Use of natural antioxidants in in vitro mammalian embryo production

Authors

  • Maria Valéria de Oliveira Santos Universidade Federal Rural do Semi-Árido
  • Alana Azevedo Borges Universidade Federal Rural do Semi-Árido
  • Luiza Bento de Queiroz Neta Universidade Federal Rural do Semi-Árido
  • Luciana Medeiros Bertini Instituto Federal do Rio Grande do Norte
  • Alexsandra Fernandes Pereira Universidade Federal Rural do Semi-Árido

DOI:

https://doi.org/10.5433/1679-0359.2018v39n1p431

Keywords:

Culture medium, Natural bioactive, Oxidative stress, Reproduction.

Abstract

In vitro embryo production (IVEP) contributes to the quantitative and qualitative aspects of animal reproduction. Nevertheless, inherent technical factors such as oxidative stress can negatively influence the result and this can impair cell metabolism, thus decreasing the rates of in vitro development, and necessitating the supplementation of culture medium with antioxidants. In this context, compounds of natural origin with this property have been highlighted because of the positive results obtained at different stages of IVEP. Thus, this review aims to present the results obtained by using natural antioxidants to minimize the effects of oxidative stress on gametes and embryos. A variety of natural isolated substances and mixtures (essential oils and extracts) have been studied for supplementation of IVEP media, at stages of in vitro maturation, sperm capacitation, in vitro fertilization, and in vitro development of embryos in different mammalian species. Generally, beneficial effects are observed according to the concentration used, thus demonstrating the potential of several natural antioxidants. Therefore, the main challenges in using these compounds as antioxidants during IVEP include proving their efficiency against free radicals and determining the best concentration at each stage. In addition, understanding the mechanisms of action of such antioxidants is crucial to establishing their use in IVEP biotechnology.

Author Biographies

Maria Valéria de Oliveira Santos, Universidade Federal Rural do Semi-Árido

Discente, Curso de Mestrado, Programa de Pós-Graduação em Ciência Animal, PPGCA, Universidade Federal Rural do Semi-Árido, UFERSA, Mossoró, RN, Brasil.

Alana Azevedo Borges, Universidade Federal Rural do Semi-Árido

Discente, Curso de Doutorado, PPGCA/UFERSA, Mossoró, RN, Brasil.

Luiza Bento de Queiroz Neta, Universidade Federal Rural do Semi-Árido

Discente, Curso de Mestrado, Programa de Pós-Graduação em Ciência Animal, PPGCA, Universidade Federal Rural do Semi-Árido, UFERSA, Mossoró, RN, Brasil.

Luciana Medeiros Bertini, Instituto Federal do Rio Grande do Norte

Profa, Instituto Federal do Rio Grande do Norte, IFRN, Apodi, RN, Brasil.

Alexsandra Fernandes Pereira, Universidade Federal Rural do Semi-Árido

Profa, UFERSA, Mossoró, RN, Brasil.

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Published

2018-02-16

How to Cite

Santos, M. V. de O., Borges, A. A., Queiroz Neta, L. B. de, Bertini, L. M., & Pereira, A. F. (2018). Use of natural antioxidants in in vitro mammalian embryo production. Semina: Ciências Agrárias, 39(1), 431–444. https://doi.org/10.5433/1679-0359.2018v39n1p431

Issue

Section

Review Article

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