Antioxidant and physiological responses of seeds of soybean cultivars to delayed harvest
DOI:
https://doi.org/10.5433/1679-0359.2022v43n3p1127Keywords:
Glycine max (L.) Merrill, Delayed harvest, Enzymatic activity.Abstract
Soybean seeds are susceptible to field deterioration after reaching physiological maturity, which usually occurs at the R7 stage, with harvest at R8. This study aimed to evaluate the biochemical and physiological changes in seeds of different soybean cultivars submitted to delayed harvest in the field. Seeds of the cultivars NS 5959, BMX Potência, and TMG 1175 were harvested at four times (0, 10, 20, and 30 days after reaching R8). Moisture, germination, first count, accelerated aging, emergence, and tetrazolium tests were performed in each harvest period. The activities of the antioxidant enzymes catalase (CAT), peroxidase (POX), and ascorbate peroxidase (APX) were also evaluated. The experiment was carried out in a completely randomized design in a 3×4 factorial scheme (three cultivars and four harvest times). A reduction in germination and vigor of seeds of different soybean cultivars was found at R8 + 20 and R8 + 30 days related to field deterioration due to delayed harvest. The seeds of the cultivars did not differ in terms of viability under delayed harvest but differed in terms of antioxidant and physiological responses. Better and worse seed performances were found for the cultivars BMX Potência and TMG 1175, respectively, at R8 and in R8 + 10 days.Downloads
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Castro, E. M., Oliveira, J. A., Lima, A. E., Santos, H. O., & Barbosa, J. I. L. (2016). Physiological quality of soybean seeds produced under artificial rain in the pre-harvesting period. Journal of Seed Science, 38(1), 14-21. doi: 10.1590/2317-1545v38n1154236
Companhia Nacional de Abastecimento (2020). Safra brasileira de grãos. Recuperado de https://www.conab. gov.br/info-agro/safras/graos
Diniz, F. O., Reis, M. S., Dias, L. A. S., Araújo, E. F., Sediyama, T., & Sediyama, C. A. (2013). Physiological quality of soybean seeds of cultivars submitted to harvesting delay and its association with seedling emergence in the field. Journal of Seed Science, 35(2), 147-152. doi: 10.1590/S2317-15372013000200 002
Ebone, L. A., Caverzan, A., & Chavarria, G. (2019). Physiologic alterations in orthodox seeds due to deterioration processes. Plant Physiology and Biochemistry, 145(2019), 34-42. doi: 10.1016/j.plaphy. 2019.10.028
Fehr, W. R., & Caviness, C. E. (1977). Stages of soybean development. Special Report, 80, 1-12. doi: lib.dr. iastate.edu/specialreports/87/
Forti, V. A., Carvalho, C., Tanaka, F. A. O., & Cicero, S. M. (2013). Weathering damage in soybean seeds: assessment, seed anatomy and seed physiological potential. Seed Technology, 35(2), 213-224. doi: www. jstor.org/stable/24642271
França, J. B., Neto, & Krzyzanowski, F. C. (2020). Teste de tetrazólio em sementes de soja. In F. C. Krzyzanowski, R. D. Vieira, J. Marcos-Filho, & J. B. França-Neto (Eds.), Vigor de sementes: conceitos e testes (pp. 519-580). Londrina: Abrates.
França, J. B., Neto, Krzyzanowski, F. C., Henning, A. A., Pádua, G. P., Lorini, I., & Henning, F. A. (2016). Tecnologia da produção de semente de soja de alta qualidade. Londrina: Abrates.
Huth, C., Mertz-Henning, L. M., Lopes, S. J., Tabaldi, L. A., Rossato, L. V., Krzyzanowski, F. C., & Henning, F. A. (2016). Susceptibility to weathering damage and oxidative stress on soybean seeds with different lignin contents in the seed coat. Journal of Seed Science, 38(4), 296-304. doi: 10.1590/2317-1545v38 n4162115
Kar, M., & Mishra, D. (1976). Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant Physiology, 57(2), 315-319. doi: 10.1104/pp.57.2.315
Krzyzanowski, F. C., França, J. B., Neto, & Henning, A. A. (2018). A alta qualidade da semente de soja: fator importante para a produção da cultura. Londrina: EMBRAPA. Recuperado de https://www.embrapa.br/ busca-de-publicacoes/-/publicacao/1091765/a-alta-qualidade-da-semente-de-soja-fator-importante-para-a-producao-da-cultura
Krzyzanowski, F. C., França, J. B., Neto, Gomes, F. G., Jr., & Nakagawa, J. (2020). Testes de vigor baseados em desempenho de plântulas. In F. C. Krzyzanowski, R. D. Vieira, J. Marcos-Filho, & J. B. França-Neto (Eds.), Vigor de sementes: conceitos e testes (pp. 79-140). Londrina: Abrates.
