Effect of adding anethole to the cryopreservation medium (powdered coconut water) on morphology, kinetics, and oxidative stress of buck sperm
DOI:
https://doi.org/10.5433/1679-0359.2021v42n6p3235Keywords:
Cryopreservation, Oxidative stress, Reproduction, Semen.Abstract
The quality of post-thawing goat sperm is critical to the success of artificial insemination protocols and may be influenced by extenders, cryoprotectants, and antioxidant substances. Therefore, the objective of this study was to evaluate the effects of the antioxidant anethole on goat sperm diluted in preservation medium based on powdered coconut water (ACP-101c) and frozen. For that, each ejaculate was submitted to the following treatments: ACP-101c (control); control plus supplementation with 30, 300, or 2000 µg/mL anethole. The samples were thawed and evaluated for morphology, kinetics, membrane integrity, and reactive oxygen species (ROS). The addition of anethole increased morphological abnormalities (P < 0.05), however, it did not affect sperm kinetics. Flow cytometry analysis showed that sperm cells cryopreserved with 300 µg/mL anethole had lower acrosome integrity than those cryopreserved in other treatments. Evaluation of oxidative stress revealed that cells stored in the presence of 2000 µg/mL anethole had small amounts of ROS when compared to those preserved in the control medium alone or supplemented with 300 µg/mL anethole (P < 0.05). After cryopreservation of sperm with 2000 µg/mL anethole, the highest percentage of viable sperm without ROS was observed (P < 0.05). In conclusion, despite reducing ROS levels, the supplementation of anethole in ACP-101c did not affect sperm kinetics or membrane integrity post-thawing, however, it did cause morphological damage to sperm.Downloads
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References
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Câmara, T. S., Sousa, A., Jr., Barçante, F. P. S., Silva, J. H. L., Sousa, M. S., Machado, A. A. C.,... Nunes, J. F. (2019). Comparação da qualidade seminal de caprinos das raças Canindé e Alpina Britânica no Nordeste brasileiro. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 71(04), 1260-1268. doi: 10.1590/1678-4162-10297
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Chainy, G. B. N., Manna, S. K., Chaturvedi, M. M., & Aggarwal, B. B. (2000). Anethole blocks both early and late cellular responses transduced by tumor necrosis factor: Effect on NF-κB, AP-1, JNK, MAPKK and apoptosis. Oncogene, 19(25), 2943-2950. doi: 10.1038/sj.onc.1203614
Chelucci, S., Pasciu, V., Succu, S., Addis, D., Leoni, G. G., Manca, M. E., & Berlinguer, F. (2015). Soybean lecithin-based extender preserves spermatozoa membrane integrity and fertilizing potential during goat semen cryopreservation. Theriogenology, 83(6), 1064-1074. doi: 10.1016/j.theriogenology.2014.12.012
Choi, E., & Hwang, J. (2004) Antiinflammatory, analgesic and antioxidant activities of the fruit of Foeniculum vulgare. Fitoterapia, 75(6), 557-565. doi: 10.1016/j.fitote.2004.05.005
