Isolation and identification of Mycobacterium bovis in cattle slaughtered from an abattoir in Garanhuns, Pernambuco

Authors

  • Stephanie Caroline Gueiros Silva Universidade Federal Rural de Pernambuco
  • Amanda de Deus Ferreira Alves Universidade Federal Rural de Pernambuco
  • Tássio José de Oliveira Almeida Universidade Federal Rural de Pernambuco
  • Pamella Christhine Lins de Sá Universidade Federal Rural de Pernambuco
  • Antonio Franciso de Souza Filho Universidade de São Paulo
  • Nicolás Céspedes Cárdenas Universidade de São Paulo
  • José Soares Ferreira Neto Universidade de São Paulo
  • Marcos Bryan Heinemann Universidade de São Paulo
  • Sérgio Santos de Azevedo Universidade Federal da Paraíba
  • Márcia Bersane Araujo de Medeiros Torres Universidade Federal Rural de Pernambuco

DOI:

https://doi.org/10.5433/1679-0359.2018v39n1p157

Keywords:

Granuloma, Pneumonia, Public health.

Abstract

Tuberculosis is a chronic infectious disease caused by the Mycobacterium tuberculosis complex (CMT), members including M. bovis. The sanitary inspection in slaughterhouses has a great importance on public health for the removal of contaminated meat with pathological lesions. Cattle slaughtered in the abattoir of Garanhuns were subjected to macroscopic, histological, bacteriological, and molecular analyses. Lung inspection revealed gross lesions suggestive of tuberculosis. The characterization of tuberculous granulomas was performed by histopathology and hematoxylin and eosin (HE) staining. The identification of acid-fast bacilli (AFB) was performed on smears with fresh material and histological staining using Ziehl-Neelsen stain. Bacteriological diagnosis was carried out using Stonebrink and Lowenstein-Jensen media, and polymerase chain reaction (PCR) was performed from the DNA extracted from colonies for the identification of Mycobacterium spp. We found that 1% (32/3,.180) of the collected lungs showed lesions suggestive of tuberculosis. Histopathological analysis revealed that 65.62% (21/32) samples had granulomatous pneumonia. AFB was detected in 46.88% (15/32) smears and 4.16% (1/24) histological sections, as analyzed by Ziehl-Neelsen staining. Moreover, 68.75% (22/32) samples showed bacterial growth and molecular identification 90% (18/20) of the samples were positive for M. bovis and 10% (2/20) for Mycobacterium sp. Thus, microbiological culture and PCR analyses revealed the high rate of bovine tuberculosis in this region, indicative of the need to disclose these results in the interest of public health. Consequently, we emphasize the need for an accurate macroscopic evaluation of lesions suggestive of bovine tuberculosis.

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Author Biographies

Stephanie Caroline Gueiros Silva, Universidade Federal Rural de Pernambuco

Discente de Doutorado, Universidade Federal Rural de Pernambuco, UFRPE, Recife, PE, Brasil.

Amanda de Deus Ferreira Alves, Universidade Federal Rural de Pernambuco

Discente de Mestrado, UFRPE, Recife, PE, Brasil.

Tássio José de Oliveira Almeida, Universidade Federal Rural de Pernambuco

Discente de Graduação, Unidade Acadêmica de Garanhuns, UFRPE/UAG, Garanhuns, PE, Brasil.

Pamella Christhine Lins de Sá, Universidade Federal Rural de Pernambuco

Médica Veterinária Autônoma, Unidade Acadêmica de Garanhuns, UFRPE/UAG, Garanhuns, PE, Cidade, UF, Brasil.

Antonio Franciso de Souza Filho, Universidade de São Paulo

Discente de Doutorado, Universidade de São Paulo, USP, São Paulo, SP, Brasil.

Nicolás Céspedes Cárdenas, Universidade de São Paulo

Discente de Mestrado, USP, São Paulo, SP, Brasil.

José Soares Ferreira Neto, Universidade de São Paulo

Prof. Titular, USP, São Paulo, SP, Brasil.

Marcos Bryan Heinemann, Universidade de São Paulo

Prof. Dr., USP, São Paulo, SP, Brasil.

Sérgio Santos de Azevedo, Universidade Federal da Paraíba

Prof. Associado, Universidade Federal da Paraíba, UFPB, Patos, PB, Brasil.

Márcia Bersane Araujo de Medeiros Torres, Universidade Federal Rural de Pernambuco

Profa Adjunto IV, Unidade Acadêmica de Garanhuns, UFRPE/UAG, Garanhuns, PE, Brasil.

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Published

2018-02-16

How to Cite

Silva, S. C. G., Alves, A. de D. F., Almeida, T. J. de O., Sá, P. C. L. de, Souza Filho, A. F. de, Cárdenas, N. C., … Torres, M. B. A. de M. (2018). Isolation and identification of Mycobacterium bovis in cattle slaughtered from an abattoir in Garanhuns, Pernambuco. Semina: Ciências Agrárias, 39(1), 157–166. https://doi.org/10.5433/1679-0359.2018v39n1p157

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