered in animal experiments

Abstract

The ex vivo technique was developed in the 30's and in recent years, due to ethical and legal issues in laboratory experiments, was rediscovered as effective technique. Pigs are often used as animal model in this technique due to the high similarity in various organ systems with humans. Explants of intestine, skin, joint, lung and bronchus of pigs have been used in animal experiments, with different purposes, such as evaluating the effects of toxic, carcinogenic, therapeutic, and biological agents or sensitivity tests. The use of explants minimizes ethical problems and lack of human samples, that often affect researches. A major limitation of the technique is time cell viability, due to hypoxia that occurs during incubation. Moreover, the method allows more controlled experimental procedures associated with a reduction in the number of animals, expanding the areas of scientific research.