Kurek, K., Plitta-Michalak, B., & Ratajczak, E. (2019). Reactive oxygen species as potential drivers of the seed aging process. Plants, 8(6), 174. doi: 2223-7747/8/6/174
Lima, W. A. A., Borém, A., Dias, D. C. F. S., Moreira, M. A., Dias, L. A. S., & Piovesan, N. D. (2007). Retardamento de colheita como método de diferenciação de genótipos de soja para qualidade de sementes. Revista Brasileira de Sementes, 29(1), 186-192. doi: 10.1590/S0101-31222007000100026
Marcos-Filho, J. (2020). Teste de envelhecimento acelerado. In F. C. Krzyzanowski, R. D. Vieira, J. Marcos-Filho, & J. B. França-Neto (Eds.), Vigor de sementes: conceitos e testes (pp. 185-246). Londrina: Abrates.
Mathias, V., Pereira, T., Mantovani, A., Zílio, M., Miotto, P., & Coelho, C. M. M. (2017). Implicações da época de colheita sobre a qualidade fisiológica de sementes de soja. Revista Agro@mbiente On-Line, 11(3), 223-231. doi: 10.18227/1982-8470ragro.v11i3.3894
Min, C. W., Lee, S. H., Cheon, Y. E., Han, W. Y., Ko, J .M., Kang, H. W.,… Kim, S. T. (2017). In-depth proteomic analysis of Glycine max seeds during controlled deterioration treatment reveals a shift in seed metabolism. Journal of Proteomics, 169(2017), 125-135. doi: S1874391917302324
Ministério da Agricultura, Pecuária e Abastecimento (2009). Regras para análise de sementes. Brasília: MAPA/ACS. Recuperado de http://www.agricultura.gov.br/assuntos/insumos-agropecuarios/arquivos-publicacoes-insumos/2946_regras_analise__sementes.pdf
Ministério da Agricultura, Pecuária e Abastecimento (2013). Padrões para produção e comercialização de sementes de soja (Glycine max (L.) Merrill). Instrução Normativa no 45. Brasília: MAPA/ACS. Recuperado de https://www.gov.br/agricultura/pt-br/assuntos/insumos-agropecuarios/insumos-agricolas/sementes-e-mudas/publicacoes-sementes-e mudas/copy_of_INN45de17desetembrode2013.pdf
Nakano, Y., & Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22(5), 867-880. doi: 10.1093/oxfordjournals.pcp.a076232
Peixoto, P. H. P., Cambraia, J., Sant’Anna, R., Mosquim, P. R., & Moreira, M. A. (1999). Aluminum effects on lipid peroxidation and on the activities of enzymes of oxidative metabolism in sorghum. Revista Brasileira de Fisiologia Vegetal, 11(3), 137-143.
Pinheiro, D. T., Dias, D. C. F. S., Medeiros, A. D., Ribeiro, J. P. O., Silva, F. L., & Silva, L. J. (2021). Weathering deterioration in pre-harvest of soybean seeds: physiological, physical, and morpho-anatomical changes. Scientia Agricola, 78(Suppl), e20200166. doi: 10.1590/1678-992X-2020-0166
R Core Team (2020). R: a language and environment for statistical computing. Vienna: R Foundation for Statistical Computing. Retrieved from http://www.R-project.org/
Rajjou, L., & Debeaujon, I. (2008). Seed longevity: survival and maintenance of high germination ability of dry seeds. Comptes Rendus Biologies, 331(10), 796-805. doi: S1631069108002011
Sediyama, T., Silva, F., & Borém, A. (2015). Soja: do plantio à colheita. Viçosa, MG: Universidade Federal de Viçosa.
