Leishmanicidal activity of brazilian propolis hydroalcoholic extract in Leishmania amazonensis

Authors

  • Suelen Santos da Silva Universidade Estadual de Londrina - UEL
  • Milena Menegazzo Miranda Universidade Estadual de Londrina - UEL
  • Idessania Nazareth Costa Universidade Estadual de Londrina - UEL
  • Maria Angélica Ehara Watanabe Universidade Estadual de Londrina - UEL
  • Wander Rogério Pavanelli Universidade Estadual de Londrina - UEL
  • Ionice Felipe Universidade Estadual de Londrina - UEL
  • José Mauricio Sforcin Universidade Estadual Paulista, UNESP,
  • Ivete Conchon-Costa Universidade Estadual de Londrina - UEL

DOI:

https://doi.org/10.5433/1679-0367.2015v36n2p25

Keywords:

Cutaneous leishmaniasis, Propolis, Leishmania.

Abstract

Leishmaniosis are considered neglected diseases due to its high incidence, widespread and difficulty in treatment being included in the list of priority diseases by the World Health Organization. Available treatments for these diseases have high toxicity, which explains the search for more effective drugs. Previous studies with propolis - a resinous substance produced by bees - demonstrated immunomodulatory and anti-parasitic activity in several experimental models. The objective of this study was to evaluate the effect in vitro of Brazilian propolis hydroalcoholic extract, collected in the city of Botucatu in São Paulo State, on promastigotes forms of Leishmania amazonensis as well as its effect on the parasite load in the spleen of infected mice. Thus, promastigote forms treated with 5, 10, 25, 50 or 100 ?g/mL of Brazilian propolis hydroalcoholic extract at 24, 96 and 168 hours showed inhibitory effect on the spread of these pararasite at all indicated times. However, the concentrations of 50 and 100 ?g/mL were more effective, reducing the parasite spread when compared to the control and other concentrations at all times. Regarding parasitic load, after 30 days of infection with L. amazonensis, BALB/c mice were treated on a daily basis with propolis (5mg/kg) orally or intraperitoneally for 60 days. Further, the spleen was collected for parasite load analysis. Oral treatment reduced 40% of the parasitic load. Thus, the tested Brazilian propolis sample showed antileishmanial activity on L. amazonensis in culture and in parasite- infected mice.

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Author Biographies

Suelen Santos da Silva, Universidade Estadual de Londrina - UEL

PhD student of Experimental Pathology Program, State University of Londrina, Parana, Brazil.

Milena Menegazzo Miranda, Universidade Estadual de Londrina - UEL

PhD student of Experimental Pathology Program, State University of Londrina, Parana, Brazil.

Idessania Nazareth Costa, Universidade Estadual de Londrina - UEL

PhD in Immunology and Parasitology Applied by the Federal University of Uberlândia. Assistant Professor, Department of Pathological Sciences, Parasitology area, Centre for Biological Sciences (CCB), State University of Londrina, Parana, Brazil.

Maria Angélica Ehara Watanabe, Universidade Estadual de Londrina - UEL

PhD in Biological Sciences, Biochemistry. Assistant Professor, Department of Pathological Sciences, Immunology Basic area, Center of Biological Sciences (CCB), State University of Londrina, Parana, Brazil.

Wander Rogério Pavanelli, Universidade Estadual de Londrina - UEL

PhD in Immunology from the University of São Paulo-USP. Teaching assistant at the Department of Pathological Sciences, Parasitology area, Centre for Biological Sciences (CCB), State University of Londrina, Parana, Brazil.

Ionice Felipe, Universidade Estadual de Londrina - UEL

PhD in Biological Sciences (Biophysics) from the Federal University of Rio de Janeiro. Assistant Professor, Department of Pathological Sciences, Immunology Basic area, Center of Biological Sciences (CCB), State University of Londrina, Parana, Brazil.

José Mauricio Sforcin, Universidade Estadual Paulista, UNESP,

PhD in Nutrition and Animal Production, FMVZ, UNESP, Botucatu. Associate Professor, Department of Microbiology and Immunology, University Estadual Paulista, UNESP, Botucatu, São Paulo, Brazil.

Ivete Conchon-Costa, Universidade Estadual de Londrina - UEL

PhD in Microbiology from the State University of Londrina. Assistant Professor, Department of Pathological Sciences, Immunology Basic area, Center of Biological Sciences (CCB), State University of Londrina, Parana, Brazil.

Published

2016-02-11

How to Cite

1.
Silva SS da, Miranda MM, Costa IN, Watanabe MAE, Pavanelli WR, Felipe I, et al. Leishmanicidal activity of brazilian propolis hydroalcoholic extract in Leishmania amazonensis. Semin. Cienc. Biol. Saude [Internet]. 2016 Feb. 11 [cited 2024 Nov. 21];36(2):25-34. Available from: https://ojs.uel.br/revistas/uel/index.php/seminabio/article/view/22621

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