Dringen, R., Hamprecht, B., & Drukarch, B. (1998). Anethole dithiolethione, a putative neuroprotectant, increases intracellular and extracellular glutathione levels during starvation of cultured astroglial cells. Naunyn-Schmiedeberg's Arch Pharmacol, 358(6), 616-622. doi: 10.1007/pl00005302
Drukarch, B., Schepens, E., Stoof, J. C., & Langeveld, C. H. (1997). Anethole dithiolethione prevents oxidative damage in glutathione-depleted astrocytes. European Journal of Pharmacology, 329(2-3), 259-262. doi: 10.1016/S0014-2999(97)89187-X
Elsayed, D. H., El-Shamy, A. A., Abdelrazek, H. M. A., & El-Badry, D. A. (2019). Effect of genistein on semen quality, antioxidant capacity, caspase-3 expression and DNA integrity in cryopreserved ram spermmatozoa. Small Ruminant Research, 177(1), 50-55. doi: 10.1016/j.smallrumres.2019.06.009
Freire, R. S., Morais, S. M., Catunda, F. E. A., & Pinheiro, D. C. S. N. (2005). Synthesis and antioxidant, anti-inflammatory and gastroprotector activities of anethole and related compounds. Bioorganic & Medicinal Chemistry, 13(13), 4353-4358. doi: 10.1016/j.bmc.2005.03.058
Gu, N.-H., Zhao, W.-L., Wang, G.-S., & Sun, F. (2019). Comparative analysis of mammalian sperm ultrastructure reveals relationships between sperm morphology, mitochondrial functions and motility. Reproductive Biology and Endocrinology, 17(1), 66. doi: 10.1186/s12958-019-0510-y
Igbokwe, A. A., Iyasere, O. S., Sobayo, R. A., Iyasere, S., Animashaun, R. I., Balogun, F. A.,… Daramola, J. O. (2019). Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk extender. Reproduction in Domestic Animals, 54(3), 551-559. doi: 10.1111/rda.13393
Kumaresan, A., Johannisson, A., Al-Essawe, E. M., & Morrell, J. M. (2017). Sperm viability, reactive oxygen species, and DNA fragmentation index combined can discriminate between above- and below-average fertility bulls. Journal of Dairy Science, 100(7), 5824-5836. doi: 10.3168/jds.2016-12484
Luo, T., Wang, F., Weng, S., Chen, H., Kang, H., Wang, J., & Luo, S. (2020) Anethole compromises human sperm function by affecting the sperm intracellular calcium concentration and tyrosine phosphorylation. Reproductive Toxicology, 93(1), 99-105. doi: 10.1016/j.reprotox.2020.01.007
Majzoub, A., & Agarwal, A. (2018). Systematic review of antioxidant types and doses in male infertility: benefits on semen parameters, advanced sperm function, assisted reproduction and live-birth rate. Arab Journal of Urology, 16(1), 113-124. doi: 10.1016/j.aju.2017.11.013
Memon, A. A., Wahid, H., Rosnina, Y., Goh, Y. M., Ebrahimi, M., & Nadia, F. M. (2013). Effect of ascorbic acid concentrations, methods of cooling and freezing on boer goat semen cryopreservation. Reproduction in Domestic Animals, 48(2), 325-330. doi: 10.1111/j.1439-0531.2012.02155.x
Naijian, H. R., Kohram, H., Shahneh, A. Z., & Sharafi, M. (2013). Effects of various concentrations of BSA on microscopic and oxidative parameters of Mahabadi goat semen following the freeze-thaw process. Small Ruminant Research, 113(2-3), 371-375. doi: 10.1016/j.smallrumres.2013.03.015
Nunes, J. F. (1982). Étude des effets du plasma seminal sur la survie in vitro des espermatozöides de bouc. Thèse de doctorat, Paris VI, Paris, França.
