Inactivation kinetics of peroxidase from coconut water

Abstract

The aim of this project was study the thermal inactivation kinetics of the peroxidase enzyme (POD) present in the green coconut water, being determined the parameters D values, z value and Q₁₀, besides the Arrhenius' activation energy (E_a) to the process. Nine time/temperature binomials for the thermal treatment at three temperatures (75°C, 85°C and 95°C) and maintenance times from 1min to 30 min, besides a control sample without thermal treatment. D values were obtained by linear regression, being equals to 833,3 min, 35,6min and 1,63min at 75°C, 85°C and 95°C, respectively, while the z value was equal to 7,4°C. These values demonstrate that POD of coconut water is thermal resistant to inactivation, being such processes strongly affected by temperature. Such fact is also verified by the high values of E_a (332,3 kJ/mol) and Q₁₀ (22,7), which indicate that a high amount of energy is required for the POD inactivation and an increase of temperature elevate the inactivation rate.