Antimicrobial resistance profile, phage typing and molecular characterization of Salmonella Enteritidis strains from poultry origin
DOI:
https://doi.org/10.5433/1679-0359.2012v33n2p705Keywords:
Salmonella Enteritidis, Phage typing, Antimicrobial resistance, Pulsed field gel electrophoresis (PFGE).Abstract
Salmonella enterica subsp. enterica serovar Enteritidis (SE) is currently responsible for significant losses in the poultry industry with consequences on public health. In the present study, 38 SE isolates from biological material of chicken breeders were characterized using phage typing, antimicrobial resistance profile and pulsed field gel electrophoresis (PFGE). The phage type (PT) 7 and 9 were predominant and 26.3% of the isolates were resistance to nalidixic acid (NAL). The phage typing and analysis of antimicrobial resistance profile showed high discriminatory power (0.85). The PFGE profile of 13 strains with XbaI and SpeI discriminated the SE phage types, as well characterized strains from the same serovar. The results showed the importance to associate different methods for the characterization of SE and suggest that poultry products may have been source of human salmonellosis in the Parana State during the period of study.
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