Characterization and cultivation of a wild mushroom species isolated in Brazil

Authors

  • Cristina Sayuri Maki Universidade de São Paulo
  • Luzia Doretto Paccola-Meirelles Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/1679-0367.2002v23n1p77

Abstract

Wild mushrooms were collected close to cattle manure in pasture areas in Tamarana (Paraná/Brazil), with the objective of finding and domesticating new non-exploited basidiomycetes. An edible basidiomycete of the Agaricales order was classified as belonging to the Macrolepiota bonaerensis species (=Lepiota procera, form bonaerensis (Speg.) Rick or M. procera (Scop.:Fr) Sing.). The mycelia was isolated and characterized for growth rate in different culture media. A vigorous growth was observed in PDA Medium (pH 5, ±25°C). The oat culture medium was the most appropriate for cultivation submersed. M. bonaerensis mycelia secretes lipase, cellulase and protease exoenzyme types. Cytological analyses confirmed the bi-nucleated condition of the mycelia, the presence of septa and clamp connections in the hypha. The fructification occurred in the substrate composed of sterilized humus + soil (1:9) plus casing layer with active carbon.

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Author Biographies

Cristina Sayuri Maki, Universidade de São Paulo

Doutoranda em Genética e Melhoramento de Plantas pela Escola Superior de Agricultura “Luiz de Queiroz”/USP.

Luzia Doretto Paccola-Meirelles, Universidade Estadual de Londrina

 

Professor Associado da Universidade Estadual de Londrina - Departamento de Biologia Geral.

Published

2004-07-15

How to Cite

1.
Maki CS, Paccola-Meirelles LD. Characterization and cultivation of a wild mushroom species isolated in Brazil. Semin. Cienc. Biol. Saude [Internet]. 2004 Jul. 15 [cited 2024 Oct. 2];23(1):77-82. Available from: https://ojs.uel.br/revistas/uel/index.php/seminabio/article/view/3697

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Section

Artigos