Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil
DOI:
https://doi.org/10.5433/1679-0359.2020v41n2p689Keywords:
Babesia vogeli, Rhipicephalus sanguineus, Rainforest biome, Polymerase chain reaction.Abstract
Tick-borne diseases have a significant worldwide impact in the veterinary and medical fields. The Serra da Tiririca State Park (PESET) is an Atlantic forest reserve that is home to a wide variety of wild animal species, but is suffering from unauthorised human activities, such as unregulated construction, such as private dwellings and the introduction of domestic pets. The objective of this study is to determine the prevalence of piroplasms and Hepatozoon spp. in dogs and ticks in the Atlantic Forest reserve. Blood samples were taken from 124 asymptomatic canines and 88 Rhipicephalus sanguineus ticks collected from animals inhabiting the PESET. Molecular techniques targeting fragments of the 18s rRNA gene from piroplasms and Hepatozoon spp. were applied to all samples obtained, and one blood sample (0.81%) positive for Babesia vogeli was discovered. All dogs tested negative to Hepatozoon spp. and no tick sample showed positive results for either PCR assay. Despite the low frequency of B. vogeli infection found in dogs that inhabit the PESET, parasitized dogs could be a source of transmission for new vectors, other dogs and even wild canines that are part of the native fauna of the park.Downloads
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References
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Birkenheuer, A. J., Levy, M. G., Breitschwerdt, E. B. (2003). Development and evaluation of a seminested PCR for detection and differentiation of Babesia gibsoni (Asian genotype) and B. canis DNA in canine blood samples. Journal of Clinical Microbiology, American Society for Microbiology, 41(9), 4172-4177. doi: 10.1128/jcm.41.9.4172-4177.2003
Demoner, L. C., Rubini, A. S., Paduan, K. S., Metzger, B., Antunes, J. M. A. P., Martins, T. F.,… O’Dwyer, L. H. (2013). Investigation of tick vectors of Hepatozoon canis in Brazil. Ticks and Tick-borne Diseases, 4(6), 542-546. doi: 10.1016/j.ttbdis.2013.07.006
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Forlano, M., Scofield, A., Elisei C., Fernandes, K. R., Ewing, S. A., & Massard, C. L. (2005). Diagnosis of Hepatozoon spp. in Amblyomma ovale and its experimental transmission in domestic dogs in Brazil. Veterinary Parasitology, 134(1-2), 1-7. doi: 10.1016/j.vetpar.2005.05.066
Inokuma, H., Okuda, M., Ohno, K., Shimoda, K., & Onishi, T. (2002). Analysis of the 18S rRNA gene sequence of a Hepatozoon detected in two Japanese dogs. Veterinary Parasitology, 106(3), 265-71. doi: 10.1016/s0304-4017(02)00065-1
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Lemos, T. D., Toma, H. K., Assad, R. Q., Silva, A. V. C., Bartolomeu, R. G., & Almosny, N. R. P. (2017). Clinical and hematological evaluation of Rangelia vitalii-naturally infected dogs in southeastern Brazil. Revista Brasileira de Parasitologia Veterinária, 26(3), 307-313. doi: 10.1590/s1984-29612017040
Ramos, R., Ramos, C., Araujo, F., Oliveira, R., Souza, I., Pimentel, D.,... Alves, L. (2010). Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (north-eastern Brazil). Parasitology Research, 107(5), 1115-1120. doi: 10.1007/s00436-010-1979-7
Solano-Gallego, L., Trotta, M., Carl, B., Carcy, B., Caldin, M., & Furlanello, T. (2008). Babesia canis canis and Babesia canis vogeli clinic pathological findings and DNA detection by means of PCR-RFLP in blood from Italian dogs suspected of tick-borne disease. Veterinary Parasitology, 157(3-4), 211-221. doi: 10.1016/j.vetpar.2008.07.024
Sousa, K. C. M., Fernandes, M. P., Herrera, H. M., Freschi, C. R., Machado, R. Z., & André, M. R. (2017). Diversity of piroplasmids among wild and domestic mammals and ectoparasites in Pantanal wetland, Brazil. Ticks Tick Borne Disease, 9(2), 245-253. doi: 10.1016/j.ttbdis.2017.09.010
Temoche, L. C., Assad, R., Seabra-Junior, E. S., Didonet Lemos, T. D., & Almosny, N. R. P. (2018). Frequency of Babesia vogeli in domestic dogs in the metropolitan area of Piura, Peru. Acta Veterinaria Brno, 87(3), 255-260. doi: 10.2754/avb201887030255
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Published
2020-03-06
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Ferreira, M. R., Souza, A. M. de, Campos, S. D. E., Cunha, N. C. da, Silva, B. T. G. da, Silva, N. X. da, & Almosny, N. R. P. (2020). Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil. Semina: Ciências Agrárias, 41(2), 689–696. https://doi.org/10.5433/1679-0359.2020v41n2p689
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