Okano, D. S., Penitente, J. M., Fº., Gomez León, V. E., Maitan, P. P., Silveira, C. O., Waddington, B.,… Guimarães, J. D. (2019). In vitro evaluation of cryopreserved bovine sperm and its relation to field fertility in fixedâ€time artificial insemination. Reproduction in Domestic Animals, 54(3), 604-612. doi: 10.1111/rda.13401
Oktay, M., Gulcin, I., & Kufrevioglu, O. (2003). Determination of in vitro antioxidant activity of fennel (Foenicu-lum vulgare) seed extracts. LWT - Food Science and Technology, 36(2), 263-271. doi: 10.10 16/S0023-6438(02)00226-8
Oliveira, R. V., Nunes, J. F., Salgueiro, C. C. M., Cavalcante, J. M. M., Brasil, O. O., & Moura, A. A. A. N. (2011). Avaliação de espermatozoides caprinos congelados em meio à base de água de coco em pó (ACP-101®) ou TRIS. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 63(6), 1295-1302. doi: 10.1590/S0102-09352011000600003
Polzin, G. M., Stanfill, S. B., Brown, C. R., Ashley, D. L., & Watson, C. H. (2007). Determination of eugenol, anethole, and coumarin in the mainstream cigarette smoke of Indonesian clove cigarettes. Food and Chemical Toxicology, 45(10), 1948-1953. doi: 10.1016/j.fct.2007.04.012
Sá, N. A. R., Araújo, V. R., Correia, H. H. V., Ferreira, A. C. A., Guerreiro, D. D., Sampaio, A. M.,... Figueiredo, J. R. (2017). Anethole improves the in vitro development of isolated caprine secondary follicles. Theriogenology, 89(1), 226-234. doi: 10.1016/j.theriogenology.2015.12.014
Silva, S. V., Soares, A. T., Batista, A. M., Almeida, F. C., Nunes, J. F., Peixoto, C. A., & Guerra, M. M. P. (2011). In vitro and in vivo evaluation of ram sperm frozen in tris egg-yolk and supplemented with superoxide dismutase and reduced glutathione. Reproduction, Fertility and Development, 46(5), 874-881. doi: 10.1111/j.1439-0531.2011.01758.x
Souza, C., Brandão, F., Santos, J., Alfradique, V., Brair, V., Prellwitz, L.,... Souza-Fabjan, J. M. (2019) 38 Ram sperm longevity after cryopreservation in extender containing L-carnitine. Reproduction, Fertility and Development, 32(2), 145-145. doi: 10.1071/RDv32n2Ab38
Yea, S. S., Jeong, H. S., Choi, C. Y., Park, K. R., Oh, S., Shin, J. G., & Yun, C. H. (2006). Inhibitory effect of anethole on T-lymphocyte proliferation and interleukin-2 production through down-regulation of the NF-AT and AP-1. Toxicology in Vitro, 20(7), 1098-1105. doi: 10.1016/j.tiv.2006.01.020
Bispo, C. A. S., Pugliesi, G., Galvão, P., Rodrigues, M. T., Ker, P. G., Filgueiras, B., & Carvalho, G. R. (2011). Effect of low and high egg yolk concentrations in the semen extender for goat semen cryopreservation. Small Ruminant Research, 100(1), 54-58. doi: 10.1016/j.smallrumres.2011.05.003
Bucak, M. N., Sariözkan, S., Tuncer, P. B., UlutaÅŸ, P. A., & Akçadaǧ, H. I. (2009). Effect of antioxidants on microscopic semen parameters, lipid peroxidation and antioxidant activities in Angora goat semen following cryopreservation. Small Ruminant Research, 81(2-3), 90-95. doi: 10.1016/jsmallrumres.20 08.11.011
Câmara, T. S., Sousa, A., Jr., Barçante, F. P. S., Silva, J. H. L., Sousa, M. S., Machado, A. A. C.,... Nunes, J. F. (2019). Comparação da qualidade seminal de caprinos das raças Canindé e Alpina Britânica no Nordeste brasileiro. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 71(04), 1260-1268. doi: 10.1590/1678-4162-10297
Castelo Branco, Y. N. T. C., Castelo Branco, M. A., Lustosa, M. S. C., Nascimento, I. M. R., Barros, F. N., Sousa, M. A. C., Fº.,... Souza, J. A. T. (2020). The use of Eugenol in the cryopreservation of bovine sêmen. Brazilian Journal of Development, 6(11), 86336-86355. doi: 10.34117/bjdv6n11-162
Chainy, G. B. N., Manna, S. K., Chaturvedi, M. M., & Aggarwal, B. B. (2000). Anethole blocks both early and late cellular responses transduced by tumor necrosis factor: Effect on NF-κB, AP-1, JNK, MAPKK and apoptosis. Oncogene, 19(25), 2943-2950. doi: 10.1038/sj.onc.1203614