Sharma, P., Jha, A. B., Dubey, R. S., & Pessarakli, M. (2012). Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany, 2012(1), 1-26. doi: 2012/217037/
Shu, Y., Zhou, Y., Mu, K., Hu, H., Chen, M., He, Q.,… Yu, X. A. (2020). A transcriptomic analysis reveals soybean seed pre-harvest deterioration resistance pathways under high temperature and humidity stress. Genome, 63(2), 115-124. doi: 10.1139/gen-2019-0094
Usha, T. N., & Dadlani, M. (2016). Study of free radical and peroxide scavenging enzymes and content in different vigour lots of soybean (Glycine max). Legume Research - An International Journal, 39(2), 233-236. doi: 10.1590/0103-8478cr20141736
Vergara, R., Silva, R. N. O., Nadal, A. P., Gadotti, G. I., Aumonde, T. Z., & Villela, F. A. (2019). Harvest delay, storage and physiological quality of soybean seeds. Journal of Seed Science, 41(4), 506-513. doi: 10.1590/2317-1545v41n4222413
Vieira, B., Barbosa, R. M., Helena, S., Trevisoli, U., Orlando, A., & Vieira, R. D. (2013). Biochemical alterations in soybean seeds with harvesting time and storage temperature. Journal of Food, Agriculture & Environment, 11(3&4), 887-891.
Wang, L., Ma, H., Song, L., Shu, Y., & Gu, W. (2012). Comparative proteomics analysis reveals the mechanism of pre-harvest seed deterioration of soybean under high temperature and humidity stress. Journal of Proteomics, 75(7), 2109-2127. doi: 10.1016/j.jprot.2012.01.007
Xavier, T. S., Daronch, D. J., Peluzio, J. M., Afférri, F. S., Carvalho, E. V., & Santos, W. F. (2015). Época de colheita na qualidade de sementes de genótipos de soja. Comunicata Scientiae, 6(2), 241-245. doi: 10. 14295/cs.v6i2.752
Zanatta, T. P., Kulczynski, S. M., Libera, D. D., Testa, V., Fontana, D., Werner, C.,... Michellotti, E. (2018). Produtividade e qualidade fisiológica de sementes de soja colhidas em diferentes períodos de maturação. Revista Cultivando o Saber, 11(1), 92-109.
Zhang, K., Zhang, Y., Sun, J., Meng, J., & Tao, J. (2021). Deterioration of orthodox seeds during ageing: Influencing factors, physiological alterations and the role of reactive oxygen species. Plant Physiology and Biochemistry, 158(2021), 475-485. doi: 10.1016/j.plaphy.2020.11.031
Zuffo, A. M., Zuffo, J. M., Jr., Carvalho, E. R., Steiner, F., & Zambiazzi, E. V. (2017). Physiological and enzymatic changes in soybean seeds submitted to harvest delay. Pesquisa Agropecuária Tropical, 47(4), 488-496. doi: 10.1590/1983-40632017v4749811
Bailly, C., Leymarie, J., Lehner, A., Rousseau, S., Côme, D., & Corbineau, F. (2004). Catalase activity and expression in developing sunflower seeds as related to drying. Journal of Experimental Botany, 55(396), 475-483. doi: 10.1093/jxb/erh050
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2), 248-254. doi: 10.1016/0 003-2697(76)90527-3
Castro, E. M., Oliveira, J. A., Lima, A. E., Santos, H. O., & Barbosa, J. I. L. (2016). Physiological quality of soybean seeds produced under artificial rain in the pre-harvesting period. Journal of Seed Science, 38(1), 14-21. doi: 10.1590/2317-1545v38n1154236
Companhia Nacional de Abastecimento (2020). Safra brasileira de grãos. Recuperado de https://www.conab. gov.br/info-agro/safras/graos
Diniz, F. O., Reis, M. S., Dias, L. A. S., Araújo, E. F., Sediyama, T., & Sediyama, C. A. (2013). Physiological quality of soybean seeds of cultivars submitted to harvesting delay and its association with seedling emergence in the field. Journal of Seed Science, 35(2), 147-152. doi: 10.1590/S2317-15372013000200 002
Ebone, L. A., Caverzan, A., & Chavarria, G. (2019). Physiologic alterations in orthodox seeds due to deterioration processes. Plant Physiology and Biochemistry, 145(2019), 34-42. doi: 10.1016/j.plaphy. 2019.10.028
Fehr, W. R., & Caviness, C. E. (1977). Stages of soybean development. Special Report, 80, 1-12. doi: lib.dr. iastate.edu/specialreports/87/
Forti, V. A., Carvalho, C., Tanaka, F. A. O., & Cicero, S. M. (2013). Weathering damage in soybean seeds: assessment, seed anatomy and seed physiological potential. Seed Technology, 35(2), 213-224. doi: www. jstor.org/stable/24642271
França, J. B., Neto, & Krzyzanowski, F. C. (2020). Teste de tetrazólio em sementes de soja. In F. C. Krzyzanowski, R. D. Vieira, J. Marcos-Filho, & J. B. França-Neto (Eds.), Vigor de sementes: conceitos e testes (pp. 519-580). Londrina: Abrates.
