Estudo genético de fatores de patogenicidade em Escherichia coli isoladas de suínos na região de Londrina, PR.

Abstract

The production of heat-stable (STa) and heat-labile (LT) enterotoxins, K88 and K99 colonization factors were studied in 25 E. coli strains isolated from diarrhoeal piglets. The production of hemolysin, colicin V, drug resistance characters of the same strains and the transference of Col V, STa, Hly and K88 plasmids were also analyzed by conjugation. 9(36%) strains produced both hemolisin and colicin V, 4(16%) LT, 3(12%) ST and 3(12%) K88. 24 (96%) strains were resistant to antibiotic, with 21 (84%) of them showing multiple resistance. The antibiotic resistance markers and colicin, 12(50%) and 5(55%) strains were capable of transferring these characteristics. After studding 3 STa producing strains, is was seen that in one of them STa genes were located along with colicin V on a conjugative plasmid. In the other strains the STa plasmid was transferred simultaneously with the plasmid earring colicin V and drug resistance gene agaisnt tetracycline and streptomycin. Genetic information coding the production of hemolision and K88 were not in plasmid conjugative.