Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense
DOI:
https://doi.org/10.5433/1679-0359.2020v41n5supl1p2401Keywords:
Panama disease, Musa spp., biological control.Abstract
Detached plant organs are alternative materials to in vitro tests for selecting biocontrol agents. On the other hand, the use of scales to quantify injured areas can generate inconsistent results. Rhizospheric and endophytic bacteria were selected as growth inhibitors of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Panama disease of banana. For this, rhizome discs were treated with 200 µL of antagonist suspension (109 CFU mL?1) and inoculated with the pathogen. The material was placed in plastic gerbox boxes and incubated in BOD at 25 °C for 12 days. Afterward, the area of rhizome discs (mm2) colonized by Foc was quantified by digital images. The assay was set up in a completely randomized design, with four replications and three discs per replication. The control consisted of untreated and inoculated discs. The results showed the efficiency of this method in selecting the biological control agent, as the 26 isolates were group into five different clusters, with isolates belonging to four of these groups (from ‘1’ to ‘4’) being able to reduce Foc colonization. Isolates 520EB, 993EB, and 531EB had the highest potential for inhibition, with areas of 343.3, 344.1, 364.8 mm2, respectively, promoting inhibition ranging from 53 to 56 % of the colonized area compared to the control (782.6 mm2).Downloads
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References
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Ploetz, R. C. (2015). Fusarium wilt of banana. Phytopathology, 105(12), 1512-1521. doi: 10.1094/PHYTO-04-15-0101-RVW
Rajkumar, M., Lee, W. U., & Lee, K. J. (2005). Screening of bacterial antagonists for biological control of Phytophthora blight of pepper. Journal of Basic Microbiology, 45(1), 55-63. doi: 10.1002/jobm. 200410445
Ramamoorthy, V., Viswanathan, R., Raguchander, T., Prakasam, V., & Samiyappan, R. (2001). Induction of systemic resistance by plant growth promoting rhizobacteria in crop plants against pests and diseases. Crop Protection, 20(1), 1-11.
R Core Development Team (2017). R: A Language and Environment for Statistical Computing. Retrieved from http://www.R-project.org
Renwick, A., Campbell, R., & Coe, S. (1991). Assessment of in vitro screening systems for potential biocontrol agents of Gaeumannomyces graminis. Plant Pathology, 40(4), 524-532. doi: 10.1111/j.1365-3059.1991.tb02415.x
Romeiro, R. S. (2007). Controle biológico de enfermidades de plantas: procedimentos. Viçosa, MG: Ed. UFV.
Shiomi, H. F., Silva, H. S. A., Melo, I. S., Nunes, F. V., & Bettiol, W. (2006). Bioprospecting endophytic bacteria for biological control of coffee leaf rust. Scientia Agricola, 63(1), p. 32-39. doi: 10.1590/S0103-90162006000100006
Silva, H. S. A., Tozzi, J. P. L., Terrasan, C. R. F., & Bettiol, W. (2012). Endophytic microrganismos from coffee tissues as plant growth promoters and bicontrol agents of coffee leaf rust. Biological Control, 63, 62-67. doi: 10.1016/j.biocontrol.2012.06.005
Silva, H. S. A., Oliveira, S. A. S., & Haddad, F. (2011). Uso de imagens digitalizadas em metodologias de seleção para resistência à podridão radicular de mandioca. (Boletim de Pesquisa e Desenvolvimento, 54). Cruz das Almas, BA: EMBRAPA Mandioca e Fruticultura Recuperado de http://www.cnpmf. embrapa.br/publicacoes/boletins/ boletim_54.pdf
Snyder, W. C. & Hansen, H. N. (1953). Species concept, genetics, and pathogenicity in Hypomyces solani. Phytopathology, 44(2), 338-342.
Spadaro, D., & Gullino, M. L. (2005). Improving the efficacy of biocontrol agents against soilborne pathogens. Crop Protection, 24(7), 601-613. doi: 10.1016/j.cropro.2004.11.003
Szandala, E. S., & Backhouse, D. (2001). Suppression of sporulation of Botrytis cinerea by antagonists applied after infection. Australasian Plant Pathology, 30(2), 165-170.