França, J. B., Neto, Krzyzanowski, F. C., Henning, A. A., Pádua, G. P., Lorini, I., & Henning, F. A. (2016). Tecnologia da produção de semente de soja de alta qualidade. Londrina: Abrates.
Huth, C., Mertz-Henning, L. M., Lopes, S. J., Tabaldi, L. A., Rossato, L. V., Krzyzanowski, F. C., & Henning, F. A. (2016). Susceptibility to weathering damage and oxidative stress on soybean seeds with different lignin contents in the seed coat. Journal of Seed Science, 38(4), 296-304. doi: 10.1590/2317-1545v38 n4162115
Kar, M., & Mishra, D. (1976). Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant Physiology, 57(2), 315-319. doi: 10.1104/pp.57.2.315
Krzyzanowski, F. C., França, J. B., Neto, & Henning, A. A. (2018). A alta qualidade da semente de soja: fator importante para a produção da cultura. Londrina: EMBRAPA. Recuperado de https://www.embrapa.br/ busca-de-publicacoes/-/publicacao/1091765/a-alta-qualidade-da-semente-de-soja-fator-importante-para-a-producao-da-cultura
Krzyzanowski, F. C., França, J. B., Neto, Gomes, F. G., Jr., & Nakagawa, J. (2020). Testes de vigor baseados em desempenho de plântulas. In F. C. Krzyzanowski, R. D. Vieira, J. Marcos-Filho, & J. B. França-Neto (Eds.), Vigor de sementes: conceitos e testes (pp. 79-140). Londrina: Abrates.
Kurek, K., Plitta-Michalak, B., & Ratajczak, E. (2019). Reactive oxygen species as potential drivers of the seed aging process. Plants, 8(6), 174. doi: 2223-7747/8/6/174
Lima, W. A. A., Borém, A., Dias, D. C. F. S., Moreira, M. A., Dias, L. A. S., & Piovesan, N. D. (2007). Retardamento de colheita como método de diferenciação de genótipos de soja para qualidade de sementes. Revista Brasileira de Sementes, 29(1), 186-192. doi: 10.1590/S0101-31222007000100026
Marcos-Filho, J. (2020). Teste de envelhecimento acelerado. In F. C. Krzyzanowski, R. D. Vieira, J. Marcos-Filho, & J. B. França-Neto (Eds.), Vigor de sementes: conceitos e testes (pp. 185-246). Londrina: Abrates.
Mathias, V., Pereira, T., Mantovani, A., Zílio, M., Miotto, P., & Coelho, C. M. M. (2017). Implicações da época de colheita sobre a qualidade fisiológica de sementes de soja. Revista Agro@mbiente On-Line, 11(3), 223-231. doi: 10.18227/1982-8470ragro.v11i3.3894
Min, C. W., Lee, S. H., Cheon, Y. E., Han, W. Y., Ko, J .M., Kang, H. W.,… Kim, S. T. (2017). In-depth proteomic analysis of Glycine max seeds during controlled deterioration treatment reveals a shift in seed metabolism. Journal of Proteomics, 169(2017), 125-135. doi: S1874391917302324
Ministério da Agricultura, Pecuária e Abastecimento (2009). Regras para análise de sementes. Brasília: MAPA/ACS. Recuperado de http://www.agricultura.gov.br/assuntos/insumos-agropecuarios/arquivos-publicacoes-insumos/2946_regras_analise__sementes.pdf
Ministério da Agricultura, Pecuária e Abastecimento (2013). Padrões para produção e comercialização de sementes de soja (Glycine max (L.) Merrill). Instrução Normativa no 45. Brasília: MAPA/ACS. Recuperado de https://www.gov.br/agricultura/pt-br/assuntos/insumos-agropecuarios/insumos-agricolas/sementes-e-mudas/publicacoes-sementes-e mudas/copy_of_INN45de17desetembrode2013.pdf
Nakano, Y., & Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22(5), 867-880. doi: 10.1093/oxfordjournals.pcp.a076232
Peixoto, P. H. P., Cambraia, J., Sant’Anna, R., Mosquim, P. R., & Moreira, M. A. (1999). Aluminum effects on lipid peroxidation and on the activities of enzymes of oxidative metabolism in sorghum. Revista Brasileira de Fisiologia Vegetal, 11(3), 137-143.