Chelucci, S., Pasciu, V., Succu, S., Addis, D., Leoni, G. G., Manca, M. E., & Berlinguer, F. (2015). Soybean lecithin-based extender preserves spermatozoa membrane integrity and fertilizing potential during goat semen cryopreservation. Theriogenology, 83(6), 1064-1074. doi: 10.1016/j.theriogenology.2014.12.012
Choi, E., & Hwang, J. (2004) Antiinflammatory, analgesic and antioxidant activities of the fruit of Foeniculum vulgare. Fitoterapia, 75(6), 557-565. doi: 10.1016/j.fitote.2004.05.005
Dringen, R., Hamprecht, B., & Drukarch, B. (1998). Anethole dithiolethione, a putative neuroprotectant, increases intracellular and extracellular glutathione levels during starvation of cultured astroglial cells. Naunyn-Schmiedeberg's Arch Pharmacol, 358(6), 616-622. doi: 10.1007/pl00005302
Drukarch, B., Schepens, E., Stoof, J. C., & Langeveld, C. H. (1997). Anethole dithiolethione prevents oxidative damage in glutathione-depleted astrocytes. European Journal of Pharmacology, 329(2-3), 259-262. doi: 10.1016/S0014-2999(97)89187-X
Elsayed, D. H., El-Shamy, A. A., Abdelrazek, H. M. A., & El-Badry, D. A. (2019). Effect of genistein on semen quality, antioxidant capacity, caspase-3 expression and DNA integrity in cryopreserved ram spermmatozoa. Small Ruminant Research, 177(1), 50-55. doi: 10.1016/j.smallrumres.2019.06.009
Freire, R. S., Morais, S. M., Catunda, F. E. A., & Pinheiro, D. C. S. N. (2005). Synthesis and antioxidant, anti-inflammatory and gastroprotector activities of anethole and related compounds. Bioorganic & Medicinal Chemistry, 13(13), 4353-4358. doi: 10.1016/j.bmc.2005.03.058
Gu, N.-H., Zhao, W.-L., Wang, G.-S., & Sun, F. (2019). Comparative analysis of mammalian sperm ultrastructure reveals relationships between sperm morphology, mitochondrial functions and motility. Reproductive Biology and Endocrinology, 17(1), 66. doi: 10.1186/s12958-019-0510-y
Igbokwe, A. A., Iyasere, O. S., Sobayo, R. A., Iyasere, S., Animashaun, R. I., Balogun, F. A.,… Daramola, J. O. (2019). Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk extender. Reproduction in Domestic Animals, 54(3), 551-559. doi: 10.1111/rda.13393
Kumaresan, A., Johannisson, A., Al-Essawe, E. M., & Morrell, J. M. (2017). Sperm viability, reactive oxygen species, and DNA fragmentation index combined can discriminate between above- and below-average fertility bulls. Journal of Dairy Science, 100(7), 5824-5836. doi: 10.3168/jds.2016-12484
Luo, T., Wang, F., Weng, S., Chen, H., Kang, H., Wang, J., & Luo, S. (2020) Anethole compromises human sperm function by affecting the sperm intracellular calcium concentration and tyrosine phosphorylation. Reproductive Toxicology, 93(1), 99-105. doi: 10.1016/j.reprotox.2020.01.007
Majzoub, A., & Agarwal, A. (2018). Systematic review of antioxidant types and doses in male infertility: benefits on semen parameters, advanced sperm function, assisted reproduction and live-birth rate. Arab Journal of Urology, 16(1), 113-124. doi: 10.1016/j.aju.2017.11.013
Memon, A. A., Wahid, H., Rosnina, Y., Goh, Y. M., Ebrahimi, M., & Nadia, F. M. (2013). Effect of ascorbic acid concentrations, methods of cooling and freezing on boer goat semen cryopreservation. Reproduction in Domestic Animals, 48(2), 325-330. doi: 10.1111/j.1439-0531.2012.02155.x
Naijian, H. R., Kohram, H., Shahneh, A. Z., & Sharafi, M. (2013). Effects of various concentrations of BSA on microscopic and oxidative parameters of Mahabadi goat semen following the freeze-thaw process. Small Ruminant Research, 113(2-3), 371-375. doi: 10.1016/j.smallrumres.2013.03.015
Nunes, J. F. (1982). Étude des effets du plasma seminal sur la survie in vitro des espermatozöides de bouc. Thèse de doctorat, Paris VI, Paris, França.
