Ice nucleation activity in Pantoea ananatis obtained from maize white spot lesions

Autores

  • Aline Vanessa Sauer Universidade Estadual de Londrina
  • Kátia Real Rocha Universidade Estadual de Londrina
  • Eliseu dos Santos Pedro Universidade Estadual de Londrina
  • Walter Fernandes Meirelles Embrapa Milho e Sorgo
  • Luzia Doretto Paccola-Meirelles Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/1679-0359.2014v35n4p1659

Palavras-chave:

INA, Vesicle protein, Transmission electron microscopy.

Resumo

Maize white spot lesions caused by Pantoea ananatis has contributed substantially to yield reduction of maize crops in many countries, including Brazil. The initial symptoms of the disease include water-soaked lesions on the leaves, which later become necrotic and straw-colored. Basic knowledge regarding the biology and the infection mechanisms of this pathogen is lacking. In this study, 15 P. ananatis isolates obtained from maize white spot lesions were examined for their ice nucleation activity (INA). The INAs of individual bacterial isolates was determined by tube nucleation tests. Bacterial isolates were grown on tryptic soy broth medium and an aliquot of 0.1 mL of culture was added to test tubes containing 1 mL of sterile distilled water. The tubes were packed in an ice bath, which had a temperature below –10°C, for approximately 2 min. Instantaneous formation of ice in the tube revealed a positive INA phenotype of the isolate. Only 9 of the 15 studied isolates showed the INA+ phenotype. Pathogenicity tests were performed using whole plants and detached leaves. Symptoms were reproduced in both tests, but only for the inoculations using INA+ isolates. Electron microscopy allowed visualization of protein vesicles under outer cell wall of isolates characterized as INA+.

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Biografia do Autor

Aline Vanessa Sauer, Universidade Estadual de Londrina

Engª Agrª, Discente de Mestrado em Agronomia, Centro de Ciências Agrárias, Programa de Pós Graduação em Agronomia, Universidade Estadual de Londrina, UEL, Londrina, PR, Brasil. Bolsista CNPq,

Kátia Real Rocha, Universidade Estadual de Londrina

Discente de Graduação, UEL, Londrina, PR, Brasil. Bolsista CNPq.

Eliseu dos Santos Pedro, Universidade Estadual de Londrina

Discente de Graduação, UEL, Londrina, PR, Brasil. Bolsista CNPq.

Walter Fernandes Meirelles, Embrapa Milho e Sorgo

Engo Agro, Dr. Pesquisador, Empresa Brasileira de Pesquisa Agropecuária, Embrapa Milho e Sorgo, Sete Lagoas, MG, Brasil.

Luzia Doretto Paccola-Meirelles, Universidade Estadual de Londrina

Bióloga, Profa Dra Pesquisador Senior, Deptº de Biologia Geral, CCB, UEL, Londrina, PR, Brasil.

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Publicado

2014-08-27

Como Citar

Sauer, A. V., Rocha, K. R., Pedro, E. dos S., Meirelles, W. F., & Paccola-Meirelles, L. D. (2014). Ice nucleation activity in Pantoea ananatis obtained from maize white spot lesions. Semina: Ciências Agrárias, 35(4), 1659–1666. https://doi.org/10.5433/1679-0359.2014v35n4p1659

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