Sacarificação do caule do abacaxizeiro e sua fermentação por Saccharomyces cerevisiae

Abstract

The preliminary results obtained in the study on the production of enzymatic hydrolysate from pineapple plant stem and its fermentation to ethanol by the yeast Saccharomyces cerevisiae are presented. The dried and milled (40 mesh screen) plant stem was pretreated at 100°C for 10 min. in 1% aqueous solution before its saccharification with amiloglucosidase (50 IU/mg). Starch sacchariflcation yield was 71.77%. The alcoholic fermentation was performed on the hydrolysate (reducing sugar content: 3.3% d.w.) with the addition of 1% yeast extract, 1% ammonium sulphate. The yeast Saccharomyces cerevisiae was able to transform the substrate to ethanol at 30° pH 3.5 and 50 cpm of agitation. For each gram of reduced sugar, 0.431g of ethanol and 0.0447 g of biomass were produced. The alcoholic yield was 84.46% of the theoretical value. In view of these results, it is concluded that the pineapple plant stem could yield 282 Liters of ethanol/ton.