Okano, D. S., Penitente, J. M., Fº., Gomez León, V. E., Maitan, P. P., Silveira, C. O., Waddington, B.,… Guimarães, J. D. (2019). In vitro evaluation of cryopreserved bovine sperm and its relation to field fertility in fixedâ€time artificial insemination. Reproduction in Domestic Animals, 54(3), 604-612. doi: 10.1111/rda.13401
Oktay, M., Gulcin, I., & Kufrevioglu, O. (2003). Determination of in vitro antioxidant activity of fennel (Foenicu-lum vulgare) seed extracts. LWT - Food Science and Technology, 36(2), 263-271. doi: 10.10 16/S0023-6438(02)00226-8
Oliveira, R. V., Nunes, J. F., Salgueiro, C. C. M., Cavalcante, J. M. M., Brasil, O. O., & Moura, A. A. A. N. (2011). Avaliação de espermatozoides caprinos congelados em meio à base de água de coco em pó (ACP-101®) ou TRIS. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 63(6), 1295-1302. doi: 10.1590/S0102-09352011000600003
Polzin, G. M., Stanfill, S. B., Brown, C. R., Ashley, D. L., & Watson, C. H. (2007). Determination of eugenol, anethole, and coumarin in the mainstream cigarette smoke of Indonesian clove cigarettes. Food and Chemical Toxicology, 45(10), 1948-1953. doi: 10.1016/j.fct.2007.04.012
Sá, N. A. R., Araújo, V. R., Correia, H. H. V., Ferreira, A. C. A., Guerreiro, D. D., Sampaio, A. M.,... Figueiredo, J. R. (2017). Anethole improves the in vitro development of isolated caprine secondary follicles. Theriogenology, 89(1), 226-234. doi: 10.1016/j.theriogenology.2015.12.014
Silva, S. V., Soares, A. T., Batista, A. M., Almeida, F. C., Nunes, J. F., Peixoto, C. A., & Guerra, M. M. P. (2011). In vitro and in vivo evaluation of ram sperm frozen in tris egg-yolk and supplemented with superoxide dismutase and reduced glutathione. Reproduction, Fertility and Development, 46(5), 874-881. doi: 10.1111/j.1439-0531.2011.01758.x
Souza, C., Brandão, F., Santos, J., Alfradique, V., Brair, V., Prellwitz, L.,... Souza-Fabjan, J. M. (2019) 38 Ram sperm longevity after cryopreservation in extender containing L-carnitine. Reproduction, Fertility and Development, 32(2), 145-145. doi: 10.1071/RDv32n2Ab38
Yea, S. S., Jeong, H. S., Choi, C. Y., Park, K. R., Oh, S., Shin, J. G., & Yun, C. H. (2006). Inhibitory effect of anethole on T-lymphocyte proliferation and interleukin-2 production through down-regulation of the NF-AT and AP-1. Toxicology in Vitro, 20(7), 1098-1105. doi: 10.1016/j.tiv.2006.01.020
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2021-08-12
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Sousa, M. S., Brito, B. F., Novaes, M. A. S., Câmara, T. S., Paula, N. R. de O., Salgueiro, C. C. de M., & Nunes, J. F. (2021). Effect of adding anethole to the cryopreservation medium (powdered coconut water) on morphology, kinetics, and oxidative stress of buck sperm. Semina: Ciências Agrárias, 42(6), 3235–3246. https://doi.org/10.5433/1679-0359.2021v42n6p3235
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