Pinheiro, D. T., Dias, D. C. F. S., Medeiros, A. D., Ribeiro, J. P. O., Silva, F. L., & Silva, L. J. (2021). Weathering deterioration in pre-harvest of soybean seeds: physiological, physical, and morpho-anatomical changes. Scientia Agricola, 78(Suppl), e20200166. doi: 10.1590/1678-992X-2020-0166
R Core Team (2020). R: a language and environment for statistical computing. Vienna: R Foundation for Statistical Computing. Retrieved from http://www.R-project.org/
Rajjou, L., & Debeaujon, I. (2008). Seed longevity: survival and maintenance of high germination ability of dry seeds. Comptes Rendus Biologies, 331(10), 796-805. doi: S1631069108002011
Sediyama, T., Silva, F., & Borém, A. (2015). Soja: do plantio à colheita. Viçosa, MG: Universidade Federal de Viçosa.
Sharma, P., Jha, A. B., Dubey, R. S., & Pessarakli, M. (2012). Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany, 2012(1), 1-26. doi: 2012/217037/
Shu, Y., Zhou, Y., Mu, K., Hu, H., Chen, M., He, Q.,… Yu, X. A. (2020). A transcriptomic analysis reveals soybean seed pre-harvest deterioration resistance pathways under high temperature and humidity stress. Genome, 63(2), 115-124. doi: 10.1139/gen-2019-0094
Usha, T. N., & Dadlani, M. (2016). Study of free radical and peroxide scavenging enzymes and content in different vigour lots of soybean (Glycine max). Legume Research - An International Journal, 39(2), 233-236. doi: 10.1590/0103-8478cr20141736
Vergara, R., Silva, R. N. O., Nadal, A. P., Gadotti, G. I., Aumonde, T. Z., & Villela, F. A. (2019). Harvest delay, storage and physiological quality of soybean seeds. Journal of Seed Science, 41(4), 506-513. doi: 10.1590/2317-1545v41n4222413
Vieira, B., Barbosa, R. M., Helena, S., Trevisoli, U., Orlando, A., & Vieira, R. D. (2013). Biochemical alterations in soybean seeds with harvesting time and storage temperature. Journal of Food, Agriculture & Environment, 11(3&4), 887-891.
Wang, L., Ma, H., Song, L., Shu, Y., & Gu, W. (2012). Comparative proteomics analysis reveals the mechanism of pre-harvest seed deterioration of soybean under high temperature and humidity stress. Journal of Proteomics, 75(7), 2109-2127. doi: 10.1016/j.jprot.2012.01.007
Xavier, T. S., Daronch, D. J., Peluzio, J. M., Afférri, F. S., Carvalho, E. V., & Santos, W. F. (2015). Época de colheita na qualidade de sementes de genótipos de soja. Comunicata Scientiae, 6(2), 241-245. doi: 10. 14295/cs.v6i2.752
Zanatta, T. P., Kulczynski, S. M., Libera, D. D., Testa, V., Fontana, D., Werner, C.,... Michellotti, E. (2018). Produtividade e qualidade fisiológica de sementes de soja colhidas em diferentes períodos de maturação. Revista Cultivando o Saber, 11(1), 92-109.
Zhang, K., Zhang, Y., Sun, J., Meng, J., & Tao, J. (2021). Deterioration of orthodox seeds during ageing: Influencing factors, physiological alterations and the role of reactive oxygen species. Plant Physiology and Biochemistry, 158(2021), 475-485. doi: 10.1016/j.plaphy.2020.11.031
Zuffo, A. M., Zuffo, J. M., Jr., Carvalho, E. R., Steiner, F., & Zambiazzi, E. V. (2017). Physiological and enzymatic changes in soybean seeds submitted to harvest delay. Pesquisa Agropecuária Tropical, 47(4), 488-496. doi: 10.1590/1983-40632017v4749811
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2022-03-16
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Fialho, C. A., Dias, D. C. F. dos S., Pinheiro, D. T., Oliveira, A. M. S., Soares, T. F. S. N., & Silva, L. J. da. (2022). Antioxidant and physiological responses of seeds of soybean cultivars to delayed harvest. Semina: Ciências Agrárias, 43(3), 1127–1144. https://doi.org/10.5433/1679-0359.2022v43n3p1127
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