Zhang, X., Zhou, Y., Li, Y., Fu, X., & Wang, Q. (2017). Screening and characterization of endophytic Bacillus for biocontrol of grapevine downy mildew. Crop Protection, 96, 173-179. doi: 10.1016/ j.cropro.2017.02.018
Zheng, Y., Xue, Q.-Y., Xu, L.-L., Xu, Q., Lu, S., Gu, C., & Guo, J.-H. (2011). A screening strategy of fungal biocontrol agents towards Verticillium wilt cotton. Biological Control, 56, 209-2016. doi: 10.1016/j.biocontrol.2010.11.010
Benchimol, R. L., Chu, E. Y., Yuitimuto, R., & Dias, M. B., Fº. (2000). Controle da fusariose em plantas de pimenta-do-reino com bactérias endofíticas: sobrevivência e respostas morfofisiológicas. Pesquisa Agropecuária Brasileira, 35(7), 1343-348.
Beyer, M., Röding, S., Ludewig, A., & Verreet, J.-A. (2004). Germination and survival of Fusarium graminearum macroconidia as affected by environmental factors. Journal of Phytopathology, 152(2), 92-97. doi: 10.1111/j.1439-0434.2003.00807.x
Bharat, R., Singh, V. N., & Singh, D. B. (1980). Trichoderma viride as a mycoparasite of Aspergillus spp. Plant and Soil, 57(1), 131-135.
Burmeister, L., & Hau, B. (2009). Control of the bean rust fungus Uromyces appendiculatus by means of Trichoderma harzianum: leaf disc assays on the antibiotic effect of spore suspensions and culture filtrates. BioControl, 54(4), 575-585. doi: 10.1007/s10526-008-9202-9
Chen, Y., Mei, R., Liu, L., & Kloepper, J. W. (1996). The use of yield increasing bacteria (YIB) as plant growth-promoting rhizobacteria in Chinese agriculture. In R. S. Utkhede, & V. K. Gupta (Eds.), Management of soil born diseases (pp. 165-184). Ludhiana: Kalyani Publishers.
Cordeiro, Z. J. M., Matos, A. P., & Meissner, P. E., Fº. (2004). Doenças e métodos de controle. In A. L. Borges, & L. S. Souza (Eds.), O cultivo da bananeira (pp. 146-182). Cruz das Almas, BA: EMBRAPA Mandioca e Fruticultura.
Eljounaidi, K., Lee, S. K., & Bae, H. (2016). Bacterial endophytes as potential biocontrol agents of vascular wilt diseases - review and future prospects. Biological Control, 103, 62-68. doi: 10.1016/j.biocontrol. 2016.07.013
Food and Agriculture Organization of the United Nations (2018). Production crops. Recuperado de http://faostat.fao.org/site/339/default.aspx
Fiddaman, P. J., O’Neill, T. M., & Rossall, S. (2000). Screening of bacteria for the suppression of Botrytis cinerea and Rhizoctonia solani on lettuce (Lactuca sativa) using leaf disc bioassays. Annals of Applied Biology, 137(3), 223-235.
Fravel, D. R. (2005). Commercialization and implementation of biocontrol. Annual Review of Phytopathology, 43, 337-359. doi: 10.1146/annurev.phyto.43.032904.092924
Helbig, J. (2001). Biological control of Botrytis cinerea Pers. ex Fr. in strawberry by Paenibacillus polymyxa (Isolate 18191). Journal of Phytopathology, 149(5), 265-273. doi: 10.1046/j.1439-0434.2001.00609.x
Idris, H. A., Labuschagne, N., & Korsten, L. (2007). Screening rhizobacteria for biological control of Fusarium root and crown rot of sorghum in Ethiopia. Biological Control, 40 (1), 97-106. doi: 10.1016/j.biocontrol.2006.07.017
Knudsen, I. M. B., Hockenhull, J., Funk Jensen, D., Gerhardson, B., Hökeberg, M., Tahvonen, R., Teperi, R., Sundheim, L., & Henriksen, B. (1997) Selection of biological control agents for controlling soil and seed-borne diseases in field. European Journal of Plant Pathology, 103(9), 775-784.
