Sequencial passages of the multiple nucleopolyhedrovirus of Anagrapha falcifera(Kirby) (Lepidoptera: Noctuidae) (AfMNPV) in Spodoptera cosmioides (Walker) (Lepidoptera, Noctuidae)

Maurício Lara Moscardi; Viviane Dutra; Pablo Kruger Fernandes; Samuel Roggia; Sheila Michele Levy; Angela Maria Ferreira Falleiros

doi:10.5433/1679-0367.2021v42n2p221

Authors

Maurício Lara Moscardi Universidade Estadual de Londrina - UEL https://orcid.org/0000-0001-5300-699X
Viviane Dutra Universidade Estadual de Londrina - UEL https://orcid.org/0000-0002-3520-5001
Pablo Kruger Fernandes Universidade Estadual de Londrina - UEL https://orcid.org/0000-0001-7614-3487
Samuel Roggia Empresa Brasileira de Pesquisa Agropecuária - Embrapa soja https://orcid.org/0000-0002-9707-1766
Sheila Michele Levy Universidade Estadual de Londrina - UEL https://orcid.org/0000-0002-3043-7655
Angela Maria Ferreira Falleiros Universidade Estadual de Londrina - UEL https://orcid.org/0000-0002-8982-9709

DOI:

https://doi.org/10.5433/1679-0367.2021v42n2p221

Keywords:

Morphology, Histopathology, Biological control, Insect viruses

Abstract

The aim of this work was to evaluate the effects of Anagrapha falcifera (AfMNPV) multiple nucleopolyhedrovirus passages in the S. cosmioides caterpillar’s biology at different times of infection and histological changes that the virus could cause in the caterpillar midgut, seeking correlate histopathologic effects to the effectiveness of this virus as a potential biological control of this pest. Larvae were infected with seven days of development, by using three different passages of AfMNPV on S. cosmioides (F1, F4 and F7, which is the first, fourth and seventh passages, respectively) and the control treatment. Compared biology assays with the same treatments for analyzing behavior and mortality of caterpillars were performed concomitantly. The midgut morphology was compared between infected and uninfected larvae. The digestive tubes were collected at 24, 72 and 144 hours of infection (20 tubes/treatment/time of infection). After collection, the digestive tubes were fixed in Karnovsky, processed, stained with Hematoxylin-Eosin, and examined under a light microscope. The biology results of F4 and F7treatments, showed a drastic reduction in locomotion and feeding from the fourth day after infection and higher cumulative mortality rate compared to the control and F1. All treatments caused morphological changes in the midgut of S. cosmioides, in the three times of infection, with the greatest changes occurring at the epithelium. The AfMNPV, in the three passages tested in S. cosmioides, caused behavioral and morphological changes in the midgut, indicating that it can be a promising agent for biological control of this pest.

Author Biographies

Maurício Lara Moscardi, Universidade Estadual de Londrina - UEL

Doctoral candidate in Agronomy (Phytosanitary/Entomology) by the Graduate Program of the Universidade Estadual de Londrina, Londrina, Paraná, Brazil.

Viviane Dutra, Universidade Estadual de Londrina - UEL

Doctorate in Agronomy (Phytosanitary/Entomology) by the Graduate Program of the Universidade Estadual de Londrina, Londrina, Paraná, Brazil. Technical Manager, Andermatt do Brasil Soluções Biológica, Curitiba, Paraná

Pablo Kruger Fernandes, Universidade Estadual de Londrina - UEL

Biólogo pela Universidade Estadual de Londrina, Londrina, Paraná

Samuel Roggia, Empresa Brasileira de Pesquisa Agropecuária - Embrapa soja

PhD in Entomology from the Luiz de Queiros School of Agriculture, Universidade def São Paulo, Piracicaba. Researcher at Embrapa Soja, Entomology Sector, Londrina, Paraná

Sheila Michele Levy, Universidade Estadual de Londrina - UEL

Doctorate in Biological Sciences (Zoology) from Universidade Estadual Paulista - Júlio de Mesquita Filho, Botucatu. Professor, Department of Histology, Center for Biological Sciences, SUniversidade Estadual de Londrina, Londrina, Paraná

Angela Maria Ferreira Falleiros, Universidade Estadual de Londrina - UEL

Doctorate in Biological Sciences (Human Anatomy) from Universidade Estadual Paulista - Júlio de Mesquita Filho, Botucatu, São Paulo. Professor, Department of Histology, Center for Biological Sciences, Universidade Estadual de Londrina.

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