Analysis of genetic polymorphism of FOXP3 (forkhead Box P3 [Homo sapiens]) in healthy individuals of Northern Paraná

Abstract

Regulatory T cells (Tregs) play a central role in induction and maintenance of immunological tolerance. These cells express a transcription factor called forkhead transcription factor 3 (FOXP3) in which genetic polymorphisms can lead to lack of function of these cells. Thus, the objective of this study was to evaluate the occurrence of polymorphisms -924 (A/G, rs2232365) and -3279 (C/A, rs3761548) of the FOXP3 gene in the population of Northern of Paraná. Polymorphisms were amplified by polymerase chain reaction allele-specific in 115 women healthy donors and the analysis was performed by polyacrylamide gel electrophoresis. The population of Northern of Paraná showed 8.70% of genotype AA, 49.56% AC and 41.74% CC, for the -3279 polymorphism, while for the -924 polymorphism, the occurrence of subjects was 34.78% for genotype AA, 28.70% AG and 36.52% GG. The presence of allele A -3279 polymorphism was 33.48% and the allele C was 66.52%. For the polymorphism -924, the occurence of allele A was 49.13% and to allele G was 50.87%. It is hoped that the results of this study contribute in the development of studies of association between genetic polymorphisms of the FOXP3 gene, since the establishment of genotyping of the population control is crucial in the analysis.