Köhl, J., Postma, J., Nicot, P., Ruocco, M., & Blum, B. (2011). Stepwise screening of microorganisms for commercial use in biological control of plant-pathogenic fungi and bacteria. Biological Control, 57(1), 1-12. doi: 10.1016/j.biocontrol.2010.12.004
Lian, J., Wang, Z., Cao, L., Tan, H., Inderbitzin, P., Jiang, Z., & Zhou, S. (2009). Artificial inoculation of banana tissue culture plantlets with indigenous endophytes originally derived from native banana plants. Biological Control, 51(3), 427-434. doi: 10.1016/j.biocontrol.2009.08.002
Luquine, L. S. (2012). Aplicação conjunta de rizobactérias e bactérias endofíticas para o biocontrole do Mal-do-panamá da bananeira. Dissertação de mestrado, Universidade Federal do Recôncavo da Bahia, Cruz das Almas, BA, Brasil.
Ploetz, R. C. (2015). Fusarium wilt of banana. Phytopathology, 105(12), 1512-1521. doi: 10.1094/PHYTO-04-15-0101-RVW
Rajkumar, M., Lee, W. U., & Lee, K. J. (2005). Screening of bacterial antagonists for biological control of Phytophthora blight of pepper. Journal of Basic Microbiology, 45(1), 55-63. doi: 10.1002/jobm. 200410445
Ramamoorthy, V., Viswanathan, R., Raguchander, T., Prakasam, V., & Samiyappan, R. (2001). Induction of systemic resistance by plant growth promoting rhizobacteria in crop plants against pests and diseases. Crop Protection, 20(1), 1-11.
R Core Development Team (2017). R: A Language and Environment for Statistical Computing. Retrieved from http://www.R-project.org
Renwick, A., Campbell, R., & Coe, S. (1991). Assessment of in vitro screening systems for potential biocontrol agents of Gaeumannomyces graminis. Plant Pathology, 40(4), 524-532. doi: 10.1111/j.1365-3059.1991.tb02415.x
Romeiro, R. S. (2007). Controle biológico de enfermidades de plantas: procedimentos. Viçosa, MG: Ed. UFV.
Shiomi, H. F., Silva, H. S. A., Melo, I. S., Nunes, F. V., & Bettiol, W. (2006). Bioprospecting endophytic bacteria for biological control of coffee leaf rust. Scientia Agricola, 63(1), p. 32-39. doi: 10.1590/S0103-90162006000100006
Silva, H. S. A., Tozzi, J. P. L., Terrasan, C. R. F., & Bettiol, W. (2012). Endophytic microrganismos from coffee tissues as plant growth promoters and bicontrol agents of coffee leaf rust. Biological Control, 63, 62-67. doi: 10.1016/j.biocontrol.2012.06.005
Silva, H. S. A., Oliveira, S. A. S., & Haddad, F. (2011). Uso de imagens digitalizadas em metodologias de seleção para resistência à podridão radicular de mandioca. (Boletim de Pesquisa e Desenvolvimento, 54). Cruz das Almas, BA: EMBRAPA Mandioca e Fruticultura Recuperado de http://www.cnpmf. embrapa.br/publicacoes/boletins/ boletim_54.pdf
Snyder, W. C. & Hansen, H. N. (1953). Species concept, genetics, and pathogenicity in Hypomyces solani. Phytopathology, 44(2), 338-342.
Spadaro, D., & Gullino, M. L. (2005). Improving the efficacy of biocontrol agents against soilborne pathogens. Crop Protection, 24(7), 601-613. doi: 10.1016/j.cropro.2004.11.003
Szandala, E. S., & Backhouse, D. (2001). Suppression of sporulation of Botrytis cinerea by antagonists applied after infection. Australasian Plant Pathology, 30(2), 165-170.
Zhang, X., Zhou, Y., Li, Y., Fu, X., & Wang, Q. (2017). Screening and characterization of endophytic Bacillus for biocontrol of grapevine downy mildew. Crop Protection, 96, 173-179. doi: 10.1016/ j.cropro.2017.02.018
Zheng, Y., Xue, Q.-Y., Xu, L.-L., Xu, Q., Lu, S., Gu, C., & Guo, J.-H. (2011). A screening strategy of fungal biocontrol agents towards Verticillium wilt cotton. Biological Control, 56, 209-2016. doi: 10.1016/j.biocontrol.2010.11.010
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Published
2020-08-07
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Luquine, L. S., Pinheiro, L. R. B., Oliveira, S. A. S. de, & Silva, H. S. A. (2020). Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense. Semina: Ciências Agrárias, 41(5supl1), 2401–2410. https://doi.org/10.5433/1679-0359.2020v41n5supl1p2401
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